In Vivo Reprogramming of Astrocytes to Neuroblasts in the Adult Brain Scientists showed that the single transcription factor SOX2 is sufficient to reprogram resident astrocytes into proliferative neuroblasts in the adult mouse brain. These induced adult neuroblasts persist for months and can be generated even in aged brains. [Nat Cell Biol] Abstract The Non-Coding snRNA 7SK Controls Transcriptional Termination, Poising and Bidirectionality in Embryonic Stem Cells The authors identified the non-coding snRNA 7SK as a multifaceted regulator of transcription in embryonic stem cells. They found that 7SK represses a specific cohort of transcriptionally poised genes with bivalent or activating chromatin marks in these cells, suggesting a novel poising mechanism independent of Polycomb activity. [Genome Biol] Abstract | Full Article MBNL1 and RBFOX2 Cooperate to Establish a Splicing Program Involved in Pluripotent Stem Cell Differentiation The authors used high-throughput RT-PCR technology to take a snapshot of splicing changes in the full spectrum of high- and low-expressed genes during induction of fibroblasts, from several donors, into induced pluripotent stem cells and their subsequent redifferentiation. They uncovered a program of concerted alternative splicing changes involved in late mesoderm differentiation and controlled by key splicing regulators MBNL1 and RBFOX2. [Nat Commun] Abstract Efficient Derivation of Cortical Glutamatergic Neurons from Human Pluripotent Stem Cells: A Model System to Study Neurotoxicity in Alzheimer’s Disease Investigators implemented an efficient paradigm to convert human pluripotent stem cells into enriched populations of cortical glutamatergic neurons emerging from dorsal forebrain neural progenitors, aided by modulating Sonic hedgehog signaling. [Neurobiol Dis] Abstract Genomic and Proteomic Analyses of Prdm5 Reveal Interactions with Insulator Binding Proteins in Embryonic Stem Cells The authors found that Prdm5 is highly expressed in mouse embryonic stem cells and exploit this cellular system to characterize molecular functions of Prdm5. By combining proteomics and next generation sequencing technologies they identified Prdm5 interaction partners and genomic occupancy. [Mol Cell Biol] Abstract Niemann-Pick Type C1 Patient-Specific Induced Pluripotent Stem Cells Display Disease Specific Hallmarks Researchers reprogrammed human fibroblasts from a Niemann-Pick type C1 disease patient and a healthy control by retroviral transduction with Oct4, Klf4, Sox2 and c-Myc. The obtained human induced pluripotent stem cells were characterized by immunocytochemical analyses. [Orphanet J Rare Dis] Full Article Effects of Tet1 Knock-Down on Gene Expression and DNA Methylation in Porcine Induced Pluripotent Stem Cells To investigate the role of Tet1 in the pluripotency and differentiation of porcine induced pluripotent stem cells (piPSCs), piPSCs were obtained from porcine embryonic fibroblasts by overexpression of Oct4, Sox2, Klf4 and c-Myc. Four small interfering RNAs targeting to Tet1 were used to transiently knock-down the expression of Tet1 in piPSCs. [Reproduction] Abstract Requirement of B-Raf, C-Raf, and A-Raf for the Growth and Survival of Mouse Embryonic Stem Cells Researchers found that mouse embryonic stem (ES) cells express three forms of Raf kinases, A-Raf, B-Raf, and C-Raf. Knocking-down each single Raf member failed to affect the sustained extracellular signal-regulated kinases activity, neither did A-Raf and B-Raf double knockdown or B-Raf and C-Raf double knockdown change it in ES cells. [Exp Cell Res] Abstract |