Human iPS-Derived Retinal Pigment Epithelium (RPE) Cells Exhibit Ion Transport, Membrane Potential, Polarized VEGF Secretion and Gene Expression Pattern Similar to Native RPE Researchers show for the first time that RPE generated from human induced pluripotent stem (iPS) cells under defined conditions exhibit ion transport, membrane potential, polarized VEGF secretion and gene expression profile similar to those of native RPE. [Stem Cells] Rcor2 is a Subunit of the LSD1 Complex That Regulates ES Cell Property and Substitutes for Sox2 in Reprogramming Somatic Cells to Pluripotency Researchers report that Rcor2 is predominantly expressed in ES cells and forms a complex with LSD1 and facilitates its nucleosomal demethylation activity. [Stem Cells] Three-Dimensional Growth of iPS Cell-Derived Smooth Muscle Cells on Nanofibrous Scaffolds Data suggest that induced pluripotent stem (iPS) cell-derived smooth muscle cell s can be an important cell source for personalized vascular tissue engineering applications. [Biomaterials] Eya1 Controls Cell Polarity, Spindle Orientation, Cell Fate and Notch Signaling in Distal Embryonic Lung Epithelium Findings uncover novel functions for Eya1 as a crucial regulator of the complex behavior of distal embryonic lung epithelium. [Development] Generation of Dendritic Cells and Macrophages from Human Induced Pluripotent Stem Cells Aiming at Cell Therapy Researchers demonstrated the possibility of application of iPS cell-derived dendritic cells and macrophages to cell therapy. [Gene Ther] A Defined, Feeder-Free, Serum-Free System to Generate In Vitro Hematopoietic Progenitors and Differentiated Blood Cells from hESCs and hiPSCs Researchers showed that a single matrix protein in combination with growth factors and a hypoxic environment is sufficient to generate from pluripotent cells hematopoietic progenitors capable of differentiating further in mature cell types of different lineages of the blood system. [PLoS ONE] Embryonic Stem Cell-Derived L1 Overexpressing Neural Aggregates Enhance Recovery After Spinal Cord Injury in Mice Mice transplanted with L1 overexpressing embryonic stem cell-derived neural aggregates (SENAs) showed improved locomotor function when compared to mice injected with wild-type SENAs. [PLoS ONE] Human Embryonic Stem Cell-Derived Mesenchymal Stem Cells as Cellular Delivery Vehicles for Prodrug Gene Therapy of Glioblastoma Findings suggest human embryonic stem cell-derived mesenchymal stem cells as a viable and attractive alternative for large scale derivation of targeting vehicles for cancer therapy. [Hum Gene Ther] Generation of Red Blood Cells from Human Induced Pluripotent Stem Cells Researchers showed that fibroblast-derived transgenic human induced pluripotent stem cells (hiPSCs) generated using lentivirus-based vectors and transgene-free hiPSCs generated using episomal vectors can be differentiated into red blood cells with an efficiency similar to that of H1 human embryonic stem cells. [Stem Cells Dev] Embryonic Stem Cell-Derived Neurons Are a Novel, Highly Sensitive Tissue Culture Platform for Botulinum Research Hypothesizing that embryonic stem cell-derived neurons (ESNs) might comprise a novel platform to investigate the neurotoxicology of botulinum neurotoxins (BoNTs), researchers evaluated the susceptibility of ESNs to BoNT/A and BoNT/E using molecular and functional assays. [Biochem Biophys Res Commun] Rational Optimization of Reprogramming Culture Conditions for the Generation of Induced Pluripotent Stem Cells with Ultra-High Efficiency and Fast Kinetics Researchers show that reprogramming can be improved and dramatically accelerated by optimizing culture conditions. [Cell Res] |