CURRENT PUBLICATIONS Histone Deacetylase 1 (HDAC1), but not HDAC2, Controls Embryonic Stem Cell Differentiation This genetic study of HDAC1 and HDAC2 in embryonic stem cells, which mimic the embryonic epiblast, has identified a unique requirement for HDAC1 in the optimal activity of HDAC1/2 corepressor complexes and cell fate determination during differentiation. [Proc Natl Acad Sci U S A] A Role for Borg5 During Trophectoderm Differentiation Researchers report that differentiation of embryonic stem cells toward trophectoderm is accompanied by enhanced actin protrusion and cell motility that require up-regulation of Borg5. [Stem Cells] The Transcription Factor TCFAP2C/AP-2Gamma Cooperates with CDX2 to Maintain Trophectoderm Formation Researchers report that the transcription factor Tcfap2c is expressed at a high level in the trophectoderm and is able to induce trophoblast fate in embryonic stem cells. [Mol Cell Biol] Activation of the Imprinted Dlk1-Dio3 Region Correlates with Pluripotency Levels of Mouse Stem Cells In this study, investigators identified a conserved imprinted region of the mouse genome, the Dlk1-Dio3 region, which was activated in fully pluripotent mouse stem cells but repressed in partially pluripotent cells. [J Biol Chem] Repression of Retrotransposal Elements in Mouse Embryonic Stem Cells is Primarily Mediated by DNA Methylation-Independent Mechanism To determine whether intracisternal A-Particles (IAP) is also repressed in pluripotent stem cells by DNA methylation, researchers examined IAP expression in demethylated mouse embryonic stem cells and epiblast-derived stem cells. [J Biol Chem] Variation in Hematopoietic Potential of Induced Pluripotent Stem Cell Lines Researchers compare differentiation capacity of six induced pluripotent stem lines into mesodermal cells and hematopoietic cells through embryoid body formation. [Stem Cell Rev] Alternative Sources of Pluripotent Stem Cells: Ethical and Scientific Issues Revisited Ethically uncompromised stem cells, such as those derived by direct reprogramming or from germ-cell precursors are likely to yield important advances in stem cell research, and move the field rapidly towards clinical applications. [Stem Cells Dev] Rederivation of Transgenic Mice from Induced Pluripotent Stem Cells Derived from Frozen Tissue This study provides conclusive evidence that, as a practical matter, frozen tails and fibroblasts can be used as an effective and reliable alternative to frozen germplasm and embryonic stem cells for the storage, maintenance, and distribution of genetically-altered mutant mice. [Transgenic Res] Derivation and Long-Term Culture of Human Parthenogenetic Embryonic Stem Cells Using Human Foreskin Feeders This study indicates that homologous human fibroblasts can be used as feeder cells to support not only the propagation, but also the derivation of embryonic stem cells, and this should facilitate studies of therapeutic cloning for research and clinical applications. [J Assist Reprod Genet] Preimplantation Mouse Embryo Selection Guided by Light-Induced Dielectrophoresis Researchers present a method that exploits the scaling electrical properties of pre-transfer embryos to quantitatively discern embryo developmental maturity using light-induced dielectrophoresis. [PLoS One]
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