 | | Vol. 15.34 – 9 September, 2020 |
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| Investigators developed an in vitro disease-in-a-dish model using iPSCs from type 2 diabetes (T2D) patients differentiated into myoblasts (iMyos). They found that T2D iMyos in culture exhibited multiple defects mirroring human disease, including an altered insulin signaling, decreased insulin-stimulated glucose uptake, and reduced mitochondrial oxidation.
[Cell Metabolism] |
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 | | PUBLICATIONSRanked by the impact factor of the journal |
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| Researchers knocked out the main de novo DNA methyltransferase in cardiomyocytes, DNMT3A, in human (h)iPSCs. Functional consequences of DNA methylation-deficiency under control and stress conditions were then assessed in human engineered heart tissue from knockout hiPSC-derived cardiomyocytes.
[Circulation] |
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| Scientists determined whether ESCs were capable of generating lung tissue in Nkx2-1-/- mouse embryos with lung agenesis.
[American Journal of Respiratory and Critical Care Medicine] |
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| Using affinity purification and mass spectrometry analysis, investigators identified Kap1 as an Oct4-binding protein. Silencing of Kap1 reduced the protein levels of Oct4 in ESCs, whereas the overexpression of Kap1 stimulated the levels of Oct4.
[Cell Death & Differentiation] |
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| The authors report that YTHDF2 and YTHDF3, but not YTHDF1, were required for reprogramming of somatic cells into iPSCs.
[Cell Reports] |
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| Scientists applied CRISPR/Cas9 genome editing technology to introduce three RBM20 mutations in iPSCs and differentiate them into iPSC-cardiomyocytes to establish an in vitro model of RBM20 mutant dilated cardiomyopathy.
[Cell Reports] |
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| Researchers determined the therapeutic potential of human (h)iPSCs in a mouse model of VML. Muscles were subjected to excision to generate 30-40% muscle loss. Next, hiPSCs were differentiated toward skeletal myogenic progenitors and used with fibrin hydrogel to reconstruct the lost muscle.
[Molecular Therapy] |
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| The authors provided evidence, for the first time, to prove that iPSCs, being a single somatic cell-derived clone, are recording almost identical mutational profile of the initial cell progenitor.
[Computational and Structural Biotechnology Journal] |
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| Researchers developed an in vitro system of hepatitis B virus (HBV) infection using iPSC-derived liver cells, and showed that epidermal growth factor secreted from liver sinusoidal endothelial cells modulated HBV infection in a dose-dependent manner.
[Scientific Reports] |
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| The authors reveal an important role for astrocytes in Parkinson’s disease pathology and highlight the potential of iPSC-derived cells in disease modeling and drug discovery.
[Scientific Reports] |
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| Scientists used large-scale targeted proteomics to demonstrate that undifferentiated human (h)PSCs express different fatty acid (FA) biosynthesis-related enzymes, including ATP citrate lyase and FA synthase, than those expressed in hPSC-derived cardiomyocytes.
[iScience] |
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| Because of differences in bioreactor expansion efficiency between mouse (m) and human (h) PSCs, researchers investigated if conversion of hPSCs, from the conventional “primed” pluripotent state towards the “naïve” state prevalent in mPSCs, could be used to enhance hPSC production.
[Communications Biology] |
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| The authors provide insights from developmental biology with a comprehensive resource of signaling pathways that in a coordinated manner form embryo-like structures and organoids. The advent of assembloids and multilineage organoids from PSCs opens a new dimension to study paracrine function and multi-tissue interactions in vitro.
[iScience] |
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| Cynata Therapeutics Limited has announced positive efficacy data from a study of its iPSC-derived Cymerus™ mesenchymal stem cells (MSCs) in a preclinical rodent model of idiopathic pulmonary fibrosis.
[Cynata Therapeutics Limited (GlobeNewswire, Inc.)] |
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| | The Francis Crick Institute – London, England, United Kingdom |
| | | University of Washington – Seattle, Washington, United States |
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| | University of Saskatchewan – Saskatoon, Saskatchewan, Canada |
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| | Icahn School of Medicine at Mount Sinai – New York City, New York, United States |
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| | Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai – New York City, New York, United States |
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