Direct Conversion of Fibroblasts to Neurons by Reprogramming PTB-Regulated MicroRNA Circuits Researchers report that repression of a single RNA binding polypyrimidine-tract-binding (PTB) protein, which occurs during normal brain development via the action of miR-124, is sufficient to induce trans-differentiation of fibroblasts into functional neurons. [Cell] Abstract | Graphical Abstract | Press Release Selective Elimination of Human Pluripotent Stem Cells by an Oleate Synthesis Inhibitor Discovered in a High-Throughput Screen Investigators performed a high-throughput screen of over 52,000 small molecules and identified 15 pluripotent cell-specific inhibitors (PluriSIns), nine of which share a common structural moiety. The PluriSIns selectively eliminated human pluripotent stem cells while sparing a large array of progenitor and differentiated cells. [Cell Stem Cell] Abstract | Graphical Abstract Timothy Syndrome Is Associated with Activity-Dependent Dendritic Retraction in Rodent and Human Neurons Scientists report that channels with the Timothy syndrome alteration cause activity-dependent dendrite retraction in rat and mouse neurons and in induced pluripotent stem cell-derived neurons from individuals with Timothy syndrome. [Nat Neurosci] Abstract Derivation of Human Embryonic Stem Cells Using a Post-Inner Cell Mass Intermediate Researchers provide a detailed (seven-day) protocol for the culture of the human preimplantation embryos in order to derive the post-inner cell mass intermediate (PICMI). Subsequent identification and cryopreservation of the PICMI are described, in addition to human embryonic stem cell derivation. [Nat Protoc] Abstract Factors Expressed by Murine Embryonic Pancreatic Mesenchyme Enhance Generation of Insulin-Producing Cells from hESCs Researchers identified a set of signaling factors expressed in mouse embryonic mesenchyme during the time when foregut and pancreatic progenitors are specified and tested their activities during in vitro differentiation of human embryonic stem cells (hESCs). [Diabetes] Abstract Canonical Wnt Signaling Induces a Primitive Endoderm Metastable State in Mouse Embryonic Stem Cells To investigate the role of canonical Wnt signaling in pluripotency and lineage specification, researchers expressed Wnt3a in mouse embryonic stem cells (mESCs) and characterized them in growth and differentiation. They found that activated canonical Wnt signaling induced the formation of a reversible metastable primitive endoderm state in mESCs. [Stem Cells] Abstract | Full Article An Oct4-pRB Axis, Controlled by MiR-335, Integrates Stem Cell Self-Renewal and Cell Cycle Control Investigators present a new regulatory circuit that connects miR-335, Oct4 and the Retinoblastoma pathway to control mouse embryonic stem cell self-renewal and differentiation. [Stem Cells] Abstract Human Induced Pluripotent Stem Cell-Derived Ectodermal Precursor Cells Contribute to Hair Follicle Morphogenesis In Vivo Ectodermal precursor cells with the capacity to crosstalk with hair inductive dermal cells were generated from human induced pluripotent stem cells and assessed for hair follicle forming ability in vivo. [J Invest Dermatol] Abstract Endothelial Reconstitution by CD34+ Progenitors Derived from Baboon Embryonic Stem Cells Researchers used a large non-human primate model, the baboon, to establish a step-wise protocol to generate CD34+ endothelial progenitor cells from embryonic stem cells and to demonstrate their reparative effects. [J Cell Mol Med] Abstract | Full Article | Press Release The L-Type Ca2+ Channels Blocker Nifedipine Represses Mesodermal Fate Determination in Murine Embryonic Stem Cells The authors examined the contribution of L-type Ca2+ channels and the effect of nifedipine on the commitment of pluripotent stem cells toward the cardiac lineage in vitro. [PLoS One] Full Article |