Kdm2b Maintains Murine Embryonic Stem Cell Status by Recruiting PRC1 Complex to CpG Islands of Developmental Genes Scientists showed that the H3K36-specific histone demethylase Kdm2b is highly expressed in mouse embryonic stem cells (mESCs) and regulated by the pluripotent factors Oct4 and Sox2 directly. Depletion of Kdm2b in mESCs causes de-repression of lineage-specific genes and induces early differentiation. Kdm2b interacts with the core components of polycomb repressive complex 1 (PRC1) and recruits the complex to the CpG islands of early lineage-specific genes. [Nat Cell Biol] Abstract Complete and Unidirectional Conversion of Human Embryonic Stem Cells to Trophoblast by BMP4 Researchers confirmed that human embryonic stem cells (hESC) grown under the standard conditions on a medium conditioned by mouse embryonic fibroblasts in the presence of bone morphogenic protein 4 (BMP4) and absence of FGF2 on a Matrigel substratum rapidly convert to an epithelium that is largely KRT7+ within 48 hours, with minimal expression of mesoderm markers, including T (Brachyury). The experiments demonstrated that the in vitro BMP4/hESC model is valid for studying the emergence and differentiation of trophoblasts. [Proc Natl Acad Sci USA] Abstract | Full Article Mechanistic Insight into the Pathology of Polyalanine Expansion Disorders Revealed by a Mouse Model for X Linked Hypopituitarism Investigators used targeted mutagenesis of embryonic stem (ES) cells to generate mice with a polyalanine expansion mutation in Sox3 that is associated with X-linked Hypopituitarism in humans. By investigating both ES cells and chimeric mice, they showed that endogenous polyalanine expanded SOX3 does not form protein aggregates in vivo but rather is present at dramatically reduced levels within the nucleus of mutant cells. [PLoS Genet] Full Article Oct4 Interaction with Hmgb2 Regulates Akt Signaling and Pluripotency Researchers demonstrated that Oct4 post-translational modifications form a positive feedback loop, which promote Akt activation and interaction with Hmgb2 and the SET Complex. This preserves H3K27me3 modifications in daughter progeny and maintains the pluripotent gene expression signature in mESCs. [Stem Cells] Abstract | Full Article Heterogeneous Nuclear Ribonucleoprotein A2/B1 Regulates the Self-Renewal and Pluripotency of Human Embryonic Stem Cells via the Control of the G1/S Transition Scientists found that heterogeneous nuclear ribonucleoprotein A2/B1 expression was localized to undifferentiated human embryonic stem cells (hESCs), and decreased upon differentiation of hESCs. [Stem Cells] Abstract Delivery of Definable Number of Drug or Growth Factor Loaded Poly(DL-lactic acid-co-glycolic acid) Microparticles within Human Embryonic Stem Cell Derived Aggregates Investigators aimed to create an embryoid body (EB) model with localized drug and/or growth factor delivery directly within the EB. Using poly(DL-lactic acid-co-glycolic acid) microparticles (MPs) with an average diameter of 13 µm, they demonstrated controllable incorporation of defined numbers of MPs within human ES cell derived EBs, down to 1 MP per EB. [J Control Release] Abstract PET Demonstrates Functional Recovery after Transplantation of Induced Pluripotent Stem Cells in a Rat Model of Cerebral Ischemic Injury Researchers aimed to determine the functionality of the transplanted induced pluripotent stem cells and embryonic stem cells in a rat model of cerebral ischemia with use of 18F-FDG small-animal positron emission tomography (PET) imaging. [J Nucl Med] Abstract Embryonic Stem Cell-Based Modeling of Tau Pathology in Human Neurons The authors explored whether human embryonic stem cell-derived neural stem cells can be exploited to study consequences of an overexpression of two normal N-terminal domains, four microtubule-binding domains tau versus pseudohyperphosphorylated tau directly in human neurons. [Am J Pathol] Abstract Induced Pluripotent Stem Cell-Derived Neural Cells Survive and Mature in the Nonhuman Primate Brain Investigators showed that transplanted autologous rhesus monkey induced pluripotent stem cell-derived neural progenitors survive for up to six months and differentiate into neurons, astrocytes, and myelinating oligodendrocytes in the brains of MPTP-induced hemiparkinsonian rhesus monkeys with a minimal presence of inflammatory cells and reactive glia. [Cell Rep] Abstract | Graphical Abstract | Press Release Nonxenogeneic Growth and Retinal Differentiation of Human Induced Pluripotent Stem Cells Researchers tested the ability of human induced pluripotent stem cells (hiPSCs) to give rise to retinal cells under nonxenogeneic conditions. hiPSCs were maintained in traditional, feeder-free, or xeno-free culture conditions, and their ability to differentiate to a retinal fate was tested. [Stem Cells Transl Med] Abstract | Press Release |