Multiple Targets of miR-302 and miR-372 Promote Reprogramming of Human Fibroblasts to Induced Pluripotent Stem Cells Researchers show that the human embryonic stem cell-specific cell cycle-regulating miRNA orthologs hsa-miR-302b and hsa-miR-372 promote human somatic cell reprogramming. [Nat Biotechnol] Incomplete DNA Methylation Underlies a Transcriptional Memory of Somatic Cells in Human iPS Cells Researchers carried out a systematic comparison of human induced pluripotent stem (iPS) cells generated from hepatocytes (representative of endoderm), skin fibroblasts (mesoderm) and melanocytes (ectoderm). All low-passage iPS cells analyzed retain a transcriptional memory of the original cells. [Nat Cell Biol] Conserved, Developmentally Regulated Mechanism Couples Chromosomal Looping and Heterochromatin Barrier Activity at the Homeobox Gene A Locus Using mouse embryonic stem cells in both a pluripotent and differentiated neuronal progenitor state, researchers determined that embryonic stem cell pluripotency factor OCT4 antagonizes cohesin loading at the CTCF binding site. [Proc Natl Acad Sci U S A] Single Transcription Factor Reprogramming of Hair Follicle Dermal Papilla Cells to Induced Pluripotent Stem Cells Researchers exploit that dermal papilla cells from hair follicles in the skin express all but one reprogramming factors to show that these accessible cells can be reprogrammed into induced pluripotent stem cells with the single transcription factor Oct4 and without further manipulation. [Stem Cells] Reprogramming of Postnatal Neurons into Induced Pluripotent Stem Cells by Defined Factors Findings indicate that terminally differentiated postnatal neurons are able to acquire the pluripotent state by direct epigenetic reprogramming, and this process is made more efficient through the suppression of lineage specific gene expression. [Stem Cells] Critical-Size Calvarial Bone Defects Healing in a Mouse Model with Silk Scaffolds and SATB2-Modified iPSCs The results demonstrate that SATB2 facilitates the differentiation of induced pluripotent stem cells (iPSCs) towards osteoblast-lineage cells by repressing HoxA2 and augmenting the functions of the osteoblast determinants Runx2, bone sialoprotein and osteocalcin. [Biomaterials] ID1 and ID3 Represent Conserved Negative Regulators of Human Embryonic and Induced Pluripotent Stem Cell Hematopoiesis The study uniquely identifies ID1 and ID3 as negative regulators of the human pluripotent stem cell-hematopoietic transition from a hemogenic to a committed hematopoietic fate, and demonstrates that this is conserved between human embryonic stem cells and human induced pluripotent stem cells. [J Cell Sci] Prion Protein Expression Regulates Embryonic Stem Cell Pluripotency and Differentiation Findings provide strong evidence for a relationship between Prnp and several key pluripotency genes and attribute Prnp a crucial role in regulating self-renewal/differentiation status of embryonic stem cells, confirming the participation of cellular prion protein (PRNP) during early embryogenesis. [PLoS ONE] Generation of LIF and bFGF-Dependent Induced Pluripotent Stem Cells from Canine Adult Somatic Cells Researchers report the generation of canine induced pluripotent stem cells from canine adult fibroblasts, which they accomplished by introducing human OCT4, SOX2, c-MYC, and KLF4. [Stem Cells Dev] E-Cadherin-Expressing Feeder Cells Promote Neural Lineage Restriction of Human Embryonic Stem Cells Researchers have rapidly and efficiently differentiated human embryonic stem cells (hESCs) into neural stem cells by presenting the cell adhesion molecule, E-cadherin, to undifferentiated hESCs via E-cadherin transfected fibroblast monolayers. [Stem Cells Dev] |