Gata4 Blocks Somatic Cell Reprogramming by Directly Repressing Nanog Somatic cells can be reprogrammed to induced-pluripotent stem cells by ectopic expression of the four factors Oct4, Klf4, Sox2 and Myc. Researchers investigated the role of Gata4 in the reprogramming process and present evidence for a negative role of this family of transcription factors in the induction of pluripotency. [Stem Cells] Abstract Self-Organizing Neuroepithelium from Human Pluripotent Stem Cells Facilitates Derivation of Photoreceptors Scientists reported that formation of a transient self-organized neuroepithelium from human embryonic stem cells cultured together with extracellular matrix is sufficient to induce a rapid conversion into retinal progenitors in five days. [Stem Cells] Abstract Neuronopathic Gaucher’s Disease: Induced Pluripotent Stem Cells for Disease Modeling and Testing Chaperone Activity of Small Compounds The authors developed an induced pluripotent stem cell (iPSC) model of neuronopathic Gaucher’s Disease. Dermal fibroblasts of a patient with a P.[LEU444PRO];[GLY202ARG] genotype were transfected with a loxP-flanked polycistronic reprogramming cassette consisting of Oct4, Sox2, Klf4 and c-Myc and iPSC lines derived. [Hum Mol Genet] Abstract A Comparison of Human Cord Blood- and Embryonic Stem Cell-Derived Endothelial Progenitor Cells in the Treatment of Chronic Wounds Researchers directly compared the capacity of human embryonic stem cell-derived endothelial progenitor cells (EPCs) and human cord blood-derived EPCs to benefit wound healing. [Biomaterials] Abstract BMP4 Sufficiency to Induce Choroid Plexus Epithelial Fate from Embryonic Stem Cell-Derived Neuroepithelial Progenitors During development, choroid plexus epithelial cells (CPECs) differentiate from preneurogenic neuroepithelial cells and require bone morphogenetic protein (BMP) signaling, but whether BMPs suffice for CPEC induction is unknown. Here investigators provided evidence for BMP4 sufficiency to induce CPEC fate from neural progenitors derived from mouse embryonic stem cells. [J Neurosci] Abstract | Press Release In Vitro Modeling of Paraxial Mesodermal Progenitors Derived from Induced Pluripotent Stem Cells Researchers established a protocol for the differentiation of mouse induced pluripotent stem cells into paraxial mesodermal lineages in serum-free culture. [PLoS One] Full Article Chromosome-Wide Regulation of Euchromatin-Specific DNA Cytosine Methylation to 5hmC Conversion in Mouse ES Cells and Female Human Somatic Cells Scientists demonstrated that 5-hydroxymethylcytosine (5hmC) accumulated on euchromatic chromosomal bands that were marked with di- and tri-methylated histone H3 at lysine 4 in mouse embryonic stem (ES) cells. [Chromosome Res] Abstract | Full Article Embryonic Stem Cell-Derived M2-Like Macrophages Delay Cutaneous Wound Healing The authors investigated whether atopical application of ex vivo generated, embryonic stem cell-derived macrophages (ESDM) improve wound healing in mice. ESDM were tested side by side with bone marrow-derived macrophages. [Wound Repair Regen] Abstract Cryopreservation of Pluripotent Stem Cell Aggregates in Defined Protein-Free Formulation Researchers assessed the suitability of cryopreserving intact mouse embryonic stem cell (mESC) aggregates and investigated the effects of aggregate size and the formulation of cryopreservation solution on mESC survival and recovery. [Biotechnol Prog] Abstract Generation of Human Induced Pluripotent Stem Cells from Peripheral Blood Using the Stem Cell Cassette Lentiviral Vector Through the ectopic expression of four transcription factors, Oct4, Klf4, Sox2 and cMyc, human somatic cells can be converted to a pluripotent state, generating so-called induced pluripotent stem cells (iPSCs). Researchers demonstrated a protocol for the generation of human iPSCs from peripheral blood mononuclear cells using a single floxed-excisable lentiviral vector constitutively expressing the four factors. [J Vis Exp] Abstract | Press Release | Video |