Long-Term Maintenance of Mouse Embryonic Stem Cell Pluripotency by Manipulating Integrin Signaling within 3D Scaffolds without Active Stat3 Researchers engineered an acellular biomimetic microenvironment to regulate stem cell fate and applied it to maintain mouse embryonic stem cell self-renewal. [Biomaterials] Abstract Targeted Proteomics of the Secretory Pathway Reveals the Secretome of Mouse Embryonic Fibroblasts and Human Embryonic Stem Cells Investigators showed that targeted proteomics of secretory pathway organelles is a powerful alternate approach to interrogate the cellular secretome. They developed procedures to obtain subcellular fractions from mouse embryonic fibroblasts and human embryonic stem cells that are enriched in secretory pathway organelles, while ensuring retention of the secretory cargo. [Mol Cell Proteomics] Abstract | Full Article | Press Release A Kinase Inhibitor Screen Identifies Small-Molecule Enhancers of Reprogramming and iPS Cell Generation Researchers performed an inhibitor screen to identify kinases that enhance, or present a barrier to, reprogramming. In particular, inhibitors of p38, inositol trisphosphate 3-kinase, and Aurora A kinase potently enhance induced pluripotent stem cell (iPSC) generation, and iPSCs derived from inhibitor-treated somatic cells are capable of reaching a fully reprogrammed state. [Nat Commun] Abstract | Press Release Matrix Bound Heparan Sulfate Is Essential for the Growth and Pluripotency of Human Embryonic Stem Cells Data suggested that matrix bound heparan sulfate is required for the growth and pluripotency of human embryonic stem cells and that ethanol-fixed murine embryonic fibroblasts may be used as a ‘live cell’-free substrate for stem cells. [Glycobiology] Abstract Wnt/Beta-Catenin Signaling in Embryonic Stem Cell Converted Tumor Cells Researchers aimed to obtain embryonic stem cells-converted tumor cell lines and to investigate the mechanism of malignancy in pluripotent stem cells. [J Transl Med] Abstract | Full Article Inducing Endoderm Differentiation by Modulating Mechanical Properties of Soft Substrates The authors reported how the cellular mechanical microenvironment of soft substrates affects the differentiation and phenotypic commitment of embryonic stem cells. [J Tissue Eng Regen Med] Abstract Gene-Expression Analysis Reveals that Embryonic Stem Cells Cultured Under Osteogenic Conditions Produce Mineral Non-Specifically Compared to Marrow Stromal Cells or Osteoblasts Researchers related the expression of osteogenic and chondrogenic genes in cultures of murine embryonic stem cells, marrow stromal cells and calvarial osteoblasts cultured under osteogenic conditions to the biochemical composition and quantity of mineral formed. [Eur Cell Mater] Abstract High-Throughput Screening for Bioactive Molecules Using Primary Cell Culture of Transgenic Zebrafish Embryos Scientists reported a high-throughput approach for screening small molecules that regulate cell differentiation using lineage-specific green fluorescent protein transgenic zebrafish embryonic cells. 23 molecules were confirmed to enhance angiogenesis, and 11 of them were validated to promote the proliferation of mammalian human umbilical vascular endothelial cells and induce Flk1+ cells from murine embryonic stem cells. [Cell Rep] Abstract |