A Fibronectin-Independent Mechanism of Collagen Fibrillogenesis in Adult Liver Remodeling Researchers generated a conditional, fibronectin-deficient mouse model of liver injury and explored whether fibronectin would be a suitable target for preventing extensive collagen deposits and scar formation that could lead to liver fibrosis. [Gastroenterology] Nanolithographic Control of the Spatial Organization of Cellular Adhesion Receptors at the Single-Molecule Level Researchers have developed a technique which combines molecular-scale nanolithography with site-selective biochemistry to create biomimetic arrays of individual protein binding sites. The binding sites can be arranged in heterogeneous patterns of virtually any possible geometry with a nearly unlimited number of degrees of freedom. Researchers used these arrays to explore how the geometric organization of the extracellular matrix binding ligand RGD (Arg-Gly-Asp) affects cell adhesion and spreading. [Nano Lett] The Role of Extracellular Matrix Composition in Structure and Function of Bioengineered Skeletal Muscle Researchers hypothesized that engineered muscle’s force production and transmission would be profoundly influenced by cell-matrix interactions. To test this hypothesis, they systematically varied the matrix protein type (collagen I/fibrin/Matrigel) and concentration in engineered, hydrogel-based neonatal rat skeletal muscle bundles and assessed the resulting tissue structure, generation of contractile force, and intracellular Ca2+ handling. [Biomaterials] The Performance of Laminin-Containing Cryogel Scaffolds in Neural Tissue Regeneration Researchers describe neurogenic properties of bioactive scaffolds generated by cryogelation of dextran or gelatin linked to laminin — the main component of brain extracellular matrix. [Biomaterials] Layer-by-Layer Films Made from Extracellular Matrix Macromolecules on Silicone Substrates Researchers assessed the build-up of type I collagen (Col1)/chondroitin sulfate or Col1/Heparin on polydimethylsiloxane substrates. [Biomacromolecules] A New Insight in the Dissociating Effect of Strontium on Bone Resorption and Formation Researchers investigated the capacities of their strontium-substituted sol-gel calcium phosphate to modify genes expression and proteins involved in extracellular matrix synthesis by primary bone cells. [Acta Biomater] Granzyme B Contributes to Extracellular Matrix Remodeling and Skin Aging in Apolipoprotein E Knockout Mice Results provide novel insights into the effects of a high fat diet and apolipoprotein E deficiency on skin aging and pathology and suggest a role for granzyme B in age-related skin thinning and frailty. [Exp Gerontol] The Role of the Cytoskeleton in Cellular Force Generation in 2D and 3D Environments Data suggest that despite differences in cytoskeletal organization in 2D and 3D, actin, microtubules and myosin contribute to contractility and matrix reorganization similarly in both microenvironments. [Phys Biol] Influence of Glial-Derived Matrix Molecules, Especially Chondroitin Sulfates, on Neurite Growth and Survival of Cultured Mouse Embryonic Motoneurons Results indicate that molecules of the extracellular matrix, especially chondroitin sulfates, play an important role as axon growth-promoting cues. [J Neurosci Res] |