The Role of Maternal-Specific H3K9me3 Modification in Establishing Imprinted X-Chromosome Inactivation and Embryogenesis in Mice Scientists show, using a highly reproducible chromatin immunoprecipitation method that facilitates chromatin analysis of preimplantation embryos, that H3K9me3 is enriched at the Xist promoter region, preventing maternal Xist activation by RNF12. The high levels of H3K9me3 at the Xist promoter region are lost in embryonic stem (ES) cells, and ES-cloned embryos show RNF12-dependent Xist expression. [Nat Commun] Full Article Foxd3 Suppresses NFAT-Mediated Differentiation to Maintain Self-Renewal of Embryonic Stem Cells Researchers report that Foxd3 suppresses differentiation induced by calcineurin-NFAT signaling to maintain the ESC identity. Mechanistically, Foxd3 interacts with NFAT proteins and recruits co-repressor Tle4, a member of the Tle repressor family highly expressed in undifferentiated ESCs, to suppress NFATc3’s transcriptional activities. [EMBO Rep] Abstract Functional Compensation between Myc and PI3K Signaling Supports Self-Renewal of Embryonic Stem Cells Scientists demonstrate that c-Myc and PI3K function cooperatively but independently to support embryonic stem cell (ESC) self-renewal when murine ESCs are cultured under conventional culture condition. [Stem Cells] Abstract CDK1 Plays an Important Role in the Maintenance of Pluripotency and Genomic Stability in Human Pluripotent Stem Cells To investigate the impacts of cyclin-dependent kinase 1 (CDK1) downregulation, researchers performed RNA interference studies which in addition to expected mitotic deficiencies revealed a large range of additional phenotypes related to maintenance of pluripotency, ability to repair double strand breaks and commitment to apoptosis. [Cell Death Dis] Full Article The Secretome of Induced Pluripotent Stem Cells Reduces Lung Fibrosis in Part by Hepatocyte Growth Factor Investigators evaluated the potential of induced pluripotent stem cells conditioned media to regenerate and repair the alveolar epithelium in vitro and improve bleomycin induced lung injury in vivo. [Stem Cell Res Ther] Abstract | Full Article Nanog Requires BRD4 to Maintain Murine Embryonic Stem Cell Pluripotency and Is Suppressed by Bromodomain Inhibitor JQ1 Together with Lefty1 Investigators demonstrate that the pan-BET (bromodomain and extra-terminal domain) bromodomain inhibitor JQ1 induces rapid spontaneous differentiation of murine embryonic stem cells by inducing marked transcriptional down-regulation of Nanog as well as the stemness markers Lefty1 and Lefty2 but not Myc, often used as a marker of BET inhibitor activity in cancer. [Stem Cells Dev] Abstract The Effect of 3D Nanofibrous Scaffolds on the Chondrogenesis of Induced Pluripotent Stem Cells and Their Application in Restoration of Cartilage Defects Researchers investigated the effect of 3D nanofibrous scaffolds on the chondrogenesis of induced pluripotent stem cells (iPSCs) and articular cartilage defect restoration. The chondrogenesis of the iPSCs in vitro and the restoration of the cartilage defect was investigated using scanning electron microscopy, the Cell Counting Kit-8, histological observation, RT-qPCR, and western blot analysis. [PLoS One] Full Article Reprogramming Suppresses Premature Senescence Phenotypes of Werner Syndrome Cells and Maintains Chromosomal Stability over Long-Term Culture Scientists describe the effects of long-term culture on Werner syndrome induced pluripotent stem cells, which acquired and maintained infinite proliferative potential for self-renewal over two years. [PLoS One] Full Article | Press Release Human Embryonic Stem Cells Carrying Unbalanced Translocations Demonstrate Impaired Differentiation into Trophoblasts: An In-Vitro Model of Human Implantation Failure Scientists derived the first human embryonic stem cell (hESC) line carrying unbalanced t(11;22), which showed the typical morphological and molecular characteristics of a hESC line. Control hESCs differentiated into trophoblasts secreted increasing levels of beta-hCG and concomitantly expressed the trophoblast genes, CDX2, TP63, KRT7, ERVW1, CGA, GCM1, KLF4 and PPARG. [Mol Hum Reprod] Abstract Effects of Mouse Utricle Stromal Tissues on Hair Cell Induction from Induced Pluripotent Stem Cells Clarification of the molecular mechanisms underlying inner ear disorders is also impeded by the anatomical limitation of experimental access to the human inner ear. Therefore, the generation of hair cells, possibly from induced pluripotent stem (iPS) cells, is important for regenerative therapy and studies of inner ear diseases. Researchers generated hair cells from mouse iPS cells using an established stepwise induction protocol. [BMC Neurosci] Full Article |