ESC & iPSC News Volume 16.05 | Feb 10 2021

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    2021-10-2 | ESC 16.05


    ESC & iPSC News by STEMCELL Technologies
    Vol. 16.05 – 10 February, 2021
    TOP STORY

    Human Heart-Forming Organoids Recapitulate Early Heart and Foregut Development

    Scientists generated complex, highly structured, 3D heart-forming organoids to study genetic defects in vitro.
    [Nature Biotechnology]

    Abstract

    New mTeSRâ„¢ Plus hPSC Maintenance Medium
    PUBLICATIONSRanked by the impact factor of the journal

    H3.3-K27M Drives Neural Stem Cell-Specific Gliomagenesis in a Human iPSC-Derived Model

    Researchers engineered human iPSCs to carry an inducible H3.3-K27M allele in the endogenous locus and studied the effects of the mutation in different disease-relevant neural cell types.
    [Cancer Cell]

    AbstractGraphical Abstract

    Biphasic Activation of WNT Signaling Facilitates the Derivation of Midbrain Dopamine Neurons from hESCs for Translational Use

    Investigators presented an authentic midbrain dopamine neuron derivation protocol based on a two-step WNT signaling activation strategy that improved expression of midbrain markers, such as Engrailed-1, while minimizing expression of contaminating posterior and anterior lineage markers.
    [Cell Stem Cell]

    AbstractGraphical Abstract

    Sequential CRISPR Gene Editing in Human iPSCs Charts the Clonal Evolution of Myeloid Leukemia and Identifies Early Disease Targets

    Scientists combined iPSC and CRISPR-Cas9 technologies to develop a model of the clonal evolution of acute myeloid leukemia.
    [Cell Stem Cell]

    AbstractPress Release

    Fully Defined Human Pluripotent Stem Cell-Derived Microglia and Tri-Culture System Model C3 Production in Alzheimer’s Disease

    The authors developed a new approach to derive microglia from human (h)PSCs and built a defined hPSC-derived tri-culture system containing pure populations of hPSC-derived microglia, astrocytes, and neurons to dissect cellular cross-talk along the neuroinflammatory axis in vitro.
    [Nature Neuroscience]

    Abstract

    Defective AMPA-Mediated Synaptic Transmission and Morphology in Human Neurons with Hemizygous SHANK3 Deletion Engrafted in Mouse Prefrontal Cortex

    Scientists generated CRISPR/Cas9-engineered human PSCs with complete hemizygous SHANK3 deletion, the most common genetic abnormality in patients, and investigated the synaptic and morphological properties of SHANK3-deficient PSC-derived cortical neurons engrafted in the mouse prefrontal cortex.
    [Molecular Psychiatry]

    Abstract

    Derivation of Peripheral Nociceptive, Mechanoreceptive, and Proprioceptive Sensory Neurons from the Same Culture of Human Pluripotent Stem Cells

    The authors describe a chemically defined differentiation protocol that generated all three sensory neuron subtypes from the same starting population, as well as methods to enrich for each individual subtype.
    [Stem Cell Reports]

    Full ArticleGraphical Abstract

    Rictor/mTORC2 Involves Mitochondrial Function in ES Cells Derived Cardiomyocytes via Mitochondrial Connexin 43

    Investigators used the model of cardiomyocyte differentiation from mouse ESCs to elucidate the mechanisms for the mitochondrial damage in ESC-derived cardiomyocytes after knockdown of Rictor.
    [Acta Pharmacologica Sinica]

    Full Article

    Cell Therapy with Human Induced Pluripotent Stem Cell-Derived Retinal Pigment Epithelium and Retinal Precursor Cells Prevents Visual Function Loss in a Rat Model of Retinal Degeneration

    Researchers determined whether combined transplantation of human iPSC-derived retinal precursor cells (RPC) and retinal pigment epithelial (RPE) cells was superior to RPE or RPC transplantation alone in preserving retinal from degeneration.
    [Molecular Therapy-Methods & Clinical Development]

    AbstractGraphical Abstract

    Sox2 Modulation Increases Naïve Pluripotency Plasticity

    Induced pluripotency provides a tool to explore mechanisms underlying establishment, maintenance and differentiation of naïve (n)PSCs. The authors report that self-renewal of nPSCs required minimal Sox2 expression.
    [iScience]

    AbstractGraphical Abstract

    A Human Model of Batten Disease Shows Role of CLN3 in Phagocytosis at the Photoreceptor-RPE Interface

    A proportion of CLN3 in human, mouse, and iPSC-retinal pigment epithelium (RPE) cells localized to RPE microvilli, the site of photoreceptor outer segment (POS) phagocytosis. Furthermore, patient-derived CLN3 disease iPSC-RPE cells showed decreased RPE microvilli density and reduced POS binding and ingestion.
    [Communications Biology]

    Full Article

    iPSC-Derived Cardiomyocytes from Patients with Myotonic Dystrophy Type 1 Have Abnormal Ion Channel Functions and Slower Conduction Velocities

    Researchers developed a disease model using iPSC-derived cardiomyocytes from a healthy individual and two Myotonic Dystrophy type 1 patients with different CTG repeats lengths and clinical history.
    [Scientific Reports]

    Full Article

    Are Your Pluripotent Stem Cells What You Think They Are? Explore Now.
    REVIEWS

    Ubiquitous Chromatin Modifiers in Congenital Retinal Diseases: Implications for Disease Modeling and Regenerative Medicine

    Combining knowledge of epigenetics and PSCs is a promising avenue because epigenetic factors cooperate with eye field transcription factors to direct PSC fate – a foundation for congenital retinal disease modeling and cell therapy.
    [Trends in Molecular Medicine]

    Abstract

    INDUSTRY AND POLICY NEWS

    Leading Innovators Collaborate to Provide New, Patient Representative Cell Culture Solutions for Smarter In Vitro Research

    One of the biggest challenges in drug development and personalized medicine is how to model the cellular microenvironment accurately. Cellaria and BioLamina are pursuing this challenge by collaborating to develop more defined reprogramming methods, large-scale iPSC expansions, and lineage differentiation for human cells and animal cells for vertebrate and non-vertebrate species.
    [Cellaria Inc. and BioLamina (Businesswire, Inc.)]

    Press Release

    FEATURED EVENT

    Virtual PSC Maintenance and Cell Quality Training Course – April

    April 12 – 14, 2021
    Virtual

    > See All Events

    JOB OPPORTUNITIES

    Postdoctoral Positions – Hematopoiesis, Leukemia, Cancer, Human Pluripotent Stem Cells

    Icahn School of Medicine at Mount Sinai – New York, New York, United States

    Postdoctoral Researcher – 3D In Vitro Systems for Respiratory Bacterial Infections

    NCCR AntiResist – Basel, Switzerland

    Postdoctoral Position – Brain Disorder Signaling

    University of Southern California – Los Angeles, California, United States

    Faculty Position – Department of Hematology

    St. Jude Children’s Research Hospital – Memphis, Tennessee, United States

    Postdoctoral Researcher – Neuroimmunology

    University of Luxembourg – Luxembourg City, Luxembourg

    > See All Jobs

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