ESC & iPSC News Volume 16.05 | Feb 10 2021







2021-10-2 | ESC 16.05


ESC & iPSC News by STEMCELL Technologies
Vol. 16.05 – 10 February, 2021
TOP STORY

Human Heart-Forming Organoids Recapitulate Early Heart and Foregut Development

Scientists generated complex, highly structured, 3D heart-forming organoids to study genetic defects in vitro.
[Nature Biotechnology]

Abstract

New mTeSR™ Plus hPSC Maintenance Medium
PUBLICATIONSRanked by the impact factor of the journal

H3.3-K27M Drives Neural Stem Cell-Specific Gliomagenesis in a Human iPSC-Derived Model

Researchers engineered human iPSCs to carry an inducible H3.3-K27M allele in the endogenous locus and studied the effects of the mutation in different disease-relevant neural cell types.
[Cancer Cell]

AbstractGraphical Abstract

Biphasic Activation of WNT Signaling Facilitates the Derivation of Midbrain Dopamine Neurons from hESCs for Translational Use

Investigators presented an authentic midbrain dopamine neuron derivation protocol based on a two-step WNT signaling activation strategy that improved expression of midbrain markers, such as Engrailed-1, while minimizing expression of contaminating posterior and anterior lineage markers.
[Cell Stem Cell]

AbstractGraphical Abstract

Sequential CRISPR Gene Editing in Human iPSCs Charts the Clonal Evolution of Myeloid Leukemia and Identifies Early Disease Targets

Scientists combined iPSC and CRISPR-Cas9 technologies to develop a model of the clonal evolution of acute myeloid leukemia.
[Cell Stem Cell]

AbstractPress Release

Fully Defined Human Pluripotent Stem Cell-Derived Microglia and Tri-Culture System Model C3 Production in Alzheimer’s Disease

The authors developed a new approach to derive microglia from human (h)PSCs and built a defined hPSC-derived tri-culture system containing pure populations of hPSC-derived microglia, astrocytes, and neurons to dissect cellular cross-talk along the neuroinflammatory axis in vitro.
[Nature Neuroscience]

Abstract

Defective AMPA-Mediated Synaptic Transmission and Morphology in Human Neurons with Hemizygous SHANK3 Deletion Engrafted in Mouse Prefrontal Cortex

Scientists generated CRISPR/Cas9-engineered human PSCs with complete hemizygous SHANK3 deletion, the most common genetic abnormality in patients, and investigated the synaptic and morphological properties of SHANK3-deficient PSC-derived cortical neurons engrafted in the mouse prefrontal cortex.
[Molecular Psychiatry]

Abstract

Derivation of Peripheral Nociceptive, Mechanoreceptive, and Proprioceptive Sensory Neurons from the Same Culture of Human Pluripotent Stem Cells

The authors describe a chemically defined differentiation protocol that generated all three sensory neuron subtypes from the same starting population, as well as methods to enrich for each individual subtype.
[Stem Cell Reports]

Full ArticleGraphical Abstract

Rictor/mTORC2 Involves Mitochondrial Function in ES Cells Derived Cardiomyocytes via Mitochondrial Connexin 43

Investigators used the model of cardiomyocyte differentiation from mouse ESCs to elucidate the mechanisms for the mitochondrial damage in ESC-derived cardiomyocytes after knockdown of Rictor.
[Acta Pharmacologica Sinica]

Full Article

Cell Therapy with Human Induced Pluripotent Stem Cell-Derived Retinal Pigment Epithelium and Retinal Precursor Cells Prevents Visual Function Loss in a Rat Model of Retinal Degeneration

Researchers determined whether combined transplantation of human iPSC-derived retinal precursor cells (RPC) and retinal pigment epithelial (RPE) cells was superior to RPE or RPC transplantation alone in preserving retinal from degeneration.
[Molecular Therapy-Methods & Clinical Development]

AbstractGraphical Abstract

Sox2 Modulation Increases Naïve Pluripotency Plasticity

Induced pluripotency provides a tool to explore mechanisms underlying establishment, maintenance and differentiation of naïve (n)PSCs. The authors report that self-renewal of nPSCs required minimal Sox2 expression.
[iScience]

AbstractGraphical Abstract

A Human Model of Batten Disease Shows Role of CLN3 in Phagocytosis at the Photoreceptor-RPE Interface

A proportion of CLN3 in human, mouse, and iPSC-retinal pigment epithelium (RPE) cells localized to RPE microvilli, the site of photoreceptor outer segment (POS) phagocytosis. Furthermore, patient-derived CLN3 disease iPSC-RPE cells showed decreased RPE microvilli density and reduced POS binding and ingestion.
[Communications Biology]

Full Article

iPSC-Derived Cardiomyocytes from Patients with Myotonic Dystrophy Type 1 Have Abnormal Ion Channel Functions and Slower Conduction Velocities

Researchers developed a disease model using iPSC-derived cardiomyocytes from a healthy individual and two Myotonic Dystrophy type 1 patients with different CTG repeats lengths and clinical history.
[Scientific Reports]

Full Article

Are Your Pluripotent Stem Cells What You Think They Are? Explore Now.
REVIEWS

Ubiquitous Chromatin Modifiers in Congenital Retinal Diseases: Implications for Disease Modeling and Regenerative Medicine

Combining knowledge of epigenetics and PSCs is a promising avenue because epigenetic factors cooperate with eye field transcription factors to direct PSC fate – a foundation for congenital retinal disease modeling and cell therapy.
[Trends in Molecular Medicine]

Abstract

INDUSTRY AND POLICY NEWS

Leading Innovators Collaborate to Provide New, Patient Representative Cell Culture Solutions for Smarter In Vitro Research

One of the biggest challenges in drug development and personalized medicine is how to model the cellular microenvironment accurately. Cellaria and BioLamina are pursuing this challenge by collaborating to develop more defined reprogramming methods, large-scale iPSC expansions, and lineage differentiation for human cells and animal cells for vertebrate and non-vertebrate species.
[Cellaria Inc. and BioLamina (Businesswire, Inc.)]

Press Release

FEATURED EVENT

Virtual PSC Maintenance and Cell Quality Training Course – April

April 12 – 14, 2021
Virtual

> See All Events

JOB OPPORTUNITIES

Postdoctoral Positions – Hematopoiesis, Leukemia, Cancer, Human Pluripotent Stem Cells

Icahn School of Medicine at Mount Sinai – New York, New York, United States

Postdoctoral Researcher – 3D In Vitro Systems for Respiratory Bacterial Infections

NCCR AntiResist – Basel, Switzerland

Postdoctoral Position – Brain Disorder Signaling

University of Southern California – Los Angeles, California, United States

Faculty Position – Department of Hematology

St. Jude Children’s Research Hospital – Memphis, Tennessee, United States

Postdoctoral Researcher – Neuroimmunology

University of Luxembourg – Luxembourg City, Luxembourg

> See All Jobs

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