Extracellular Matrix News 10.01 January 17, 2019 | |
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TOP STORYHIF-1α Metabolically Controls Collagen Synthesis and Modification in Chondrocytes Researchers showed that prolonged HIF-1α signaling in chondrocytes led to skeletal dysplasia by interfering with cellular bioenergetics and biosynthesis. Decreased glucose oxidation resulted in an energy deficit, which limited proliferation, activated the unfolded protein response and reduced collagen synthesis. However, enhanced glutamine flux increased α-ketoglutarate levels, which in turn increased proline and lysine hydroxylation on collagen. [Nature] Abstract | |
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PUBLICATIONS(Ranked by impact factor of the journal)Activated PMN Exosomes: Pathogenic Entities Causing Matrix Destruction and Disease in the Lung Scientists describe a novel pathogenic entity, the activated PMN (polymorphonuclear leukocyte, i.e., neutrophil)-derived exosome. These exosomes bound and degraded the ECM via the integrin Mac-1 and neutrophil elastase, respectively, causing the hallmarks of chronic obstructive pulmonary disease. [Cell] Abstract | Press Release | Graphical Abstract Researchers produced cornea-shaped, curved stromal tissue equivalents via the controlled, cell-driven curving of collagen-based hydrogels. The self-curved constructs were then characterized in terms of cell and collagen fibril reorganization, gel stiffness, cell phenotype, and the ability to sustain the growth of a corneal epithelium in vitro. [Adv Funct Mater] Abstract | Press Release Primary patient-derived CAFs were cultured in 3D melt electrowritten scaffolds where they deposited extensive ECM and promoted significant changes in prostate epithelial morphology, when compared to matched non-malignant prostatic fibroblasts. [Biomaterials] Abstract Through a combination of methylation, hydroxymethylation, and transcript profiling, the genes encoding the activin A subunit Inhbaa and Sox9b were implicated as demethylation targets of ten-eleven translocation (TET) enzymes 2/3 and critical for organization of atrial-ventricular canal-localized ECM, facilitating migration of epicardial progenitors onto the developing heart tube. [Cell Rep] Full Article | Graphical Abstract YAP1/Twist Promotes Fibroblast Activation and Lung Fibrosis That Conferred by miR-15a Loss in IPF Investigators found that overexpression of YAP1, a key effector in the Hippo pathway, promoted cell proliferation, migration, and collagen production in lung fibroblasts. Knockdown of YAP1 inhibited ECM deposition, which ultimately ameliorated lung fibrosis in vitro and in vivo. [Cell Death Differ] Abstract FSTL1 directly increased the expression of matrix metalloproteinase 1 (MMP-1), MMP-13, inducible nitric oxide synthase, cyclooxygenase-2, interleukin (IL)-1β, tumor necrosis factor-α and IL-6 at both gene and protein level in a dose-dependent manner. Activation of nuclear factor kappa B (NF-κB) and phosphorylation of p65 were also promoted by FSTL1 stimulation. [J Cell Mol Med] Full Article Identification of Chondrocyte Genes and Signaling Pathways in Response to Acute Joint Inflammation Catabolic genes related to ECM degeneration were promoted by IL-1β, consistent with the authors’ observations of matrix protein loss and mechanical property decrease during 24-day in vitro culture of cartilage explants. [Sci Rep] Full Article 3D Culture of Salivary Gland Stem Cell in Orthotropic Decellularized Extracellular Matrix Hydrogels Scientists developed the first rat salivary gland (SG) tissue-derived decellularized ECM hydrogel (DSGM-hydrogel) as a functional orthotropic bio-scaffold for future efficient SG stem cell therapy. DSGM-hydrogels were characterized by rheological or biochemical analyses and rat SG stem/progenitor cells were then subjected to three-dimensional culture in the DSGM-hydrogels. [Tissue Eng Part A] Abstract Hydrogen peroxide and buthionine sulfoximine resulted in a notable loss of the endothelial glycocalyx; they also increased the expression and the proteolytic activity of matrix metalloproteinase 2 (MMP2) and MMP9 and subsequently increased the shedding of syndecan-1 and superoxide dismutase from endothelial cell surface. [Am J Physiol Heart Circ Physiol] Abstract ENA-78/CXCL5 and ICAM-1 were decreased in tumor necrosis factor (TNF)-α-stimulated A disintegrin and metalloprotease 15 (ADAM15) siRNA-transfected human umbilical vein endothelial cell (HUVEC)-conditioned medium compared with TNF-α-stimulated control siRNA-transfected HUVEC-conditioned medium. [Cells] Full Article Subscribe to one of our other 19 science newsletters such as Endothelial Cell News & Dermal Cell News. | |
