 | | Vol. 16.30 – 4 August, 2021 |
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| Investigators developed a non-invasive label-free live cell imaging platform to predict the efficiency of human PSC (hPSC) differentiation into cardiomyocytes. Autofluorescence imaging of metabolic co-enzymes was performed under varying differentiation conditions across five hPSC lines.
[Nature Communications] |
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 | | PUBLICATIONSRanked by the impact factor of the journal |
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| Researchers showed that LIN28 displayed dynamic temporal and spatial expression during murine embryo development. In cultured PSCs, loss of LIN28 led to nucleolar stress and activation of a 2-cell/4-cell-like transcriptional program characterized by the expression of endogenous retrovirus genes.
[Protein & Cell] |
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| The authors showed in mouse embryonic ovaries that germ cell–intrinsic β-catenin activity maintained pluripotency and that its repression was essential to allow differentiation and meiosis entry in a timely manner.
[Proceedings of the National Academy of Sciences of the United States of America] |
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| Scientists performed mitochondrial DNA deep sequencing during the transition from parental fibroblasts to reprogrammed iPSCs and to differentiated neuronal precursor cells obtained from controls and patients affected by mitochondrial disorders.
[Stem Cell Reports] |
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| Researchers used mouse ESCs, which grew in 3D colonies, as a model to investigate division in 3D and observed that, at the periphery of 3D colonies, ESCs displayed high spindle mobility and divided asymmetrically.
[Journal of Cell Science] |
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| Investigators generated a homozygous KCNQ1 knockout human ESC line using episomal vector-based CRISPR/Cas9 system. This generated cell line presented typical stem cells colony morphology, maintained high pluripotency and normal karyotype, also was able to differentiate into all three germ layers in vivo.
[Stem Cell Research] |
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| The authors inserted CRISPRi cassette into the AAVS1 locus of hiPSC lines, which resulted in homozygous knock-in with an otherwise unaltered genome. Expression of dCas9KRAB protein, pluripotency and the ability to differentiate into all three embryonic germ layers were validated.
[Stem Cell Research] |
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| Scientists showed that endogenous stimuli and VEGF were sufficient to induce robust human PSC-derived hematopoiesis, intensive generation of hematopoietic progenitors, maturation of blood cells and the emergence of definitive precursor cells including those that were phenotypically identical to early human embryonic hematopoietic stem cells.
[Journal of Cellular and Molecular Medicine] |
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| An ovarian cancer cell line, PEO4, was reprogrammed into iPSCs using the classical four factors OCT4, SOX2, KLF4 and MYC using lentivirus transduction.
[International Journal of Biochemistry & Cell Biology] |
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| The authors highlight the recent advances and remaining challenges to generating an neuromuscular junction capable of eliciting contraction of stem cell-derived skeletal muscle in vitro.
[Muscle & Nerve] |
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| Fate Therapeutics, Inc. announced that the first patient has been treated with FT819, an off-the-shelf chimeric antigen receptor (CAR) T-cell therapy targeting CD19+ malignancies. FT819 is the first-ever CAR T-cell therapy derived from a clonal master iPSC line.
[Fate Therapeutics, Inc.] |
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| September 13 – 15, 2021
Virtual |
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| | MSH Medical School Hamburg – Hamburg, Germany |
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| | King’s College London – London, England, United States |
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| | Dalhousie University – Halifax, Nova Scotia, Canada |
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| | UCLA Jules Stein Eye Institute – Los Angeles, California, United States |
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| | University of Cambridge – Cambridge, England, United Kingdom |
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