An In Vivo Model of Human Small Intestine Using Pluripotent Stem Cells Researchers have successfully transplanted “organoids” of functioning human intestinal tissue grown from pluripotent stem cells in a lab dish into mice – creating an unprecedented model for studying diseases of the intestine. [Nat Med] Abstract | Press Release A Nontranscriptional Role for Oct4 in the Regulation of Mitotic Entry Researchers inquired how ES cells avoid the potentially deleterious consequences of premature mitotic entry. They found that the pluripotency transcription factor octamer-binding transcription factor 4 (Oct4) plays an unappreciated role in the ES cell cycle by forming a complex with cyclin-dependent kinase (Cdk)1 and inhibiting Cdk1 activation. [Proc Natl Acad Sci USA] Abstract A Thyroid Hormone Receptor/KLF9 Axis in Human Hepatocytes and Pluripotent Stem Cells The authors show that triiodothyronine also works through thyroid hormone receptors (TR) to induce Kruppel-like factor 9 (KLF9) in HepG2 liver cells, mouse liver and mouse and human primary hepatocytes and sought to understand TR/KLF9 network function in the hepatocyte lineage and stem cells. [Stem Cells] Abstract The Combination of Tet1 with Oct4 Generates High-Quality Mouse Induced Pluripotent Stem Cells (iPSCs) Scientists provide evidence that Tet1 can replace multiple transcription factors during somatic cell reprogramming and can generate high-quality mouse induced pluripotent stem cells with Oct4. [Stem Cells] Abstract Erythroid Differentiation of Human Induced Pluripotent Stem Cells Is Independent of Donor Cell Type of Origin To investigate the influence of an epigenetic memory on the ex vivo expansion of induced pluripotent stem cells into erythroid cells, scientists compared induced pluripotent stem cells from human neural stem cells and human cord blood-derived CD34+ hematopoietic stem cells and evaluated their potential for differentiation into hematopoietic progenitor and mature red blood cells. [Haematologica] Abstract | Full Article Proteomics of Huntington’s Disease Affected Human Embryonic Stem Cells Reveals an Evolving Pathology Involving Mitochondrial Dysfunction and Metabolic Disturbances Scientists conducted a proteomics study combined with immunocytochemical analysis of undifferentiated Huntington’s disease (HD)-affected and unaffected human embryonic stem cells. [J Proteome Res] Abstract Histologic and Spectroscopic Study of Pluripotent Stem Cells after Implanting in Ocular Traumatic Injuries in a Murine Model Mouse embryonic stem cells were seeded, and the obtained cells were implanted in a murine model of scleral and retinal damage at the first, second and fourth week post-trauma. At week 12 post-trauma, the eyes were enucleated for histopathologic study and spectroscopic analysis by Fourier transform infrared spectroscopy in the attenuated total reflection configuration. [Stem Cell Res Ther] Abstract | Full Article NRG-1b Induces Mature Ventricular Cardiac Differentiation from Induced Pluripotent Stem Cells Contributing to Cardiac Tissue Repair Scientists examined whether neuregulin-1b (NRG-1b) treatment could enhance in vitro generation of mature “working-type” stem cell-derived cardiomyocytes (CMs) from induced pluripotent stem cells and assessed the regenerative effects of these CMs on cardiac tissue following acute myocardial infarction. [Stem Cells Dev] Abstract Scalable Generation of Universal Platelets from Human Induced Pluripotent Stem Cells Researchers describe a method to generate megakaryocytes (MKs) and functional platelets from human induced pluripotent stem cells in a scalable manner under serum/feeder-free conditions. The method also permits the cryopreservation of MK progenitors, enabling a rapid “surge” capacity when large numbers of platelets are needed. [Stem Cell Rep] Full Article | Press Release |