Function-Based Identification of Mammalian Enhancers Using Site-Specific Integration By functionally interrogating >500 kilobases of mouse and human sequence in mouse embryonic stem cells for enhancer activity investigators identified enhancers at pluripotency loci including NANOG. [Nat Methods] Abstract | Press Release FIREWACh: High-Throughput Functional Detection of Transcriptional Regulatory Modules in Mammalian Cells The authors report a high-throughput functional assay for directly identifying active promoter and enhancer elements called FIREWACh (Functional Identification of Regulatory Elements Within Accessible Chromatin), which they used to simultaneously assess over 80,000 DNA fragments derived from nucleosome-free regions within the chromatin of embryonic stem cells and identify 6,364 active regulatory elements. [Nat Methods] Abstract Transcripts Involved in Calcium Signaling and Telencephalic Neuronal Fate Are Altered in Induced Pluripotent Stem Cells from Bipolar Disorder Patients Investigators examined changes in gene expression as induced pluripotent stem cell (iPSC) derived from well-characterized patients differentiate into neurons; there was little difference in the transcriptome of iPSC, but bipolar disorder neurons were significantly different than controls in their transcriptional profile. [Transl Psychiatry] Full Article | Press Release Retinoic Acid Suppresses the Canonical Wnt Signaling Pathway in Embryonic Stem Cells and Activates the Noncanonical Wnt Signaling Pathway The activation of the canonical Wnt signaling pathway, which requires the nuclear accumulation of β-catenin and its interaction with Transcription factor 7/Lef at Wnt response elements, is involved in embryonic stem cells stemness maintenance. The noncanonical Wnt signaling pathway, through actions of Transcription factor 7-like 1, can antagonize the canonical pathway. Scientists showed that retinoic acid activates the noncanonical Wnt signaling pathway, while concomitantly inhibiting the canonical pathway. [Stem Cells] Abstract Coactivators p300 and CBP Maintain the Identity of Mouse Embryonic Stem Cells by Mediating Long-Range Chromatin Structure The authors examined the function of two highly homologous coactivators, p300 and CREB-binding protein (CBP), in embryonic stem (ES) cells. They found that these two coactivators play redundant roles in maintaining the undifferentiated state of ES cells. [Stem Cells] Abstract Induced Pluripotent Stem Cell Derived Forebrain Neurons from FXS Individuals Show Defects in Initial Neurite Outgrowth Investigators created human induced pluripotent stem cell lines from fibroblasts obtained from individuals with Fragile X syndrome (FXS) to enable in vitro modeling of the human disease. [Stem Cells Dev] Abstract Osteogenesis from Human Induced Pluripotent Stem Cells: An In Vitro and In Vivo Comparison with Mesenchymal Stem Cells Osteo-induced human induced pluripotent stem cells were implanted on the critical-size calvarial defects and long bone segmental defects in rats. The healing of defects was evaluated after 8 weeks and 12 weeks of implantation respectively. [Stem Cells Dev] Abstract Induction of Human Embryonic and Induced Pluripotent Stem Cells into Urothelium Investigators developed an efficient in vitro protocol for the induction of human embryonic stem cells into urothelium through an intermediary definitive endoderm step and free of matrices and cell contact. [Stem Cell Transl Med] Abstract | Press Release Human Finger-Prick Induced Pluripotent Stem Cells Facilitate the Development of Stem Cell Banking Researchers describe the efficient derivation of transgene-free human induced pluripotent stem cells (hiPSCs) from human finger-prick blood. Finger-prick sample collection can be performed on a “do-it-yourself” basis by donors and sent to the hiPSC facility for reprogramming. [Stem Cells Transl Med] Abstract | Press Release |