The Influence of Scaffold Elasticity on Germ Layer Specification of Human Embryonic Stem Cells Results suggest that three dimensional scaffolds can recapitulate the mechanical stimuli required for directing human embryonic stem cell (hESC) differentiation and that these stimuli can play a significant role in determining hESC fate. [Biomaterials] Abstract Two-Phase Analysis of Molecular Pathways Underlying iPS Cell Induction Here investigators have synchronized induced pluripotent stem (iPS) cell reprogramming process by a two-phase induction: an initial stable intermediate phase following transduction with Oct4, Klf4, and c-Myc, and a final iPS cell phase following overexpression of Sox2. [Stem Cells] Abstract Changes in Glycosphingolipid Composition During Differentiation of Human Embryonic Stem Cells to Ectodermal or Endodermal Lineages In the present study, to further clarify if alterations in glycosphingolipids (GSL) patterns are correlated with lineage-specific differentiation of human embryonic stem cells (hESCs), scientists analyzed changes in GSLs as hESCs differentiated into neural progenitors or endodermal cells by MALDI-MS and MS/MS analyses. [Stem Cells] Abstract L1 Cell Adhesion Molecule, a Novel Surface Molecule of Human Embryonic Stem Cells, Is Essential for Self-Renewal and Pluripotency Here researchers generated monoclonal antibodies that specifically bind to undifferentiated human embryonic stem cells but not to mouse embryonic stem cells. [Stem Cells] Abstract Alternative Splicing Produces Nanog Protein Variants with Different Capacities for Self-Renewal and Pluripotency in Embryonic Stem Cells Results reveal for the first time an expanded Nanog protein coding capacity. Investigators further reveal that a short region of the N-terminal domain and a single phosphorylatable Ser-2 is essential for the maintenance of self-renewal and pluripotency, demonstrating that this region of the protein is highly regulated. [J Biol Chem] Abstract NPR-C Protects Embryonic Stem Cells from Apoptosis by Regulating p53 Levels Here, investigators report a novel role for natriuretic peptide receptor-C (NPR-C) in the survival of murine embryonic stem cells. [Stem Cells Dev] Abstract A Simple and Economical Route to Generate Functional Hepatocyte-Like Cells from hESCs and Their Application in Evaluating Alcohol Induced Liver Damage Here scientists report a simple two-step protocol employing conditioned medium from human hepatocellular carcinoma cell line, HepG2, to generate functional hepatocyte-like cells from human embryonic stem cells (hESCs). [J Cell Biochem] Abstract Comparative Study of Myocytes from Normal and Mdx Mice iPS Cells In this study, researchers try to determine whether there are different characteristics of myocytes from mdx induced pluripotent stem cells (iPS cells) and C57BL/10 iPS cells. [J Cell Biochem] Abstract A Gene Expression—Based Screening System for Compounds Influencing Differentiation of Mouse Embryonic Stem Cells In this study, to classify a number of compounds, the authors established a gene expression–based screening system using mouse embryonic stem cells that monitored multiple parameters. [J Biomol Screen] Abstract |