Recapitulation of Premature Aging with iPSCs from Hutchinson-Gilford Progeria Syndrome Researchers report the generation of induced pluripotent stem cells (iPSCs) from fibroblasts obtained from patients with Hutchinson-Gilford progeria syndrome. [Nature] Endogenous Retroviruses and Neighboring Genes Are Coordinately Repressed by LSD1/KDM1A Findings suggest that ancient retroviral insertions were used to co-opt regulatory sequences targeted by KDM1A for epigenetic silencing of cell fate genes during early mammalian embryonic development. [Genes Dev] Genome-Wide Analysis of Target Genes Regulated by HoxB4 in Hematopoietic Stem and Progenitor Cells Developing from Embryonic Stem Cells Investigators performed a genome-wide analysis of HoxB4 and global gene expression profiling using embryonic stem cell-derived hematopoietic stem/progenitor cells. [Blood] In Vitro Generation of HSC-Like Cells from Murine ESCs/iPSCs by Enforced Expression of LIM-Homeobox Transcription Factor Lhx2 Results demonstrate that ectopic expression of Lhx2 confers an in vivo engrafting capacity to embryonic stem cell (ESC)/induced pluripotent stem cell (iPSC)-derived hematopoietic cells and in vivo behavior of iPSC-derived hematopoietic cells is almost identical to that of ESC-derived cells. [Blood] Human-Induced Pluripotent Stem Cells are Capable of B-Cell Lymphopoiesis Researchers have been able to demonstrate for the first time that human induced pluripotent stem cells are able to undergo hematopoiesis that contributes to the B cell lymphoid lineage. [Blood] Promyelocytic Leukemia Protein in Retinoic Acid-Induced Chromatin Remodeling of Oct4 Gene Promoter This study demonstrates a functional role for Pml in maintaining a specific open chromatin conformation of the Oct4 promoter region for its constant expression in stem cells; and illustrates the mechanism underlying RA-induced chromatin remodeling of Oct4 gene in differentiating cells, in which Pml plays a critical role. [Stem Cells] Multipotent Adult Germline Stem Cells and Embryonic Stem Cells Functional Proteomics Revealed an Important Role of Eukaryotic Initiation Factor 5A (Eif5a) in Stem Cell Differentiation In order to highlight the protein profile changes associated with stem cell differentiation, retinoic acid treated mouse stem cells (multipotent adult germline stem cells and embryonic stem cells) were compared to non-treated stem cells. [J Proteome Res] Collagen I Regulates the Self-Renewal of Mouse Embryonic Stem Cells Through Alpha2Beta1 Integrin- and DDR1-Dependent Bmi-1 Results indicate that collagen I stimulates the self-renewal of mouse embryonic stem cells mediated by Bmi-1 through alpha2beta1 integrin-dependent ILK, Notch, Gli-1, and DDR1-dependent Ras, PI3K/Akt, and ERK. [J Cell Physiol] L3MBTL1 Deficiency Directs the Differentiation of Human Embryonic Stem Cells Towards Trophectoderm Data demonstrate that depletion of L3MBTL1 does not affect human embryonic stem cell self-renewal, rather, it enhances differentiation towards extra-embryonic trophoblast tissues. [Stem Cells Dev] Isolation and Characterization of Node/Notochord-Like Cells from Mouse Embryonic Stem Cells Researchers used a targeted Noto-GFP reporter to isolate and characterize node/notochord-like cells derived from mouse embryonic stem cells. [Stem Cells Dev] The Amino Acid Transporter SNAT2 Mediates L-Proline Induced Differentiation of ES Cells Data reveal an unexpected role for amino acid uptake and the amino acid transporter SNAT2 in regulation of pluripotent cells in culture, and provides a number of specific, inexpensive and non-toxic culture additives with the potential to improve the quality of embryonic stem (ES) cell culture. [Am J Physiol Cell Physiol] Retinoid-Independent Motor Neurogenesis from Human Embryonic Stem Cells Reveals a Medial Columnar Ground State Researchers investigated the necessity for retinoic acid in motor neurogenesis from human embryonic stem cells. [Nat Commun] Induced Pluripotent Stem Cell Lines Derived from Equine Fibroblasts Scientists report the generation of induced pluripotent stem cells from equine fibroblasts using a piggyBac transposon-based method to deliver transgenes containing the reprogramming factors Oct4, Sox2, Klf4 and c-Myc, expressed in a temporally regulated fashion. [Stem Cell Rev] |