Genetic and Molecular Identification of Three Human TPP1 Functions in Telomerase Action: Recruitment, Activation, and Homeostasis Set Point Regulation Using genome engineering of human embryonic stem cells, which have physiological telomere length homeostasis, we establish that the TPP1 TEL patch is genetically essential for telomere elongation and thus long-term cell viability. [Genes Dev] Abstract Nanovesicles Engineered from ES Cells for Enhanced Cell Proliferation Research on exosomes is limited by their rarity and heterogeneity even after lengthy isolation processes. In particular, these limitations are challenging in therapeutic applications. To meet these demands, cell-derived nanovesicles that mimic exosomes were generated by extruding living embryonic stem (ES) cells through micro-filters. [Biomaterials] Abstract The Organophosphorus Pesticide Chlorpyrifos and Its Metabolites Alter the Expression of Biomarker Genes of Differentiation in D3 Mouse Embryonic Stem Cells in a Comparable Way to Other Model Neurodevelopmental Toxicants The authors previously reported alterations in the pattern of expression of biomarker genes of differentiation in D3 mouse embryonic stem cells caused by chlorpyrifos and its metabolites chlorpyrifos-oxon and 3,5,6-trichloro-2-pyridinol. Now, they reanalyzed these data comparing the effects on these genes with those caused in the same genes by retinoic acid, valproic acid and penicillin-G (model compounds considered as strong, weak and non-neurodevelopmental toxicants, respectively). [Chem Res Toxicol] Abstract A Site-Specific Integrated Col2.3GFP Reporter Identifies Osteoblasts Within Mineralized Tissue Formed In Vivo by Human Embryonic Stem Cells Investigators used zinc finger nuclease technology to deliver a construct containing the Col2.3 promoter driving GFPemerald to the AAVS1 site in human embryonic stem cells with the goal of marking the cells that have become differentiated osteoblasts. [Stem Cells Transl Med] Abstract Cost-Effective Master Cell Bank Validation of Multiple Clinical-Grade Human Pluripotent Stem Cell Lines from a Single Donor In order to realize the full potential of stem cell therapy, validation criteria, methodology, and, most importantly, strategy, should address the shortfalls and efficiency of current approaches. Investigators addressed the protocols for testing cell lines for human viral pathogens and propose a novel strategy that would significantly reduce costs. [Stem Cells Transl Med] Abstract Exploring the Mechanisms of Differentiation, Dedifferentiation, Reprogramming and Transdifferentiation Scientists show that under three driving forces, the direct transdifferentiation process proceeds from a differentiated cell valley to another differentiated cell valley through either a distinct stable intermediate state or a certain series of unstable indeterminate states. [PLoS One] Full Article CAR Expression in Human Embryos and hESC Illustrates Its Role in Pluripotency and Tight Junctions Scientists investigated the expression of coxsackie virus and adenovirus receptor (CAR) in human preimplantation embryos and embryonic stem cells (hESC) to identify its role in early embryogenesis and differentiation. [Reproduction] Abstract Generation of Intermediate Porcine iPS Cells under Culture Condition Favorable for Mesenchymal-to-Epithelial Transition Researchers investigated whether intermediate pluripotent states could be available by manipulating the culture condition during the process of generating pig induced pluripotent stem cells. [Stem Cell Rev] Abstract |