Lack of Immune Response to Differentiated Cells Derived from Syngeneic Induced Pluripotent Stem Cells Scientists differentiated mouse induced pluripotent stem cells (iPSCs) into embryoid bodies (EBs) or representative cell types spanning the three embryonic germ layers and assessed their immunogenicity in vitro and after their transplantation into syngeneic recipients. They found no evidence of increased T cell proliferation in vitro, rejection of syngeneic iPSC-derived EBs/tissue-specific cells after transplantation, or an antigen-specific secondary immune response. [Cell Stem Cell] Abstract Impaired Epithelial Differentiation of Induced Pluripotent Stem Cells from Ectodermal Dysplasia-Related Patients Is Rescued by the Small Compound APR-246/PRIMA-1MET Researchers designed a unique cellular model that recapitulated major embryonic defects related to ectrodactyly, ectodermal dysplasia, and cleft lip/palate (EEC) syndrome. Fibroblasts from healthy donors and EEC patients carrying two different point mutations in the DNA binding domain of p63 were reprogrammed into induced pluripotent stem cell lines. [Proc Natl Acad Sci USA] Abstract HAT Cofactor Trrap Maintains Self-Renewal and Restricts Differentiation of Embryonic Stem Cells Investigators showed that conditional deletion of the histone acetyltransferase (HAT) cofactor Trrap in mouse embryonic stem cells triggers unscheduled differentiation associated with loss of histone acetylation, condensation of chromatin into distinct foci (heterochromatization), and uncoupling of H3K4 dimethylation and H3K27 trimethylation. [Stem Cells] Abstract Regulation of L-Threonine Dehydrogenase (TDH) in Somatic Cell Reprogramming Scientists report TDH as a novel regulator of somatic cell reprogramming. Knockdown of TDH inhibits, whereas induction of TDH enhances reprogramming efficiency. [Stem Cells] Abstract Gbx2, a LIF/Stat3 Target, Promotes Reprogramming to and Retention of the Pluripotent Ground State Investigators identified gastrulation brain homeobox 2 (Gbx2) as a leukemia inhibitory factor (LIF)/signal transducer and activator of transcription 3 (Stat3) downstream target that when overexpressed allows long-term expansion of undifferentiated mouse embryonic stem cells in the absence of LIF/Stat3 signaling. [J Cell Sci] Abstract Embryonic Stem Cells Facilitate the Isolation of Persistent Clonal Cardiovascular Progenitor Cell Lines and Leukemia Inhibitor Factor Maintains Their Self-Renewal and Myocardial Differentiation Potential In Vitro Using postnatal murine hearts with a selectable marker as the stem cell source and embryonic stem cells and leukemia inhibitory factor-secreting fibroblasts as a surrogate niche, researchers succeeded in the isolation of stable clonal cardiovascular progenitor cell lines. [Cells Tissues Organs] Abstract Derivation and Isolation of NKX2.1-Positive Basal Forebrain Progenitors from Human Embryonic Stem Cells Investigators report efficient derivation of gamma aminobutyric acid (GABA)ergic progenitors using an established human embryonic stem cell (hESC) reporter line that expresses green fluorescent protein under the control of an endogenous NKX2.1 promoter. GABAergic progenitors were derived from this hESC line by a modified monolayer neural differentiation protocol. [Stem Cells Dev] Abstract The Long-Term Differentiation of Embryonic Stem Cells into Cardiomyocytes: An Indirect Co-Culture Model Mouse embryonic stem cells (ESCs) were cultured in hanging drops to form embryoid bodies (EBs) and treated with 0.1 mmol/L ascorbic acid to induce the differentiation of ESCs into cardiomyocytes (CMs). During time course of ESC differentiation, the percentages of EBs with contracting areas in neonatal CMs co-culture were significantly higher than that without co-culture or in epidermal keratinocytes co-culture. [PLoS One] Full Article BMP and TGF-ß Pathway Mediators Are Critical Upstream Regulators of Wnt Signaling during Midbrain Dopamine Differentiation in Human Pluripotent Stem Cells Scientists report that the transient inhibition of constitutive BMP (pSMADs1,5,8) signaling, either alone or in combination with TGF-ß inhibition (pSMADs2,3), is critically important in the upstream regulation of Wnt1-Lmx1a signaling in midbrain dopamine progenitors. [Dev Biol] Abstract Reprogramming of Fibroblasts from Older Women with Pelvic Floor Disorders Alters Cellular Behavior Associated with Donor Age Investigators report successful derivation of induced pluripotent stem cells from women with pelvic organ prolapse. Older age did not interfere with successful reprogramming. [Stem Cells Transl Med] Abstract |