Single-Cell Expression Analyses during Cellular Reprogramming Reveal an Early Stochastic and a Late Hierarchic Phase During cellular reprogramming, only a small fraction of cells become induced pluripotent stem cells. Previous analyses of gene expression during reprogramming were based on populations of cells, impeding single-cell level identification of reprogramming events. Here, researchers utilized two gene expression technologies to profile 48 genes in single cells at various stages during the reprogramming process. [Cell] Abstract | Press Release | Graphical Abstract Dynamic and Coordinated Epigenetic Regulation of Developmental Transitions in the Cardiac Lineage Scientists interrogated the transcriptome and several histone modifications across the genome during defined stages of cardiac differentiation. They found distinct chromatin patterns that are coordinated with stage-specific expression of functionally related genes, including many human disease-associated genes. [Cell] Abstract | Graphical Abstract Epigenomic Annotation of Enhancers Predicts Transcriptional Regulators of Human Neural Crest To annotate and characterize cis-regulatory elements utilized by the human neural crest cells, scientists coupled a hESC differentiation model with genome-wide profiling of histone modifications and of coactivator and transcription factor occupancy. [Cell Stem Cell] Abstract | Graphical Abstract Directed Differentiation of Human Pluripotent Stem Cells to Cerebral Cortex Neurons and Neural Networks The authors provide a detailed protocol for directing the differentiation of human embryonic stem cells and induced pluripotent stem cells to all classes of cortical projection neurons. [Nat Protoc] Abstract Identification of a Specific Reprogramming-Associated Epigenetic Signature in Human Induced Pluripotent Stem Cells Researchers characterized the epigenomic integrity of 17 human induced pluripotent stem cell (hiPSC) lines derived from six different cell types with varied reprogramming efficiencies. They demonstrated that epigenetic aberrations are a general feature of the hiPSC state and are independent of the somatic cell source. [Proc Natl Acad Sci USA] Abstract | Press Release Zfp281 Mediates Nanog Autorepression through Recruitment of the NuRD Complex and Inhibits Somatic Cell Reprogramming Scientists reported that Nanog is subjected to a negative autoregulatory mechanism, i.e., autorepression, in embryonic stem cells, and that such autorepression requires the coordinated action of the Nanog partner and transcriptional repressor Zfp281. [Proc Natl Acad Sci USA] Abstract Human Pluripotent Stem Cell-Derived Mesenchymal Stem Cells Prevent Allergic Airway Inflammation in Mice Investigators developed a mouse model of ovalbumin-induced allergic inflammation in both the upper and lower airways and evaluated the effects of the systemic administration of human-induced pluripotent stem cell-derived mesenchymal stem cells (MSCs) and bone marrow-derived MSCs on allergic inflammation. [Stem Cells] Abstract Endogenous Wnt Signaling in Human Embryonic Stem Cells Generates an Equilibrium of Distinct Lineage-Specified Progenitors Scientists showed using Wnt reporter human embryonic stem cell (hESC) lines that the cells are heterogeneous with respect to endogenous Wnt signaling activity. Moreover, the level of Wnt signaling activity in individual cells correlates with differences in clonogenic potential and lineage-specific differentiation propensity. [Nat Commun] Abstract Myoblasts Derived from Normal hESCs and Dystrophic hiPSCs Efficiently Fuse with Existing Muscle Fibers following Transplantation The authors developed a two-step procedure to differentiate human embryonic stem cells (hESCs) and dystrophic human-induced pluripotent stem cells (hiPSCs) in myogenic cells. When transplanted in the muscle of Rag/mdx mice, these cells participated in muscle regeneration by fusing very well with existing muscle fibers. [Mol Ther] Full Article Feeder-Free Derivation of Human Induced Pluripotent Stem Cells with Messenger RNA Researchers accelerated the messenger RNA reprogramming process through stepwise optimization of the reprogramming factor cocktail and leveraged these kinetic gains to establish a feeder-free, xeno-free protocol which slashes the time, cost and effort involved in induced pluripotent stem cells derivation. [Sci Rep] Full Article | Press Release |