CURRENT PUBLICATIONS (Ranked by Impact Factor of the Journal) Modeling the Long QT Syndrome with Induced Pluripotent Stem Cells Researchers report the development of a patient/disease-specific human induced pluripotent stem cell (iPSC) line from a patient with type-2 long QT syndrome (which is due to the A614V missense mutation in the KCNH2 gene). The generated iPSCs were coaxed to differentiate into the cardiac lineage. [Nature] Efficient Human iPS Cell Derivation by a Non-Integrating Plasmid from Blood Cells with Unique Epigenetic and Gene Expression Signatures The analysis suggested that newborn cord blood and adult peripheral blood mononuclear cells (MNCs) display unique signatures that are closer to induced pluripotent stem cells (iPSCs) and human embryonic stem cells (ESCs) than age-matched fibroblasts to iPSCs/ESCs, thus making blood MNCs an attractive cell choice for the generation of integration-free iPSCs. [Cell Res] Myod Gene Suppression by Oct4 Is Required for Reprogramming in Myoblasts to Produce Induced Pluripotent Stem Cells Results indicate that suppression of MyoD gene expression by Oct4 is required for the initial reprogramming step in the development of induced pluripotent stem cells (iPSCs) from myoblasts. This data suggests that the skeletal muscle system provides a well-defined differentiation model to further elaborate on the effects of iPSC reprogramming in somatic cells. [Stem Cells] Germline Competency of Parthenogenetic Embryonic Stem Cells from Immature Oocytes of Adult Mouse Ovary Researchers report efficient generation of germline competent parthenogenetic embryonic stem cell (pESC) lines (named as IVM pESCs) from parthenogenetic embryos developed from immature oocytes of adult mouse ovaries following in vitro maturation (IVM) and artificial activation. [Hum Mol Genet] Smad-Interacting Protein-1 and MicroRNA 200 Family Define a Nitric Oxide-Dependent Molecular Circuitry Involved in Embryonic Stem Cell Mesendoderm Differentiation The results are the first demonstrating that the microRNA 200 family and Smad-interacting protein-1 (Sip1/ZEB2) transcription factor are regulated by nitric oxide, indicating an unprecedented molecular circuitry important for telomerase regulation and early differentiation of mES. [Arterioscler Thromb Vasc Biol] Generation of Induced Pluripotent Stem Cell Lines from Friedreich Ataxia Patients Researchers demonstrate that following in vitro differentiation the patient-derived induced pluripotent stem (FA-iPS) cells give rise to the two cell types primarily affected in Friedreich ataxia (FRDA), peripheral neurons and cardiomyocytes. The FA-iPS cell lines have the potential to provide valuable models to study the cellular pathology of FRDA and to develop high-throughput drug screening assays. [Stem Cell Rev Rep] Multi-Scale Biomimetic Topography for the Alignment of Neonatal and Embryonic Stem Cell-Derived Heart Cells Researchers present a tunable culture platform comprised of biomimetic ‘wrinkles’ that simulate the heart’s complex anisotropic and multi-scale architecture for facile and robust cardiac cell alignment. They demonstrate the cellular and sub-cellular alignment of both neonatal mouse cardiomyocytes as well as those derived from human embryonic stem cells. [Tissue Eng Part C Methods] Functional Comparison and Expression Profiling of Human Induced Pluripotent Stem Cell- and Embryonic Stem Cell-Derived Endothelial Cells The analysis demonstrates that gene expression variation of human induced pluripotent stem cell (hiPSC)- endothelial cells (ECs) and human embryonic stem cell (hESC)-ECs contributes significantly to biological differences between hiPSC-ECs and hESC-ECs, as well as the “distances” among hiPSCs, hESCs, hiPSC-ECs, hESC-ECs, and human umbilical vein endothelial cells. [Stem Cells Dev] Human Feeder Cells Can Support the Undifferentiated Growth of Human and Mouse Embryonic Stem Cells Using Their Own Basic Fibroblast Growth Factors Human feeder cells are able to support the undifferentiated growth of human and mouse embryonic stem cells (ESCs) via bFGF synthesis. Furthermore a bFGF-dependent pathway might be crucial for maintaining the undifferentiated characteristics of mouse as well as human ESCs. [Stem Cells Dev] Pluripotency Maintenance in Mouse Somatic Cell Nuclear Transfer Embryos and Its Improvement by Treatment with the Histone Deacetylase Inhibitor TSA Researchers followed the fate of the pluripotent cells within somatic cell nuclear transfer (SCNT) embryos, from the late blastocyst to the early epiblast prior to gastrulation. The data show a delay in development correlated with a defect in forming and maintaining a correct number of Oct4 expressing ICM and epiblast cells in SCNT embryos. [Cell Reprogram]
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