ESC & iPSC News Volume 8.25 | Jun 26 2013

ESC & iPSC News 8.25 June 26, 2013

ESC & iPSC News

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Biowire: A Platform for Maturation of Human Pluripotent Stem Cell-Derived Cardiomyocytes
Researchers described a platform that combines three-dimensional cell cultivation with electrical stimulation to mature human pluripotent stem cell-derived cardiac tissues. [Nat Methods] Abstract | Press Release
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PUBLICATIONS (Ranked by impact factor of the journal)
Acetylated Histone H3K56 Interacts with Oct4 to Promote Mouse Embryonic Stem Cell Pluripotency
The authors report that Oct4 interacts with acetylated histone H3K56 (H3K56ac) in mouse ES cell (ESC) nuclear extracts and that perturbing H3K56 acetylation decreases Oct4-H3 binding. Data suggest that H3K56ac plays a central role in binding to Oct4 to promote the pluripotency of ESCs. [Proc Natl Acad Sci USA] Abstract

VGLL4 Is a Novel Regulator of Survival in Human Embryonic Stem Cells
Scientists identified Vestigial-like 4 (VGLL4), a co-transcriptional regulator with no previously described function in human embryonic stem cells (hESCs), as a positive regulator of survival in hESCs. Specifically, VGLL4 overexpression in hESCs significantly decreases cell death in response to dissociation stress. [Stem Cells] Abstract

Hypoxia Induces Re-Entry of Committed Cells into Pluripotency
Investigators showed that hypoxia can govern cell fate decision since hypoxia alone can revert human embryonic stem cell (hESC)- or iPSC-derived differentiated cells back to a stem cell-like state, as evidenced by re-activation of an Oct4-promoter reporter. [Stem Cells] Abstract

A Proapoptotic Effect of Valproic Acid on Progenitors of Embryonic Stem Cell-Derived Glutamatergic Neurons
Although the neuroprotective or neurodestructive effects of valproic acid (VPA) have been investigated in heterogeneous cell populations, in this study, researchers used homogeneous populations of neural progenitor cells and glutamatergic cortical pyramidal neurons, which were differentiated from embryonic stem cells. [Cell Death Dis] Abstract

The G Protein Coupled Receptor Apj Is Expressed in the Second Heart Field and Regulates Cerberus-Baf60C Axis in Embryonic Stem Cell Cardiomyogenesis
Researchers provided new in vivo evidence that the G protein coupled receptor Apj is expressed in the mesodermal cells of the second heart field, a population of cardiac progenitors that give rise to a major part of the definitive heart. By combining loss- and gain of function studies in mouse embryonic stem cells, they showed that Apj controls the balance between proliferation and cardiovascular differentiation, and regulates the Nodal/BMP antagonist Cerberus and the Baf60c/Smarcd3 subunit of the BAF chromatin remodeling complex. [Cardiovasc Res] Abstract

Proteome Analysis of Post-Transplantation Recovery Mechanisms of an EAE Model of Multiple Sclerosis Treated with Embryonic Stem Cell-Derived Neural Precursors
Investigators treated a score 3 experimental autoimmune encephalomyelitis (EAE) mouse model with an embryonic stem cell-derived neural precursor. Clinical analysis showed recovery of the EAE model of multiple sclerosis following transplantation. [J Proteomics] Abstract

Cooperation of Endothelial and Smooth Muscle Cells Derived from Human Induced Pluripotent Stem Cells Enhances Neovascularization in Dermal Wounds
Scientists assessed whether cotransplantation of endothelial cells and smooth muscle cells differentiated from human induced pluripotent stem cells could promote neovascularization and tissue repair in a murine dermal wound model. [Tissue Eng Part A] Abstract

Induced Pluripotent Stem Cell-Derived Cardiac Progenitors Differentiate to Cardiomyocytes and Form Biosynthetic Tissues
Researchers first described the derivation of mouse induced pluripotent stem cells, which once differentiated allow for the enrichment of Nkx2-5(+) cardiac progenitors, and the cardiomyocyte-specific expression of the red fluorescent protein. They showed that the cardiac progenitors are multipotent and capable of differentiating into endothelial cells, smooth muscle cells and cardiomyocytes. [PLoS One] Full Article

An iPSC Line from Human Pancreatic Ductal Adenocarcinoma Undergoes Early to Invasive Stages of Pancreatic Cancer Progression
Researchers hypothesized that if human pancreatic ductal adenocarcinoma (PDAC) cells were converted to pluripotency and then allowed to differentiate back into pancreatic tissue, they might undergo early stages of cancer. Although most induced pluripotent stem cell (iPSC) lines were not of the expected cancer genotype, one PDAC line, 10-22 cells, when injected into immunodeficient mice, generated pancreatic intraepithelial neoplasia precursors to PDAC that progressed to the invasive stage. [Cell Rep]
Abstract | Full Article | Graphical Abstract | Press Release

