Role for Dpy-30 in ES Cell-Fate Specification by Regulation of H3K4 Methylation Within Bivalent Domains Results strongly indicate an essential functional role for Dpy-30 and SET1/MLL complex-mediated H3K4 methylation, as a component of the bivalent mark, at developmental genes during the embryonic stem (ES) cell fate transitions. [Cell] Genomic Instability in Induced Stem Cells Comparative genomic hybridization analysis of stem cells derived by the present protocols revealed the presence of genomic deletions and amplifications, whose signature was suggestive of oncogene-induced DNA replication stress. The genomic aberrations were to a significant degree dependent on c-Myc expression and their presence could explain why p53 inactivation facilitates stem cell reprogramming. [Cell Death Differ] The Use of Leukemia Inhibitory Factor Immobilized on Virus-Derived Polyhedra to Support the Proliferation of Mouse Embryonic and Induced Pluripotent Stem Cells Human leukemia inhibitory factor (LIF) was immobilized into insect virus-derived microcrystals (polyhedra) to generate LIF polyhedra that can slowly release LIF into embryonic stem (ES) cell culture media and thus maintain ES cells in an undifferentiated state. [Biomaterials] Fezf2 Regulates Telencephalic Precursor Differentiation from Mouse Embryonic Stem Cells Data suggest that forebrain embryonic zinc finger like (Fezf2) controls the specification of telencephalic progenitors from mouse embryonic stem cells through differentially regulating the expression of rostrocaudal and dorsoventral patterning genes. [Cereb Cortex] Mismatch and Base Excision Repair Proficiency in Murine Embryonic Stem Cells Data are consistent with the suggestion that embryonic stem cells have multiple mechanisms, including highly active mismatch repair and base-excision repair that preserve genetic integrity and minimize the accumulation of mutations. [DNA Repair] Fibroblasts Derived from Human Embryonic Stem Cells Direct Development and Repair of 3D Human Skin Equivalents Findings demonstrate that human embryonic stem (hES)-derived cells could be directed to specified and alternative mesenchymal cell fates whose function could be distinguished in engineered human skin equivalents (HSEs). Characterization of hES-derived mesenchymal cells in 3D, engineered HSEs demonstrates the utility of this tissue platform to predict the functional properties of hES-derived fibroblasts before their therapeutic transplantation. [Stem Cell Res Ther] Bioluminescence Reporter Gene Imaging Characterize Human Embryonic Stem Cell-Derived Teratoma Formation Human embryonic stem (hES) cells were stably transduced with a double fusion reporter gene consisting of firefly luciferase and enhanced green fluorescent protein. Following bioluminescence imaging and histology, researchers demonstrated that engraftment of hES cells was followed by dramatically increasing signaling and led to teratoma formation confirmed by histology. [J Cell Biochem] Effect of Melatonin on mRNA Expressions of Transcription Factors in Murine Embryonic Stem Cells Melatonin may affect Akt activation, but does not influence the mRNA expressions of Oct4, REX-1, Sox2, or Zfp206, suggesting that melatonin may stimulate proliferation of ES-E14TG2a cells via Akt phosphorylation. [Brain Res] |