ESC & iPSC News Volume 16.43 | Nov 3 2021

May 12, 2022

127

2021-11-03 | ESC 16.43

ESC & iPSC News by STEMCELL Technologies

Vol. 16.43 – 3 November, 2021

TOP STORY

Ribonuclease Recruitment Using a Small Molecule Reduced c9ALS/FTD r(G₄C₂) Repeat Expansion In Vitro and In Vivo ALS Models

Scientists demonstrated that targeted small-molecule degradation of an expanded G₄C₂ RNA repeat was a strategy for mitigating amyotrophic lateral sclerosis and frontotemporal dementia (c9ALS/FTD)-associated pathologies and studying disease-associated pathways in preclinical models.
[Science Translational Medicine]

Full Article

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PUBLICATIONSRanked by the impact factor of the journal

ATP-Binding Cassette Protein ABCF1 Couples Transcription and Genome Surveillance in Embryonic Stem Cells through Low-Complexity Domain

The authors reported the ATP-binding cassette subfamily F member 1 (ABCF1) as a coactivator for OCT4/SOX2 critical for stem cell self-renewal.
[Science Advances]

Full Article

Inner Nuclear Protein Matrin-3 Coordinates Cell Differentiation by Stabilizing Chromatin Architecture

Destabilization of CCCTC-binding factor and cohesin binding correlated with altered transcription and accelerated differentiation. This association was conserved in ESCs.
[Nature Communications]

Full Article

Gain-of-Function Cardiomyopathic Mutations in RBM20 Rewire Splicing Regulation and Re-Distribute Ribonucleoprotein Granules within Processing Bodies

To understand the mechanism of RBM20-associated dilated cardiomyopathy (DCM), researchers engineered isogenic iPSCs with DCM-associated missense mutations in RBM20 as well as RBM20 knockout iPSCs.
[Nature Communications]

Full Article

Integrated Loss- and Gain-of-Function Screens Define a Core Network Governing Human Embryonic Stem Cell Behavior

Investigators described an integrated genome-scale loss- and gain-of-function screening approach to identify genetic networks governing ESC proliferation and differentiation into the three germ layers.
[Genes & Development]

Abstract

Inherent Genomic Properties Underlie the Epigenomic Heterogeneity of Human Induced Pluripotent Stem Cells

Scientists showed that seven human iPSC lines with variable germline potential exhibited substantial epigenomic heterogeneity, despite their uniform transcriptomes.
[Cell Reports]

Full Article Graphical Abstract

MK2 Promotes Tfcp2l1 Degradation via β-TrCP Ubiquitin Ligase to Regulate Mouse Embryonic Stem Cell Self-Renewal

The authors demonstrated that β-transducin repeat-containing protein (β-TrCP) targeted Tfcp2l1 for ubiquitination and degradation in a mitogen-activated protein kinase-activated protein kinase 2 (MK2)-dependent manner.
[Cell Reports]

Full Article Graphical Abstract

CALR Frameshift Mutations in MPN Patient-Derived iPSCs Accelerate Maturation of Megakaryocytes

Scientists generated patient-derived CALRins5- or CALRdel52-positive iPSCs to establish a myeloproliferative neoplasms disease model for molecular and mechanistic studies.
[Stem Cell Reports]

Full Article Graphical Abstract

Cell Division- and DNA Replication-Free Reprogramming of Somatic Nuclei for Embryonic Transcription

Researchers showed that somatic nuclei transferred into mouse four-cell embryos arrested at the G2/M phase underwent reprogramming toward the embryonic state.
[iScience]

Full Article Graphical Abstract

The Specialized Mitotic Behavior of Human Embryonic Stem Cells

Investigators established a cell line of human ESCs stably expressing GFP-α-tubulin and mCherry-H2B by lentiviral infection to analyze and visualize mitosis in detail.
[Cell and Tissue Research]

Abstract

Human iPS Cell Derived RPE Strips for Secure Delivery of Graft Cells at a Target Place with Minimal Surgical Invasion

The authors produced a quickly formed human iPSC-retinal pigment epithelium (RPE) strip in as short as 2 days, which showed a potency to partly expand with the correct apical/basal polarity in vivo, although limited in expansion area in the presence of healthy host RPE.
[Scientific Reports]

Full Article

Watch this innovation showcase from ISSCR 2021 on applications of hPSC-derived lung models.