Stage-Specific Regulation of Reprogramming to Induced Pluripotent Stem Cells by Wnt Signaling and T Cell Factor Proteins
Investigators demonstrated that active Wnt signaling inhibits the early stage of reprogramming to induced pluripotent stem cells, whereas it is required and even stimulating during the late stage. [Cell Rep]
Abstract | Full Article | Graphical Abstract

Your Free Copy of the Nature Neuroscience Poster "Neural Stem Cells" Awaits

Opportunities and Challenges of Pluripotent Stem Cell Neurodegenerative Disease Models
Recent advances in pluripotent stem cell technology now grant access to substantial quantities of disease-pertinent neurons both with and without predisposing mutations. The authors review what they perceive to be several of the stumbling blocks in the use of stem cells for the study of neurological disease and offer strategies to overcome them. [Nat Neurosci] Abstract

Visit our reviews page to see a complete list of reviews in the ESC & iPSC research field.

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Takara Bio Grants Cellular Dynamics International a Commercial License to Use RetroNectin® for iPSC Production
Takara Bio Inc. announced that it has granted Cellular Dynamics International, Inc. (CDI) a non-exclusive, worldwide license to use Takara Bio’s RetroNectin® product. Based on the original methodology that CDI successfully first established to create induced pluripotent stem cells (iPSCs) with non-integrating episomal vectors, CDI has the ability to use RetroNectin® as one of its tools for the development and manufacture of its iCell® and MyCell® products. [Takara Bio Inc.] Press Release

University at Buffalo Study Seeks Molecular Cause of Parkinson’s Disease, Subtypes
Jian Feng, PhD, professor of physiology and biophysics, will use a $1 million state grant to explore molecular signatures of neurons in two types of Parkinson’s disease patients. He is generating midbrain, dopamine-releasing neurons from induced pluripotent stem cells derived from patients’ skin fibroblasts. [University at Buffalo, School of Medicine and Biomedical Sciences] Press Release

Grant Initiates New Stem Cell Research for Patients Suffering with Corneal Blindness
With a new vision for stem cell corneal transplantation, and a $1.25 million grant from the National Eye Institute, Cedars-Sinai co-investigators Alexander V. Ljubimov, PhD, FARVO, and Clive Svendsen, PhD, hope to treat previously untreatable patients suffering with corneal blindness. [Cedars-Sinai] Press Release


Japan Minister Defends New Growth Strategy Emphasizing Targeted Research
Japanese scientists are proving a bit skeptical about the administration’s new strategy for economic growth, which includes an emphasis on applied biomedical research. Even before it was officially released, several life science-related academic societies raised questions about plans for a Japanese version of the U.S. National Institutes of Health that would “strongly support the commercialization of innovative medical technologies.” [Minister for Economic Revitalization, Japan] Press Release

Japan to Relax Ban on Chimeric Embryo Experiments
A scientist pioneering research toward generating human organs in pigs has cleared one hurdle in gaining approval to continue his research in Japan. But he is still concerned that finalizing new research guidelines will take too long, so he is considering conducting the key experiments in the United States. [Japanese Government, Japan] Press Release


NEW Bioprocess Miniaturization: Development and Optimization
November 26, 2013
London, United Kingdom

NEW Recovery and Purification of Biosynthetic Products
November 27, 2013
London, United Kingdom

NEW New Procedures and Innovations for Analytical Development of Biopharmaceuticals
November 28, 2013
London, United Kingdom

NEW Applications for Synthetic Biology in Industrial Biotechnology
November 29, 2013
London, United Kingdom

Visit our events page to see a complete list of events in the ESC & iPSC community.

NEW Postdoctoral Position – Stem Cell Therapy and Epigenetics of Retina (University of Pittsburgh – Ophthalmology)

Postdoctoral Fellowship – Organic Synthesis and Medicinal Chemistry (Masaryk University)

Early Stage Researcher – Effect of Oxidative Damage on hES Cells Signaling Pathways (Avantea, Laboratory of Reproductive Technologies)

Director of Cell Processing Facility (S L Collins Associates, Inc.)

Postdoctoral Fellow – Cancer Immunology (Medical University of South Carolina)

Research Fellow – Cardiovascular Regenerative Applications (Department of Internal Medicine, Mayo Clinic)

Postdoctoral Research Fellowships – Embryonic Stem Cells, Epigenetics, and Gene Regulation (NIEHS, National Institutes of Health)

Postdoctoral Fellow – Stem Cell and Cancer Biology (Johns Hopkins University School of Medicine)

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