ESC & iPSC News Volume 16.04 | Feb 3 2021

2021-3-2 | ESC 16.04

ESC & iPSC News by STEMCELL Technologies
Vol. 16.04 – 3 February, 2021

Metabolic Control of DNA Methylation in Naive Pluripotent Cells

The authors showed that LIF-Stat3 signaling induced genomic hypomethylation via metabolic reconfiguration. Stat3−/− ESCs showed decreased α-ketoglutarate production from glutamine, leading to increased Dnmt3a and Dnmt3b expression and DNA methylation.
[Nature Genetics]


Checking your cell quality doesn’t have to be difficult - Genetic screening with the hPSC Genetic Analysis Kit
PUBLICATIONSRanked by the impact factor of the journal

Pathogenic LMNA Variants Disrupt Cardiac Lamina-Chromatin Interactions and De-Repress Alternative Fate Genes

Researchers introduced LMNA mutations from individuals with dilated cardiomyopathy into human (h)iPSCs and found that hiPSC-derived cardiomyocytes, in contrast to hepatocytes or adipocytes, exhibited aberrant nuclear morphology and specific disruptions in peripheral chromatin.
[Cell Stem Cell]

AbstractGraphical Abstract

Single-Cell Transcriptomic Reveals Molecular Diversity and Developmental Heterogeneity of Human Stem Cell-Derived Oligodendrocyte Lineage Cells

Investigators describe a time-course single-cell-transcriptomic analysis of developing human stem cell-derived oligodendrocyte-lineage-cells.
[Nature Communications]

Full Article

3D Bioprinting of High Cell-Density Heterogeneous Tissue Models through Spheroid Fusion within Self-Healing Hydrogels

Scientists bioprinted iPSC-derived cardiac microtissue models with spatially controlled cardiomyocyte and fibroblast cell ratios to replicate the structural and functional features of scarred cardiac tissue that arise following myocardial infarction, including reduced contractility and irregular electrical activity.
[Nature Communications]

Full Article

Cross-Species Single-Cell Transcriptomic Analysis Reveals Pre-Gastrulation Developmental Differences among Pigs, Monkeys, and Humans

The authors constructed a complete single-cell landscape of early embryonic development of pig, considered one of the best host species for human organ generation, and systematically compared its epiblast development with that of human and monkey.
[Cell Discovery]

Full Article

Generation of Human-Induced Pluripotent Stem Cell-Derived Functional Enterocyte-Like Cells for Pharmacokinetic Studies

Scientists demonstrated the generation of enterocyte-like cells by culturing human iPSC-derived intestinal progenitor cells on a collagen vitrigel membrane.
[Stem Cell Reports]

Full Article

Cell-to-Cell Adhesion and Neurogenesis in Human Cortical Development: A Study Comparing 2D Monolayers with 3D Organoid Cultures

The authors compared transcriptome and cellular phenotypes between telencephalic organoids and monolayers generated in parallel from three biologically distinct iPSC lines.
[Stem Cell Reports]

Full Article

Cell Type-Specific Changes in Transcriptomic Profiles of Endothelial Cells, iPSC-Derived Neurons and Astrocytes Cultured on Microfluidic Chips

Investigators analyzed the transcriptomic profiles of co-cultures of human iPSC-derived neurons and rat astrocytes, as well as one-day monocultures of human endothelial cells, cultured on microfluidic chips.
[Scientific Reports]

Full Article

Salicylic Diamines Selectively Eliminate Residual Undifferentiated Cells from Pluripotent Stem Cell-Derived Cardiomyocyte Preparations

Researchers identified salicylic diamines as potent agents exhibiting toxicity to murine and human PSCs but not to cardiomyocytes derived from them.
[Scientific Reports]

Full Article

Explore scientific events this February with the Science Events Calendar.

Defined Matrices Bring IBD to 3D

An immune cell population enriched in inflamed gut tissue is shown to play a role in driving CD44+ intestinal organoid proliferation, while also regulating extracellular matrix deposition and remodeling in a synthetic hydrogel platform.
[Nature Materials]

Full Article

Cell-Based Strategies for IVD Repair: Clinical Progress and Translational Obstacles

Scientists discuss current approaches to disc regeneration, with a particular focus on cell-based therapeutic strategies, including ongoing challenges, and attempts to provide a framework to interpret current data and guide future investigational studies.
[Nature Reviews Rheumatology]


Chromatin Accessibility Dynamics during Cell Fate Reprogramming

Investigators discuss recent findings linking chromatin dynamics to cell fate control. Specifically, chromatin undergoes a binary off/on switch during iPSC reprogramming, closing and opening loci occupied by somatic and pluripotency transcription factors, respectively.
[EMBO Reports]

Full Article

Using Patient-Derived Induced Pluripotent Stem Cells to Identify Risks of Drug-Induced Cardiotoxicity

The authors discuss the current use of patient-specific iPSCs in identifying populations who are at risk to drug-induced cardiotoxicity and their potential applications in future precision medicine practice.
[Arteriosclerosis Thrombosis and Vascular Biology]



Cellino Closes $16M Seed Financing Led by the Engine and Khosla Ventures

Cellino, a personalized regenerative medicine company developing an automated process to generate autologous iPSCs at scale, has announced the closing of a $16M Seed financing round. The round was co-led by The Engine and Khosla Ventures, with participation from Humboldt Fund and 8VC.

Press Release


Cambridge Cancer Epigenetics Club: Tim Somervaille

February 5, 2021

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Faculty Position – Department of Hematology

St. Jude Children’s Research Hospital – Memphis, Tennessee, United States

Postdoctoral Position – Brain Disorder Signaling

University of Southern California – Los Angeles, California, United States

Assistant / Associate Professor – Brain Tumor

University of Utah – Salt Lake City, Utah, United States

Postdoctoral Researcher – Neuroimmunology

University of Luxembourg – Luxembourg City, Luxembourg

PhD Studentship – Bioengineering and Biotechnologies

Queen Mary University of London – London, England, United Kingdom

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