Architectural Protein Subclasses Shape 3D Organization of Genomes during Lineage Commitment Understanding the topological configurations of chromatin may reveal valuable insights into how the genome and epigenome act in concert to control cell fate during development. Researchers generated high-resolution architecture maps across seven genomic loci in embryonic stem cells and neural progenitor cells. [Cell] Abstract | Graphical Abstract Sequential Introduction of Reprogramming Factors Reveals a Time-Sensitive Requirement for Individual Factors and a Sequential EMT-MET Mechanism for Optimal Reprogramming Present practices for reprogramming somatic cells to induced pluripotent stem cells involve simultaneous introduction of reprogramming factors. Investigators reported that a sequential introduction protocol (Oct4-Klf4 first, then c-Myc and finally Sox2) outperforms the simultaneous one. [Nat Cell Biol] Abstract | Press Release Deficits in Human Trisomy 21 iPSCs and Neurons Down syndrome (trisomy 21) is the most common genetic cause of intellectual disability, but the precise molecular mechanisms underlying impaired cognition remain unclear. Elucidation of these mechanisms has been hindered by the lack of a model system that contains full trisomy of chromosome 21 (Ts21) in a human genome that enables normal gene regulation. To overcome this limitation, researchers created Ts21-induced pluripotent stem cells (iPSCs) from two sets of Ts21 human fibroblasts. [Proc Natl Acad Sci USA] Abstract | Full Article | Press Release High Throughput Screening for Inhibitors of Rest in Neural Derivatives of Human Embryonic Stem Cells Reveals a Chemical Compound that Promotes Expression of Neuronal Genes High-throughput screening of a library of 6,984 compounds with luciferase-assay measuring REST activity in neural derivatives of human embryonic stem cells led to identify two benzoimidazole-5-carboxamide derivatives that inhibited REST silencing in a repressor element-1 (RE1)-dependent manner. The most potent compound, X5050, targeted REST degradation, but neither REST expression, RNA splicing nor binding to RE1 sequence. [Stem Cells] Abstract An Epigenetic Signature of Developmental Potential in Neural Stem Cells and Early Neurons Researchers interrogated the epigenome during differentiation of embryonic stem cell (ESC)-derived neural stem cells (NSCs) to immature GABAergic interneurons. They showed that developmental transitions are accompanied by loss of bivalency at many promoters in line with their increasing developmental restriction from pluripotent ESC through multipotent NSC to committed GABAergic interneuron. [Stem Cells] Abstract Three-Dimensional Co-Cultures of Human Endothelial Cells and Embryonic Stem Cell-Derived Pericytes Inside a Microfluidic Device Scientists engineered three-dimensional constructs of vascular tissue inside microchannels by injecting a mixture of human umbilical vein endothelial cells, human embryonic stem cell-derived pericytes and rat tail collagen I into a polydimethylsiloxane microfluidic channel with dimensions 500 μm × 120 μm × 1 cm (w × h × l). [Lab Chip] Abstract The Tumorigenicity of iPS Cells and Their Differentiated Derivates Undifferentiated induced pluripotent stem cells (iPSCs), iPSC derivates from induced differentiation (iPSC-derivates), iPSC-derivated cardiomyocytes were subcutaneously injected into the back of nude mice. Non-invasive bioluminescence imaging was longitudinally performed at day 1, 7, 14 and 28 after transplantation to track the survival and proliferation of transplanted cells. At day 28, mice were killed and grafts were explanted to detect teratoma formation. [J Cell Mol Med] Full Article The Establishment of Mouse Embryonic Stem Cell Cultures on 96-Well Plates for High-Throughput Screening The application of embryonic stem (ES) cells can be a useful tool for drug discovery and toxicology. Researchers suggest the high-throughput screening system based on ES cells. [Mol Cells] Abstract |