 | | Vol. 16.33 – 25 August, 2021 |
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| Researchers used screens to identify a compound, 5′-iodo-2′-deoxyuridine, that increased gene expression noise in mouse ESCs in culture without changing the overall rate of transcription of most genes.
[Science] |
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 | | PUBLICATIONSRanked by the impact factor of the journal |
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| Scientists demonstrated highly abnormal and epileptiform-like activity in organoids derived from iPSCs from individuals with Rett syndrome.
[Nature Neuroscience] |
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| Investigators developed an approach to form organoid from human iPSCs which, when combined with real-time label-free Raman spectroscopy of biochemical composition changes and confocal light scattering spectroscopic microscopy of chromatin transition, allowed for monitoring live differentiating organoids without the need to sacrifice a sample.
[Science Advances] |
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| Researchers showed that differentiating human iPSC-derived lateral plate mesoderm with BMP4, RA and VEGF could generate a premature form of epicardial cells expressing WT1, TBX18, SEMA3D, and SCX within seven days.
[Nature Communications] |
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| The authors demonstrated that iPSC-tenocyte grafting contributed to motor function recovery after Achilles tendon injury in rats via engraftment and paracrine effects.
[Nature Communications] |
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| Scientists used a novel chromatin RNA in situ reverse transcription sequencing approach to profile long noncoding RNAs in the Oct4 promoter.
[Genome Biology] |
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| Investigators showed that large deletions were ubiquitous but were dependent on editing sites and cell types. Human primary T cells displayed more significant deletions than hematopoietic stem and progenitor cells, whereas they observed low levels in iPSCs.
[Genome Biology] |
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| To determine whether SAGA and ATAC had redundant or specific functions, the authors compared the effects of histone acetyltransferase inactivation in each complex with that of inactivation of either SAGA or ATAC core subunits in mouse ESCs.
[Cell Reports] |
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| Researchers demonstrated generation of patient-derived OPA1 c.1334G>A: p.R445H mutant iPSCs, followed by correction of OPA1 through CRISPR/Cas9 guided homology directed repair and evaluated the effect of OPA1 correction on mitochondrial homeostasis.
[Molecular Therapy-Nucleic Acids] |
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| Scientists reported that the de novo DNA methyltransferases DNMT3A/3B were necessary for XIST repression in female human PSCs. They found that the deletion of both genes, but not the individual genes, inhibited XIST silencing, maintained the heterochromatin mark of H3K27me3, and did not cause global overdosage in X-linked genes.
[Stem Cell Reports] |
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| For the purpose of easy monitoring of differentiation efficiencies into pancreatic progenitors and insulin-producing cells, investigators generated new reporter lines by knocking in a P2A-H-2Kk-F2A-GFP2 reporter gene into the SOX9-locus and a P2A-mCherry reporter gene into the INS-locus mediated by CRISPR/CAS9-technology.
[Stem Cell Reviews and Reports] |
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| Researchers isolated and characterized patient-derived ES primary cell cultures and daughter cancer stem-like cells to identify biomarkers of high-risk disease and candidate therapeutic targets for patients with Ewing sarcoma.
[Cellular Oncology] |
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| The authors demonstrated co-localization of CTCF with Polycomb-repressed chromatin marked by H3K27me3 in pluripotent cells, progressively decreasing in neural precursor cells and differentiated neurons.
[Scientific Reports] |
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| Scientists review the recent advances and possible future directions in the development of in vitro models to better understand human embryogenesis from peri-implantation to gastrulation.
[Trends in Cell Biology] |
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| Researchers from the University of Pittsburgh Liver Research Center, McGowan Institute for Regenerative Medicine, and Carnegie Mellon University’s Computational Biology Department have been awarded almost $1.5 million from the National Science Foundation to develop new approaches for producing liver organoids — tiny, lab-grown human organs that have potential for disease modeling, drug discovery, and transplantation.
[University of Pittsburg] |
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| November 15 – 19, 2021
Virtual |
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| | Stanford University – La Jolla, California, United States |
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| | Michigan State University – East Lansing, Michigan, United States |
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| | Danaher – Toronto, Ontario, Canada |
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| | The Scripps Research Institute – La Jolla, California, United States |
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| | MSH Medical School Hamburg – Hamburg, Germany |
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