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REVIEWSModeling Cancer in Microfluidic Human Organs-on-Chips The authors outline how recent developments in microfluidic cell culture technology have led to the generation of human organs-on-chips that are now being used to model cancer cell behavior within human-relevant tissue and organ microenvironments in vitro. [Nat Rev Cancer] Abstract Visit our reviews page to see a complete list of reviews in the extracellular matrix research field. | |
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SCIENCE NEWSAnika Announces Plans to Showcase HYALOFAST Anika Therapeutics, Inc. announced plans to showcase HYALOFAST, a biodegradable, hyaluronic acid-based cartilage repair scaffold. [Press release from Anika Therapeutics, Inc. discussing research presented at the 2019 International Cartilage Repair Society (ICRS) Summit, San Diego] Press Release | |
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INDUSTRY NEWSGenerex Biotechnology Corporation announced that Olaregen Therapeutix Inc., a subsidiary of Generex Biotechnology will launch three doses of FDA 510K-Cleared Excellagen, the company’s wound conforming gel matrix that is designated as a Cellular & Tissue Based Product or CTP for wound healing. [Generex Biotechnology Corp.] Press Release LIU’s Dr. Richard Nader Is Awarded Fulbright Scholarship to India Dr. Richard Nader, Chief Research and International Officer at Long Island University (LIU), has been selected for a 2019-2020 Fulbright-Nehru U.S. International Education Administrators award to India. This particular Fulbright program is a specially designed, short-term intensive program to connect U.S. universities to opportunities in India that match the interests and priority areas of LIU. [Long Island University] Press Release | |
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POLICY NEWSCrash-Out Brexit Looms Larger for Scientists after Deal Rejected A historic defeat for U.K. Prime Minister Theresa May has raised the odds that the United Kingdom will crash out of the European Union in March, a prospect that scientists dread for its potential for disruption to research collaborations and the economy. [ScienceInsider] Editorial German Institutions and Wiley Reach Open-Access Publishing Deal Ater nearly three years of contract negotiations, a consortium of nearly 700 German libraries, research institutes, and universities called Project DEAL has forged an agreement with the scholarly publisher Wiley that moves the publishing industry towards more open access. [The Scientist] Editorial Mass Student Protests Bring Down Albania’s Science Minister Albania’s education and science minister has been replaced in a government reshuffle amid weeks of mass student protests that brought the country’s public universities to a halt. For years, students have been complaining about growing privatization of education, which, they say, has hiked fees without adding benefits. [Nature News] Editorial
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JOB OPPORTUNITIESNEW Tenure Track Associate Professor – Molecular Cell Biology (Aarhus University) Scientist – Matrix Biology (STEMCELL Technologies Inc.) Tenure Track Assistant Professor – Regenerative Medicine (University of British Columbia) Associate Senior Lecturer – Molecular Medicine/Regenerative Medicine (Lund University) Postdoctoral Associate – Planar Cell Polarity (Stony Brook University) Tenure Track Faculty Position – Department of Pharmacology & Physiology (University of Rochester) Postdoctoral Fellowship – Tissue Fibrosis Resolution (UCSF Cardiovascular Research Institute) Assistant Professor – Sarcoma & Skeletal-Related Malignancies (UC Davis School of Medicine) Postdoctoral Fellow – Extracellular Matrix (UCSF School of Medicine) Recruit Top Talent: Reach potential candidates by posting your organization’s career opportunities on the Connexon Creative Job Board at no cost.
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