ESC & iPSC News Volume 16.37 | Sep 22 2021

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    2021-09-22 | ESC 16.37


    ESC & iPSC News by STEMCELL Technologies
    Vol. 16.37 – 22 September, 2021
    TOP STORY

    Parallel Characterization of Cis-Regulatory Elements for Multiple Genes Using CRISPRpath

    The authors described CRISPRpath, a scalable screening strategy for parallelly characterizing cis-regulatory elements of genes linked to the same biological pathway and converging phenotypes.
    [Science Advances]

    Full Article

    Watch Video Recordings from the Nature Research Round Table on hPSC Quality
    PUBLICATIONSRanked by the impact factor of the journal

    IQSEC2 Mutation Associated with Epilepsy, Intellectual Disability, and Autism Results in Hyperexcitability of Patient-Derived Neurons and Deficient Synaptic Transmission

    Investigators used iPSC technology to obtain hippocampal neurons to investigate the neuropathology of IQSEC2-mediated disease. The neurons were characterized at three-time points during differentiation to assess developmental progression.
    [Molecular Psychiatry]

    Full Article

    Reconstructing Aspects of Human Embryogenesis with Pluripotent Stem Cells

    Researchers attempted to model human early embryo development with expanded PSCs (EPSCs) in 3-dimensions. They defined a protocol that allowed them to generate self-organizing cystic structures from human EPSCs that displayed some hallmarks of human early embryogenesis.
    [Nature Communications]

    Full Article

    A High-Risk Retinoblastoma Subtype with Stemness Features, Dedifferentiated Cone States and Neuronal/Ganglion Cell Gene Expression

    Using multi-omics data, scientists demonstrated the existence of two retinoblastoma subtypes. They expressed markers of less differentiated cone together with neuronal/ganglion cell markers with marked inter- and intra-tumor heterogeneity.
    [Nature Communications]

    Full Article

    CRISPR/Cas9 Mediated Gene Correction Ameliorates Abnormal Phenotypes in Spinocerebellar Ataxia Type 3 Patient-Derived Induced Pluripotent Stem Cells

    Spinocerebellar ataxia type 3 (SCA3)-iPSCs could be corrected by the replacement of the abnormal CAG expansions with normal repeats using CRISPR/Cas9-mediated homologous recombination strategy. Corrected SCA3-iPSCs retained pluripotent and normal karyotype, which could be differentiated into a neural stem cell and neuronal cells.
    [Translational Psychiatry]

    Full Article

    A Microfluidic Approach to Rescue ALS Motor Neuron Degeneration Using Rapamycin

    The authors presented a transgenic, TAR DNA-binding protein-43 (TDP-43)-A315T, mouse model expressing an Amyotrophic Lateral Sclerosis (ALS) phenotype and demonstrated the presence of ubiquitinated cytoplasmic TDP-43 aggregates with > 80% cell death by 28 days post differentiation in vitro.
    [Scientific Reports]

    Full Article

    Effects of 2,2′,4,4′-Tetrabromodiphenyl Ether on the Development of Mouse Embryonic Stem Cells

    To explore the developmental toxicity of 2,2′,4,4′-Tetrabromodiphenyl ether (BDE47), mouse ESCs, which are ideal models for testing the developmental toxicity of environmental contaminants in vitro, were exposed to BDE47 for 24 hours or 48 hours in this study.
    [Reproductive Toxicology]

    Abstract

    Cell Differentiation in the Cardiac Embryonic Stem Cell Test (ESTc) Is Influenced by the Oxygen Tension in Its Underlying Embryonic Stem Cell Culture

    Researchers investigated the chemical sensitivity of the ESTc carried out under 20% O2, using ESCs cultured under either 20% O2 or 5% O2. ESC viability was more sensitive to valproic acid but less sensitive to flusilazole when cultured under 5% versus 20% O2.
    [Toxicology in Vitro]

    Abstract

    The Anti-Tumor Effect of Induced Pluripotent Stem Cells against Submandibular Gland Carcinoma in Rats Is Achieved via Modulation of the Apoptotic Response and the Expression of Sirt-1, TGF-β, and MALAT-1 in Cancer Cells

    The authors investigated the contribution of the Bax, Sirt-1, TGF-β, and MALAT genes and/or their protein expression to the pathogenesis of submandibular carcinogenesis before and after iPSCs treatment.
    [Molecular and Cellular Biochemistry]

    AbstractGraphical Abstract

    Generation of ‘Designer Erythroblasts’ Lacking One or More Blood Group Systems from CRISPR/Cas9 Gene-Edited Human-Induced Pluripotent Stem Cells

    Researchers developed erythroblasts with custom phenotypes using CRISPR/Cas9 gene-editing of a human iPSC parent line for the blood group system genes: RHAG, GYPB and XK.
    [STAR Protocols]

    Full Article

    Using piggyBac Transposon Gene Expression Vectors to Transfect Zscan5b Gene into Mouse Pluripotent Stem Cells

    Investigators provided a detailed protocol for the transfection of mouse ESCs and mouse-iPSCs with a Zscan5b constitutive expression plasmid, using the piggyBac transposon gene expression vector system.
    [STAR Protocols]

    AbstractFull ArticleGraphical Abstract

    Organoids: Nature Research Round Table
    REVIEWS

    iPSC Toolbox for Understanding and Repairing Disrupted Brain Circuits in Autism

    The authors discuss how deficits in neurodevelopmental processes may lead to abnormal brain connectivity and how iPSC-based models can be used to identify abnormal connections and to gain insights into underlying cellular and molecular mechanisms to develop novel therapeutics.
    [Molecular Psychiatry]

    Abstract

    Emerging Roles of Bromodomain Protein 4 in Regulation of Stem Cell Identity

    Scientists review the role of bromodomain protein 4 in complex gene regulatory networks during three specific states of stem cell transitions: cell differentiation, cell reprogramming, and transdifferentiation.
    [Stem Cells]

    AbstractFull Article

    INDUSTRY AND POLICY NEWS

    International Regenerative Medicine Expert Will Lead CSU Translational Medicine Institute

    Dr. Frank Barry has been selected as the new director of the C. Wayne McIlwraith Translational Medicine Institute at Colorado State University. Barry’s research and interests include stem cell biology and the development of cell-based repair strategies for osteoarthritis.
    [Colorado State University]

    Press Release

    Researchers Awarded $31 Million for Clinical Trials to Treat Stroke, Heart Failure, Brain Cancer

    The California Institute for Regenerative Medicine has awarded $31 million to three Stanford researchers to launch trials of treatments for common diseases. Four other Stanford researchers also received a total of $4.55 million.
    [Stanford Medicine]

    Press Release

    FEATURED EVENT

    ESGCT European Society for Gene and Cell Therapies

    October 19 – 22, 2021
    Virtual

    > See All Events

    JOB OPPORTUNITIES

    Postdoctoral Neuronal Cell Biologist – Functional CRISPRi Screening of Parkinson’s Gene in Human iPSC Neurons

    Wellcome Sanger Institute – Cambridge, England, United Kingdom

    Postdoctoral Position – Stem Cells and Disease Modeling

    King Abdullah University of Science and Technology – Thuwal, Saudi Arabia

    Senior Scientist – Neural Stem Cell Biology

    MaxWell Biosystems – Zurich, Switzerland

    Head of Laboratory – Cellular Therapies

    NHS Blood and Transplant – Oxford, England, United Kingdom

    Research Associate – Organogenesis, Stem Cells, and Regenerative Medicine

    University of Southern California – Los Angeles, California, United States

    > See All Jobs

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