 | | Vol. 16.38 – 29 September, 2021 |
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| Researchers identified a novel lncRNA Discn as the guardian of RPA availability in stem cells. Discn loss caused massive genome instability in mouse ESCs and neural stem/progenigor cells.
[Nature Communications] |
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 | | PUBLICATIONSRanked by the impact factor of the journal |
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| The authors followed changes in gene expression, chromatin accessibility, and nuclear structure associated with mouse ESC differentiation and X inactivation by integrating allele-specific data from these three modalities obtained by high-throughput single-cell RNA-seq, ATAC-seq, and Hi-C.
[Genome Biology] |
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| Scientists generated retinal organoids from achromatopsia patient iPSCs carrying ATF6 disease variants and from gene-edited ATF6 null human ESCs, and found that achromatopsia patient and ATF6 null retinal organoids failed to form cone structures concomitant with loss of cone phototransduction gene expression, while rod photoreceptors developed normally.
[Proceedings of the National Academy of Sciences of the United States of America] |
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| Researchers showed that Bouncer’s homolog in mammals, Sperm Acrosome Associated 4 (SPACA4), was required for efficient fertilization in mice. Male knockout mice were severely subfertile, and sperm lacking SPACA4 failed to fertilize wild-type eggs in vitro.
[Proceedings of the National Academy of Sciences of the United States of America] |
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| Airway epithelial patches were engineered by differentiating human iPSC-derived airway progenitors into physiological proportions of ciliated and goblet cells on a silk fibroin–collagen vitrigel membrane composite biomaterial for transplantation in porcine tracheal defects ex vivo and in vivo.
[Advanced Healthcare Materials] |
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| Sequencing of mitochondrial DNA (mtDNA) of 26 iPSC clones did not reveal evidence for de novo mutagenesis, or for any selection processes during reprogramming or differentiation. Culture expansion, however, selected against putatively actionable mtDNA mutations.
[Stem Cell Reports] |
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| Investigators demonstrated that microsatellite alterations were elevated in both mouse and human iPSCs, and identified a type of human iPSC in which these alterations were considerably reduced.
[Stem Cell Reports] |
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| The in vitro and in vivo cartilage and bone tissue formation potencies of human PSCs were investigated. The iPSCs were specified towards the mesodermal lineage and differentiated towards chondrocytes, which subsequently self-assembled into cartilaginous organoids.
[Stem Cell Research & Therapy] |
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| The authors developed reproducible methods to derive, from human PSCs, a population containing monocyte progenitors able to generate functional osteoclast cells. Within this population, they identified cells with monocyte and osteoclast progenitor activity based on CD11b and CD14 expression.
[Blood Advances] |
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| Scientists generated iPSCs from a normal individual by electroporation of peripheral blood mononuclear cells, and further generated a SCN1B-knockout human iPSC line via CRISPR/Cas9 gene editing.
[Stem Cell Research] |
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| Potential interactors of epithelial cell adhesion molecule (EpCAM) were identified in murine F9 teratoma cells using a SILAC-based proteomic approach. Endocytosis and membrane recycling were means of regulation of EpCAM protein levels during differentiation of ESCs and epithelial-to-mesenchymal transition induction in carcinoma cells.
[iScience] |
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| The authors used human iPSCs and generated a developmental timeline by differentiating sporadic amyotrophic lateral sclerosis (sALS) iPSCs to neural progenitors and to motor neurons and comparing mitochondrial parameters with familial ALS and control cells at each developmental stage.
[Scientific Reports] |
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| Researchers developed a robust method to generate 3D, scaffold-free, hyaline cartilage tissue constructs from human ESCs that were composed of numerous chondrocytes in lacunae, embedded in an extracellular matrix containing Type II collagen, sulphated glycosaminoglycans and Aggrecan.
[Scientific Reports] |
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| The authors summarize the increasingly refined and diverse CRISPR toolkit for the latter forms of gene perturbation in human PSCs and their downstream applications.
[CRISPR Journal] |
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| Scientists describe basic knowledge of T cell biology, applications in adoptive cell therapy, the use of iPSCs as a new source of T cells and current differentiation strategies used to generate T cells as well as recent advances in genome engineering to produce next-generation off-the-shelf T cells with improved effector functions.
[Frontiers in Immunology] |
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| Bone Therapeutics and Implant Therapeutics announced the signing of a research evaluation agreement. The agreement will enable Bone Therapeutics to access, evaluate and materially transfer Implant Therapeutics’ iPSC lines, media, differentiation protocols and expertise.
[Bone Therapeutics] |
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| November 1 – 3, 2021
Virtual |
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| | University of Copenhagen – Copenhagen, Denmark |
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| | Imperial College London – London, England, United Kingdom |
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| | University of Helsinki – Helsinki, Finland |
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| | Wellcome Sanger Institute – Cambridge, England, United Kingdom |
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| | King Abdullah University of Science and Technology – Thuwal, Saudi Arabia |
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