High-Resolution Mapping of Chromatin Packaging in Mouse Embryonic Stem Cells and Sperm Investigators extended a recently developed technique, based on deep paired-end sequencing of lightly digested chromatin, to assess footprints of nucleosomes and other DNA-binding proteins genome-wide in murine embryonic stem cells and sperm. [Dev Cell] Abstract Modeling Fanconi Anemia Pathogenesis and Therapeutics Using Integration-Free Patient-Derived iPSCs Scientists derived integration free-induced pluripotent stem cells (iPSCs) from an Fanconi anemia (FA) patient without genetic complementation and report in situ gene correction in FA-iPSCs as well as the generation of isogenic FANCA-deficient human embryonic stem cell lines. [Nat Commun] Abstract Induction of Primordial Germ Cell-Like Cells from Mouse Embryonic Stem Cells by ERK Signal Inhibition Coculture of mouse embryonic stem cells (ESCs) with OP9 feeder cells induces mesodermal differentiation in vitro. Using this mesodermal differentiation system, researchers demonstrate that primordial germ cell-like cells were efficiently induced from mouse ESCs by ERK signaling inhibition. [Stem Cells] Abstract Cell Reprogramming into the Pluripotent State Using Graphene Based Substrates The authors report that graphene promotes the reprogramming of mouse somatic fibroblasts into induced pluripotent stem cells. They found that the graphene substrate significantly improved cellular reprogramming efficiency by inducing mesenchymal-to-epithelial-transition which is known to affect H3K4me3 levels. [Biomaterials] Abstract Footprint- and Xeno-Free Human iPSCs Derived from Urine Cells Using Extracellular Matrix-Based Culture Investigators established a practical, simple, and convenient method to generate integration- and xeno-free induced pluripotent stem cells (iPSCs) from urine cells which can be obtained in a non-invasive manner. [Biomaterials] Abstract Human Embryonic Stem Cells and Macroporous Calcium Phosphate Construct for Bone Regeneration in Cranial Defects in Rats Scientists investigated human embryonic stem cells for bone regeneration in vivo in critical-sized cranial defects in rats and determined the effects of cell seeding and platelets in macroporous calcium phosphate cement on new bone and blood vessel formation. [Acta Biomater] Abstract Rad51 Regulates Cell Cycle Progression by Preserving G2/M Transition in Mouse Embryonic Stem Cells Rad51 and homologous recombination accessory factors may facilitate replication fork progression by preventing replication fork collapse and repair double-strand breaks that spontaneously arise during the normal cell cycle. Researchers demonstrate a novel role for Rad51 during the cell cycle in mouse embryonic stem cells. [Stem Cells Dev] Abstract Notch-1 Mediated Cardiac Protection following Embryonic and Induced Pluripotent Stem Cell Transplantation in Doxorubicin-Induced Heart Failure Researchers reveal apoptosis was significantly inhibited in embryonic stem and induced pluripotent stem cell transplanted hearts compared with respective controls. [PLoS One] Full Article Somatostatin Receptor Type 2 Contributes to the Self-Renewal of Murine Embryonic Stem Cells The roles of G-protein coupled receptors (GPCRs) in stem cell biology remain unclear. Investigators aimed to identify GPCRs that might contribute to the self-renewal of mouse embryonic stem cells. [Acta Pharmacol Sin] Abstract |