Negligible Immunogenicity of Terminally Differentiated Cells Derived from Induced Pluripotent or Embryonic Stem Cells Investigators examined the immunogenicity of differentiated skin and bone marrow tissues derived from mouse induced pluripotent stem cells (iPSCs). To ensure optimal comparison of iPSCs and embryonic stem (ES) cells, they established ten integration-free iPSC and seven ES-cell lines using an inbred mouse strain, C57BL/6. [Nature] Abstract | Press Release Regeneration of Human Tumor Antigen-Specific T Cells from iPSCs Derived from Mature CD8+ T Cells Researchers established iPSCs from mature cytotoxic T cells specific for the melanoma epitope MART-1. When cocultured with OP9/DLL1 cells, these iPSCs efficiently generated TCRß+CD4+CD8+ double positive cells expressing a T cell receptor specific for the MART-1 epitope. Stimulation of the CD8+ T cells with MART-1 antigen-presenting cells led to the secretion of IFNγ, demonstrating their specific reactivity. [Cell Stem Cell] Abstract | Graphical Abstract | Press Release Generation of Rejuvenated Antigen-Specific T Cells by Reprogramming to Pluripotency and Redifferentiation Investigators reprogrammed clonally expanded antigen-specific CD8+ T cells from an HIV-1-infected patient to pluripotency. The T cell-derived induced pluripotent stem cells were then redifferentiated into CD8+ T cells that had a high proliferative capacity and elongated telomeres. These rejuvenated cells possessed antigen-specific killing activity and exhibited T cell receptor gene-rearrangement patterns identical to those of the original T cell clone from the patient. [Cell Stem Cell] Abstract | Graphical Abstract | Press Release Gli3-Mediated Hedgehog Inhibition in Human Pluripotent Stem Cells Initiates and Augments Developmental Programming of Adult Hematopoiesis Using human pluripotent stem cells (hPSCs), researchers revealed that reduction of Hh signaling correlates to developmental progression of hematopoiesis throughout human ontogeny. Both chemical- and gene targeting-mediated inactivation of Hh signaling augmented hematopoietic fate, and initiated transitions from embryonic to adult hematopoiesis, as measured by globin regulation in hPSCs. [Blood] Abstract Directed Differentiation of Human Pluripotent Cells to Neural Crest Stem Cells This protocol describes in detail how to perform a highly efficient, lineage-specific differentiation of human pluripotent cells to a neural crest stem cell fate. [Nat Protoc] Abstract An Oct4-Sall4-Nanog Network Controls Developmental Progression in the Pre-Implantation Mouse Embryo Researchers present the first systematic investigation of the network in pre-implantation mouse embryos using morpholino-mediated gene knockdowns of key embryonic stem cell factors followed by detailed transcriptome analysis of pooled embryos, single embryos, and individual blastomeres. [Mol Syst Biol] Full Article A Thermoresponsive and Chemically Defined Hydrogel for Long-Term Culture of Human Embryonic Stem Cells Investigators report the identification of a family of chemically defined thermoresponsive synthetic hydrogels based on 2-(diethylamino)ethyl acrylate, which support long-term human embryonic stem cell growth and pluripotency over a period of two to six months. [Nat Commun] Abstract | Press Release Long Interspersed Nucleotide Element-1 Hypomethylation in Folate-Deficient Mouse Embryonic Stem Cells To explore whether folate deficiency directly induced long interspersed nucleotide element-1 (LINE-1) hypomethylation and to analyze the relationship between folate concentration and the LINE-1 methylation level, mouse embryonic stem cells were treated with various concentrations of folate, which was measured by chemiluminescent immunoassay, and the homocysteine content was detected by ELISA. [J Cell Biochem] Abstract |