ESC & iPSC News Volume 16.07 | Feb 24 2021

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    2020-02-24 | ESC 16.07


    ESC & iPSC News by STEMCELL Technologies
    Vol. 16.07 – 24 February, 2021
    TOP STORY

    Dissecting the Impact of Regional Identity and the Oncogenic Role of Human-Specific NOTCH2NL in an hESC Model of H3.3G34R-Mutant Glioma

    The authors developed a human ESC-based model of H3.3G34R-mutant glioma that recapitulated the key features of the tumors with cell-type specificity to forebrain interneuronal progenitors but not hindbrain precursors.
    [Cell Stem Cell]

    AbstractGraphical Abstract

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    PUBLICATIONSRanked by the impact factor of the journal

    Formative Pluripotent Stem Cells Show Features of Epiblast Cells Poised for Gastrulation

    Scientists report the establishment of self-renewing formative PSCs which manifest features of epiblast cells poised for gastrulation.
    [Cell Research]

    Full Article

    Rbbp4 Suppresses Premature Differentiation of Embryonic Stem Cells

    The authors showed that deficiency of retinoblastoma binding protein 4 (RBBP4), a component of the Polycomb repressive complex 2 in ESCs led to spontaneous differentiation into mesendodermal lineages.
    [Stem Cell Reports]

    Full ArticleGraphical Abstract

    Common Genetic Variation in Humans Impacts In Vitro Susceptibility to SARS-CoV-2 Infection

    Researchers applied human iPSC-based models and CRISPR-engineering to explore the host genetics of SARS-CoV-2.
    [Stem Cell Reports]

    AbstractFull ArticleGraphical Abstract

    A Synthetic mRNA Cell Reprogramming Method Using CYCLIN D1 Promotes DNA Repair Generating Improved Genetically Stable Human Induced Pluripotent Stem Cells

    Investigators demonstrated that CYCLIN D1 made iPSCs have lower multitelomeric signal, reduced double‐strand DNA breaks, correct nuclear localization of RAD51 protein expression, and reduced SNP changes per chromosome, compared with the classical reprogramming method using C‐MYC.
    [Stem Cells]

    Abstract

    Large‐Scale Generation of Megakaryocytes from Human Embryonic Stem Cells Using Transgene‐Free and Stepwise Defined Suspension Culture Conditions

    Scientists defined an optimal, stepwise and completely xeno‐free culture protocol for the generation of megakaryocytes from human ESCs.
    [Cell Proliferation]

    Full Article

    Bioabsorbable Nerve Conduits Three-Dimensionally Coated with Human Induced Pluripotent Stem Cell-Derived Neural Stem/Progenitor Cells Promote Peripheral Nerve Regeneration in Rats

    Researchers investigated the therapeutic potential of nerve conduits coated with human iPSC-derived neurospheres in rat sciatic nerve defects.

    Full Article

    Genome-Wide CRISPR/Cas9-Knockout in Human Induced Pluripotent Stem Cell (iPSC)-Derived Macrophages

    Scientists present an efficient, lentiviral transduction protocol for delivery of CRISPR/Cas9 to macrophages derived from human iPSCs with efficiencies close to 100%.
    [Scientific Reports]

    Full Article

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    REVIEWS

    Engineering Organoids

    The authors investigate cell surface and genetic engineering approaches, and discuss stem cell niche engineering based on the design of matrices that allow spatiotemporal control of organoid growth and shape-guided morphogenesis.
    [Nature Reviews Materials]

    Full Article

    Direct Cell Reprogramming: Approaches, Mechanisms and Progress

    Scientists discuss the evolution of direct reprogramming from a transcription factor-based method to a small-molecule-driven approach, the recent progress in enhancing reprogrammed cell maturation, and the challenges associated with in vivo direct reprogramming for translational applications.
    [Nature Reviews Molecular Cell Biology]

    Abstract

    Development and Application of Endothelial Cells Derived from Pluripotent Stem Cells in Microphysiological Systems Models

    Investigators highlight how knowledge of vascular endothelial cell (EC) development in vivo is used to differentiate iPSCs into ECs.
    [Frontiers in Cardiovascular Medicine]

    Full Article

    INDUSTRY AND POLICY NEWS

    Century Therapeutics Significantly Expands Capabilities with New Operational, Laboratory and Manufacturing Facilities in Pennsylvania and New Jersey

    Century Therapeutics announced a significant expansion of its operational and laboratory space in Philadelphia, as well as progress on its manufacturing facility in Branchburg, NJ, paving the way for a strong technical foundation as the company scales up its in-house research and development capabilities.
    [Century Therapeutics (Cision US Inc.)]

    Press Release

    FEATURED EVENT

    Wisconsin Stem Cell Symposium: The Single Cell Revolution in Stem Cells

    April 14, 2021
    Virtual

    > See All Events

    JOB OPPORTUNITIES

    Postdoctoral Fellow – Genomics of Endocrine Traits

    Children’s Hospital of Philadelphia – Philadelphia, Pennsylvania, United States

    Postdoctoral Position – Hematopoietic Development

    Icahn School of Medicine at Mount Sinai – New York, New York, United States

    Postdoctoral Researcher – 3D In Vitro Systems for Respiratory Bacterial Infections

    NCCR AntiResist – Basel, Switzerland

    Postdoctoral Positions – Hematopoiesis, Leukemia, Cancer, Human Pluripotent Stem Cells

    Icahn School of Medicine at Mount Sinai – New York, New York, United States

    Postdoctoral Position – Brain Disorder Signaling

    University of Southern California – Los Angeles, California, United States

    > See All Jobs

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