Induction of Multipotential Hematopoietic Progenitors from Human Pluripotent Stem Cells via Respecification of Lineage-Restricted Precursors Researchers report a strategy to respecify lineage-restricted CD34+CD45+ myeloid precursors derived from human pluripotent stem cells into multilineage progenitors that can be expanded in vitro and engrafted in vivo. HOXA9, ERG, and RORA conferred self-renewal and multilineage potential in vitro and maintained primitive CD34+CD38– cells. [Cell Stem Cell] Abstract | Graphical Abstract | Press Release Global Transcriptional and Translational Repression in Human-Embryonic-Stem-Cell-Derived Rett Syndrome Neurons Scientists showed, using an isogenic human embryonic stem cell model of Rett syndrome, that MECP2 mutant neurons display key molecular and cellular features of this disorder. [Cell Stem Cell] Abstract | Graphical Abstract | Press Release Engineering Liver Tissue from Induced Pluripotent Stem Cells: A First Step in Generating New Organs for Transplantation? Researchers showed the generation of vascularized and functional human liver from human induced pluripotent stem cells by transplantation of liver buds created in vitro (iPSC-LBs). Specified hepatic cells (immature endodermal cells destined to track the hepatic cell fate) self-organized into three-dimensional iPSC-LBs by recapitulating organogenetic interactions between endothelial and mesenchymal cells. [Hepatology] Abstract Therapeutic Efficacy of Human Embryonic Stem Cell-Derived Endothelial Cells in Humanized Mouse Models Harboring a Human Immune System Scientists sought to examine the therapeutic potential of human embryonic stem cell-derived endothelial cells, human embryonic fibroblasts, and cord blood-derived endothelial progenitor cells in a human immune system environment. [Arterioscler Thromb Vasc Biol] Abstract Transcriptome Comparison of Human Neurons Generated Using Induced Pluripotent Stem Cells Derived from Dental Pulp and Skin Fibroblasts Dental pulp would be a good source for neurodevelopmental disorders in children because obtaining material is non-invasive. Scientists investigated its suitability for disease modeling by carrying out gene expression profiling, using RNA-seq, on differentiated neurons derived from induced pluripotent stem cell made from dental pulp extracted from deciduous teeth and fibroblasts. [PLoS One] Full Article A Novel Feeder-Free Culture System for Human Pluripotent Stem Cell Culture and Induced Pluripotent Stem Cell Derivation Investigators tested whether a commonly available human choriocarcinoma cell line, JAR, which produces high quantities of laminins, supports the growth of undifferentiated human pluripotent stem cells. [PLoS One] Full Article Ischemia, Immunosuppression, and SSEA-1-Negative Cells All Contribute to Tumors Resulting from Mouse Embryonic Stem Cell-Derived Neural Progenitor Transplantation Researchers investigated the tumor formation resulting from mouse embryonic stem cell-derived neural progenitor cells in a rat stroke model and in nude mice. Four weeks after transplantation, the grafted cells were found to be viable at the border of the necrosis and had differentiated into neurons. [J Neurosci Res] Abstract Analysis of Induced Pluripotent Stem Cells from a BRCA1 Mutant Family Researchers generated and characterized 24 induced pluripotent stem cell (iPSC) lines from fibroblasts of eight individuals from a BRCA1 5382insC mutant family. All BRCA1 5382insC heterozygous fibroblasts, iPSCs, and teratomas maintained equivalent expression of both wild-type and mutant BRCA1 transcripts. [Stem Cell Rep] Full Article Long-Term Self-Renewal of Human ES/iPS-Derived Hepatoblast-Like Cells on Human Laminin 111-Coated Dishes To purify and expand human pluripotent stem cell (PSC)-derived hepatoblast-like cells (HBCs), human PSC-derived HBCs were cultured on dishes coated with various types of human recombinant laminins. Human PSC-derived HBCs attached to human laminin-111 (LN111)-coated dish via integrin alpha 6 and beta 1 and were purified and expanded by culturing on the LN111-coated dish, but not by culturing on dishes coated with other laminin isoforms. [Stem Cell Rep] Full Article | Graphical Abstract |