Hhex and Cer1 Mediate the Sox17 Pathway for Cardiac Mesoderm Formation in Embryonic Stem Cells Previously, by RNA interference in AB2.2 mouse embryonic stem cells, the authors identified the endodermal transcription factor Sox17 as essential for Mesp1 induction in primitive mesoderm and subsequent cardiac muscle differentiation. In the present study, they used genome-wide profiling of Sox17-dependent genes in AB2.2 cells, RNA interference, chromatin immunoprecipitation, and luciferase reporter genes to dissect this pathway. [Stem Cells] Abstract GCNF-Dependent Activation of Cyclin D1 Expression via Repression of Mir302a during ES Cell Differentiation Scientists define the role of germ cell nuclear factor (GCNF) in the activation of cyclin D1 expression during embryonic stem (ES) cell differentiation as a model of early development. [Stem Cells] Abstract Micropattern Width Dependent Sarcomere Development in Human ESC-Derived Cardiomyocytes Human embryonic stem cell (ESC)-derived cardiomyocytes were seeded onto controlled two-dimensional micropatterned features, and an improvement in sarcomere formation and cell alignment was observed in specific feature geometries. [Biomaterials] Abstract | Press Release Phosphorylation of the Retinoic Acid Receptor RARγ2 Is Crucial for the Neuronal Differentiation of Mouse Embryonic Stem Cells Researchers investigated the in vivo relevance of RARs phosphorylation during retinoic acid-induced neuronal differentiation of mouse embryonic stem cells. [J Cell Sci] Abstract Graphene Oxide Promotes the Differentiation of Mouse Embryonic Stem Cells to Dopamine Neurons Scientists determined the effects of carbon nanotubes, graphene oxide and graphene on the dopamine neural differentiation of mouse embryonic stem cells. [Nanomedicine] Abstract Patient-Derived Skeletal Dysplasia iPSCs Display Abnormal Chondrogenic Marker Expression and Regulation by BMP2 and TGFβ1 To study early stages of aberrant cartilage formation in vitro, researchers generated the first induced pluripotent stem cells (iPSCs) from fibroblasts of a skeletal dysplasia patient with a lethal form of metatropic dysplasia, caused by a dominant mutation (in the calcium channel gene TRPV4. [Stem Cell Dev] Abstract Soluble Factors Secreted by Differentiating Embryonic Stem Cells Stimulate Exogenous Cell Proliferation and Migration Embryonic stem cells were differentiated as embryoid bodies (EBs) in vitro and media conditioned by EBs was collected at different intervals of time. Gene and protein expression analysis of several different growth factors secreted by EBs were examined by PCR and ELISA analysis, respectively, as a function of time. [Stem Cell Res Ther] Abstract | Full Article Evidences for the Involvement of Cell Surface Glycans in Stem Cell Pluripotency and Differentiation The author characterized the surface glycans of induced pluripotent stem cells generated from menstrual blood-derived mesenchymal cells (iPS-MBMC). They demonstrated that, upon spontaneous differentiation, iPS-MBMC present high amounts of terminal β-galactopyranoside residues, pointing to an important role of terminal-linked sialic acids in pluripotency maintenance. [Glycobiology] Abstract An Induced Pluripotent Stem Cell Model of Hypoplastic Left Heart Syndrome (HLHS) Reveals Multiple Expression and Functional Differences in HLHS-Derived Cardiac Myocytes Using recent advances in the field of induced pluripotent stem cells (iPSCs), investigators were able to generate an iPSC model of HLHS malformation and characterize the properties of cardiac myocytes differentiated from these and control-iPSC lines. [Stem Cells Transl Med] Abstract |