ESC & iPSC News Volume 15.42 | Nov 4, 2020

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    2020-11-4 | ESC 15.42


    ESC & iPSC News by STEMCELL Technologies
    Vol. 15.42 – 4 November, 2020
    TOP STORY

    Derivation
    of Airway Basal Stem Cells from Human Pluripotent Stem Cells

    The authors report the directed differentiation of human iPSCs into airway basal cells, a population resembling the stem cell of the airway epithelium.
    [Cell Stem Cell]

    AbstractGraphical Abstract
    Press Release


    Survey Report: Where Is Pluripotent Stem Cell Research Now?
    PUBLICATIONSRanked by the impact factor of the journal

    Humanized
    Neurofibroma Model from Induced Pluripotent Stem Cells Delineates Tumor Pathogenesis and Developmental Origins

    To investigate human neurofibroma pathogenesis, researchers differentiated a series of isogenic patient-specific neurofibromatosis type 1-mutant human iPSCs into Schwannian lineage cells.
    [Journal of Clinical Investigation]

    AbstractGraphical Abstract

    Gene
    Expression and Functional Deficits Underlie TREM2-Knockout Microglia Responses in Human Models of Alzheimer’s Disease

    Scientists profiled microglia differentiated from isogenic, CRISPR-modified TREM2-knockout iPSC lines.
    [Nature Communications]

    Full Article

    Nuclear
    Gene Proximity and Protein Interactions Shape Transcript Covariations in Mammalian Single Cells

    The authors applied a tailored experimental design that eliminated confounding effects of differentiation and cell cycle in studying single-cell RNA sequencing, and report thousands of intrinsically covarying gene pairs in mouse ESCs.
    [Nature Communications]

    Full Article

    Cell-Cycle-Dependent
    ERK Signaling Dynamics Direct Fate Specification in the Mammalian Preimplantation Embryo

    Endogenous tagging of Nanog in ESCs revealed that ERK inhibition promoted enhanced stabilization of NANOG protein after mitosis. Scientists showed that cell cycle, signaling, and differentiation are coordinated during preimplantation development.
    [Developmental Cell]

    Full ArticleGraphical Abstract

    Single-Cell
    RNA-Seq Reveals that CD9 Is a Negative Marker of Glucose-Responsive Pancreatic β-like Cells Derived from Human Pluripotent Stem Cells

    Investigators observed more CD9+C-PEPTIDE+ β cells in the fetal than in the adult cadaveric islets and more Ki67+ proliferating cells among CD9+ fetal β cells. Their experiments showed CD9 as a cell-surface marker for negative enrichment of glucose-responsive β-like cells differentiated from human PSCs.
    [Stem Cell Reports]

    Full ArticleGraphical Abstract

    Leptin
    Receptor Signaling Regulates Protein Synthesis Pathways and Neuronal Differentiation in Pluripotent Stem Cells

    Using a global unbiased proteomics approach, researchers report that embryonic fibroblasts carrying the db/db mutation exhibit metabolic abnormalities, while their reprogrammed iPSCs show altered expression of proteins involved in embryonic development.
    [Stem Cell Reports]

    Full ArticleGraphical Abstract

    Visualization
    and Quantification of Dynamic Intercellular Coupling in Human Embryonic Stem Cells Using Single Cell Sonoporation

    The authors quantify cell–cell permeability based on dye diffusion using mass transport models. Their results revealed heterogeneous intercellular connectivity and a variety of spatiotemporal characteristics of intercellular Ca2+ waves in human ESC colonies induced by sonoporation of single cells.
    [Scientific Reports]

    Full Article

    Functional In Vivo and In Vitro
    Effects of 20q11.21 Genetic Aberrations on hPSC Differentiation

    Investigators examined the functional in vivo and in vitro effects of frequently detected copy number variations at 20q11.21 during early-stage differentiation of human (h)PSCs. Comprehensive transcriptome profiling of abnormal hPSCs revealed that the differential gene expression patterns had a negative effect on differentiation potential.
    [Scientific Reports]

    Full Article

    CRISPR/Cas9
    Mediated Generation and Analysis of N-Terminus Polymorphic Models of Beta2-Adrenoreceptor in Isogenic hPSC Derived Cardiomyocytes

    Scientists used CRISPR/Cas9 gene editing technology in human (h)PSCs to create a unique suite of four isogenic homozygous variants at amino acid positions 16(G/R) and 27(G/Q), which reside in the N-terminus of the β2AR.
    [Molecular Therapy-Methods & Clinical Development]

    AbstractGraphical Abstract

    IFI16
    Promotes Human Embryonic Stem Cell Trilineage Specification through Interaction with p53

    Researchers found the immune regulative protein, gamma-interferon-inducible protein 16 (IFI16) was involved in the regulation of both self-renewal and differentiation gene expression during human ESC trilineage specification through interaction with p53.
    [NPJ Regenerative Medicine]

    Full Article

    The
    PWWP2A Histone Deacetylase Complex Represses Intragenic Spurious Transcription Initiation in mESCs

    Scientists used CAGE-seq to profile all transcription initiation sites in wildtype mouse (m)ESCs and cells lacking PWWP2A/B. Loss of PWWP2A/B enhanced spurious initiation from intragenic sites present in wildtype mESCs, and this effect was associated with increased levels of initiating Pol-II and histone acetylation.
    [iScience]

    AbstractGraphical Abstract


    Andreas Kurtz spearheaded a global stem cell registry to make hPSC data more accessible. Watch the webinar.
    REVIEWS

    Patient
    Derived Stem Cells for Discovery and Validation of Novel Pathogenic Variants in Inherited Retinal Disease

    The authors present an overview of the current state of pathogenic variant discovery in retinal disease and identify some of the remaining barriers. They also explore the utility of new technologies, specifically patient-derived iPSC-based modeling, in further expanding the catalog of disease-causing variants using transcriptome-focused methods.
    [Progress in Retinal and Eye Research]

    Abstract

    INDUSTRY AND POLICY NEWS

    Harnessing
    the Power and Potential of Regenerative Medicine in Canada

    The Stem Cell Network announced $4.3 million in funding to support stem cell and regenerative medicine research projects that will help to deliver improved health for Canadians and will contribute to national economic recovery. Funded projects include stem cell-based research into new therapeutics for type-1 diabetes, cancer, cystic fibrosis, brain and heart conditions.
    [Stem Cell Network]

    Press Release

    FEATURED EVENT

    The American Association for Cancer Research (AACR) Annual Meeting 2021

    April 9 – April 14, 2021
    Washington, DC, United States


    > See All Events

    JOB OPPORTUNITIES

    Project
    Research Scientist – CRISPR/CAS9 Genome Editing

    The Francis Crick Institute – London, England, United Kingdom

    Postdoctoral
    Research Scientist – Stem Cell and Tissue Engineering

    Columbia University Medical Center – New York, New York, United States

    (Senior)
    Stem Cell Biology Scientist

    Ncardia – Leiden, The Netherlands

    Postdoctoral Researcher – Stem Cells / MSCs

    National University of Ireland Galway – Galway, Ireland

    Postdoctoral Position – Brain Endothelium and Astrocytes

    University of California, San Diego- La Jolla, California, United States

    > See All Jobs

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