Genome-Wide Nucleosome Positioning during Embryonic Stem Cell Development Investigators determined genome-wide nucleosome occupancies in mouse embryonic stem cells and their neural progenitor and embryonic fibroblast counterparts to assess features associated with nucleosome positioning during lineage commitment. [Nat Struct Mol Biol] Abstract Generation of Integration-Free Human Induced Pluripotent Stem Cells from Postnatal Blood Mononuclear Cells by Plasmid Vector Expression Researchers described a detailed, validated protocol for effective generation of integration-free human induced pluripotent stem cells from blood mononuclear cells by plasmid vectors. [Nat Protoc] Abstract Identification of Key Regulatory Pathways of Myeloid Differentiation Using an mESC-Based Karyotypically Normal Cell Model The authors showed that myeloid progenitors can be derived from embryonic stem cells (ESCs), immortalized, and applied to the study of the mechanisms underlying myeloid differentiation. [Blood] Abstract In Vivo Maturation of Functional Renal Organoids Formed from Embryonic Cell Suspensions Producing tissues similar to immature kidneys from simple suspensions of fully dissociated embryonic renal cells is possible in vitro, but glomeruli do not form in the avascular environment. Here, researchers constructed renal organoids from single-cell suspensions derived from E11.5 kidneys and then implanted these organoids below the kidney capsule of a living rat host. [J Am Soc Nephrol] Abstract | Press Release Limited Gene Expression Variation in Human Embryonic Stem Cell and Induced Pluripotent Stem Cell Derived Endothelial Cells Scientists report a differentiation protocol based on embryonic development that consistently yields large numbers of endothelial cells derived from multiple human embryonic stem or induced pluripotent stem cells. [Stem Cells] Abstract | Full Article TGF-Beta-Superfamily Signaling Regulates Embryonic Stem Cell Heterogeneity: Self-Renewal as a Dynamic and Regulated Equilibrium The authors sought to define the activities of two TGF-beta-related signaling pathways, bone morphogenetic protein and Nodal signaling, in modulating mouse embryonic stem cell heterogeneity in undifferentiated culture conditions. [Stem Cells] Abstract Temporal Application of Topography to Increase the Rate of Neural Differentiation from Human Pluripotent Stem Cells Researchers explored the effect of topography on the neural differentiation of human pluripotent stem cells by quantitatively studying the changes in marker expression at a transcript and protein level. [Biomaterials] Abstract Cardiac Progenitors Derived from Reprogrammed Mesenchymal Stem Cells Contribute to Angiomyogenic Repair of the Infarcted Heart Scientists reported efficient reprogramming of multipotent bone marrow mesenchymal stem cells (MSC) to pluripotent status. The resultant MSC derived induced pluripotent stem cells and their derived progenitors effectively repaired the infarcted heart. [Basic Res Cardiol] Abstract | Full Article Human Embryonic Stem Cell-Derived Oligodendrocyte Progenitors Aid in Functional Recovery of Sensory Pathways following Contusive Spinal Cord Injury Studies have already demonstrated cells’ survival following transplantation in spinal cord injury. The authors sought to evaluate survival and potential therapeutic effects of transplanted human embryonic stem cell-derived oligodendrocyte progenitor cells in a contusive injury in rats. [PLoS One] Full Article Gene Expression Signatures of Extracellular Matrix and Growth Factors during Embryonic Stem Cell Differentiation Investigators examined the global dynamic profiles of extracellular matrix and growth factor genes associated with early stages of pluripotent mouse embryonic stem cell differentiation. [PLoS One] Full Article |