Disease-Causing Mitochondrial Heteroplasmy Segregated within Induced Pluripotent Stem Cell Clones Derived from a MELAS Patient Mitochondrial diseases display pathological phenotypes according to the mixture of mutant versus wild-type mitochondrial DNA, known as heteroplasmy. Scientists examined the impact of nuclear reprogramming and clonal isolation of induced pluripotent stem cells on mitochondrial heteroplasmy. [Stem Cells] Abstract Identification and Characterization of a Transient Outward K+ Current in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes The ability to recapitulate mature adult phenotypes is critical to development of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) as models of disease. Researchers examined the characteristics of the transient outward current and its contribution to the hiPSC-CM action potential. [J Mol Cell Cardiol] Abstract Engineered Human Embryonic Stem Cell-Derived Lymphocytes to Study In Vivo Trafficking and Immunotherapy Investigators used both bioluminescent and fluorescent imaging to demonstrate trafficking of human embryonic stem cell-derived natural killer cells to tumors in vivo. [Stem Cells Dev] Abstract Improved Methods for Reprogramming Human Dermal Fibroblasts Using Fluorescence Activated Cell Sorting Researchers described an improved method for the derivation of induced pluripotent stem cell lines using fluorescence activated cell sorting to isolate single cells expressing the cell surface marker signature CD13NEGSSEA4POSTra-1-60POS on day 7-10 after infection. [PLoS One] Full Article | Press Release Neuronal Differentiation of Embryonic Stem Cell Derived Neuronal Progenitors Can Be Regulated by Stretchable Conducting Polymers The authors tuned the electrical and mechanical cues provided to embryonic stem cells during differentiation by precisely straining the conducting polymer-coated, elastomeric-substrate. [Tissue Eng Part A] Abstract The Impact of Enriched Environment and Transplantation of Murine Cortical Embryonic Stem Cells on Recovery from Controlled Cortical Contusion Injury Scientists aimed to determine if post-surgical environmental enrichment impacts the survival, migration, and integration of embryonic stem cells in a rodent model of traumatic brain injury and if the presence of these cells lead to improved outcomes. [Restor Neurol Neurosci] Abstract Identification of Small Molecules that Promote Human Embryonic Stem Cell Self-Renewal Researchers report identification of small molecules, some of which were neurotransmitter antagonists (trimipramine and ethopropazine), which promote long-term human embryonic stem cell self-renewal without basic fibroblast growth factor in the medium. [Biochem Biophys Res Commun] Abstract In Vitro and In Vivo Differentiation of Induced Pluripotent Stem Cells into Male Germ Cells Mouse induced pluripotent stem (miPS) cell line 4.1 has been established via retroviral transfer of human transcription factors Oct4, Sox2, Klf4, and c-Myc; the germline competence of this line has not been determined. Researchers induced the differentiation of miPS-4.1 cells into male germ cells, in vivo and in vitro. [Biochem Biophys Res Commun] Abstract Embryonic Stem Cell-Derived Granulosa Cells Participate in Ovarian Follicle Formation In Vitro and In Vivo Investigators demonstrated that Forkhead box L2-expressing ovarian somatic cells derived in vitro from differentiating embryonic stem cells express granulosa cell markers, actively associate with germ cells in vitro, synthesize steroids, respond to follicle-stimulating hormone, and participate in folliculogenesis in vivo. [Reprod Sci] Abstract C/EBPα Induces Highly Efficient Macrophage Transdifferentiation of B Lymphoma and Leukemia Cell Lines and Impairs Their Tumorigenicity Testing >20 human lymphoma and leukemia B cell lines, researchers found that most can be transdifferentiated at least partially into macrophage-like cells, provided that C/EBPα is expressed at sufficiently high levels. [Cell Rep] Abstract | Full Article | Press Release |