REVIEWS

Induced Pluripotency in the Context of Stem Cell Expansion Bioprocess Development, Optimization, and Manufacturing: A Roadmap to the Clinic

The complexity and volatility of human iPSCs present several bioprocessing challenges, where the goal is to generate a sufficiently large, high-quality, homogeneous population for downstream differentiation — the derivatives of which must retain functional efficacy and meet regulatory safety criteria in application.
[NPJ Regenerative Medicine]

Full Article

Fumarylacetoacetate Hydrolase Gene as a Knockout Target for Hepatic Chimerism and Donor Liver Production

Investigators review proposed mutations in the recipient embryo to disable hepatogenesis and discuss the advantages of using fumarylacetoacetate hydrolase knockouts and other genetic modifications to disable hepatogenesis.
[Stem Cell Reports]

Full Article

INDUSTRY AND POLICY NEWS

Exacis Biotherapeutics Announces Strategic Partnership with CCRM for Specialty Manufacturing of Services and Investment for Development of iPSC-Derived mRNA-Engineered NK Cells

Exacis Biotherapeutics, Inc. announced initiation of a strategic partnership with Toronto-based Centre for Commercialization of Regenerative Medicine (CCRM) for specialty manufacturing services related to the development of Exacis’ innovative, iPSC-derived mRNA-engineered natural killer cell products to treat cancer.
[Exacis Biotherapeutics, Inc. (PR Newswire, Inc.)]

Press Release

FEATURED EVENT

Ribonuclease Recruitment Using a Small Molecule Reduced c9ALS/FTD r(G₄C₂) Repeat Expansion In Vitro and In Vivo ALS Models

ATP-Binding Cassette Protein ABCF1 Couples Transcription and Genome Surveillance in Embryonic Stem Cells through Low-Complexity Domain

Inner Nuclear Protein Matrin-3 Coordinates Cell Differentiation by Stabilizing Chromatin Architecture

Gain-of-Function Cardiomyopathic Mutations in RBM20 Rewire Splicing Regulation and Re-Distribute Ribonucleoprotein Granules within Processing Bodies

Integrated Loss- and Gain-of-Function Screens Define a Core Network Governing Human Embryonic Stem Cell Behavior

Inherent Genomic Properties Underlie the Epigenomic Heterogeneity of Human Induced Pluripotent Stem Cells

MK2 Promotes Tfcp2l1 Degradation via β-TrCP Ubiquitin Ligase to Regulate Mouse Embryonic Stem Cell Self-Renewal

CALR Frameshift Mutations in MPN Patient-Derived iPSCs Accelerate Maturation of Megakaryocytes

Cell Division- and DNA Replication-Free Reprogramming of Somatic Nuclei for Embryonic Transcription

The Specialized Mitotic Behavior of Human Embryonic Stem Cells

Human iPS Cell Derived RPE Strips for Secure Delivery of Graft Cells at a Target Place with Minimal Surgical Invasion

Induced Pluripotency in the Context of Stem Cell Expansion Bioprocess Development, Optimization, and Manufacturing: A Roadmap to the Clinic

Fumarylacetoacetate Hydrolase Gene as a Knockout Target for Hepatic Chimerism and Donor Liver Production

Exacis Biotherapeutics Announces Strategic Partnership with CCRM for Specialty Manufacturing of Services and Investment for Development of iPSC-Derived mRNA-Engineered NK Cells

Cell Bio Virtual 2021

> See All Events

Postdoctoral Fellow – Organoid Development

Scientist – iPSC Derived Cardiac Tissues

Production Manager – Stem Cell and Organoid

Postdoctoral Research Fellow – iPSC Reprogramming

Postdoctoral Researcher – Neurodegenerative Disease Modeling

> See All Jobs