Ideal Scaffolds that Represent Native Extracellular Matrix (ECM) Properties of Musculofascial Tissues Have Great Importance in Musculofascial Tissue Investigators developed a decellularization protocol for processing pig composite musculofascial tissues. Decellularized muscle and decellularized fascia, which are two important components of decellularized musculofascial ECM, were comprehensively characterized. [Biomaterials] Abstract Leptin-Mediated Regulation of MT1-MMP Localization Is Kinesin Family Member 1B-Dependent and Enhances Gastric Cancer Cell Invasion The authors characterized the influence of leptin on the generation and surface localization of membrane type 1-matrix metalloproteinase (MT1-MMP) in gastric cancer (GC) and elucidated its molecular mechanisms. Results revealed that leptin promoted GC cell invasion in vitro by upregulating MT1-MMP expression. [Carcinogenesis] Abstract Hyaluronic Acid Click Hydrogels Emulate the Extracellular Matrix This study demonstrates the versatility of hyaluronic acid based hydrogels with independent control over hydrogel properties such as mechanics, architecture, and spatial distribution of biological factors. Hydrogels were prepared by reacting furan-modified hyaluronic acid with bis-maleimide-poly(ethylene glycol) in a Diels-Alder click reaction. [Langmuir] Abstract Extracellular Matrix Protein Lumican Promotes Clearance and Resolution of Pseudomonas aeruginosa Keratitis in a Mouse Model Lumican is a major component of the cornea, yet its role in counteracting live bacteria in the cornea remains poorly understood. Scientists investigated Pseudomonas aeruginosa infections of the cornea in lumican deficient mice. [PLoS One] Full Article The Impact of Compact Layer in Biphasic Scaffold on Osteochondral Tissue Engineering A novel biphasic scaffold composed of a bony phase, chondral phase and compact layer was developed. The compact layer-free biphasic scaffold taken as control group was also fabricated. Researchers evaluated the impact of the compact layer in the biphasic scaffold. [PLoS One] Full Article Comparison of In Vivo Adipogenic Capabilities of Two Different Extracellular Matrix Microparticle Scaffolds Adipose tissue extract microparticle scaffolds from human adipose tissue and small intestine submucosa microparticle scaffolds from porcine jejunum were prepared. Their effects on the adipogenic capabilities of human adipose-derived stem cells were compared in vivo. [Plast Reconstr Surg] Abstract Stage Specific Effect of Leptin on the Expressions of Estrogen Receptor and Extracellular Matrix in a Model of Chondrocyte Differentiation Researchers investigated the effect of leptin on the expression of estrogen receptors and extracellular matrix in ATDC5 cells, an in vitro model of endochondral ossification. [Cytokine] Abstract Collagen/Beta1 Integrin Interaction Is Required for Embryoid Body Formation during Cardiogenesis from Murine Induced Pluripotent Stem Cells The role of extracellular matrix/integrin interaction in the formation of embryoid bodies (EBs) during cardiogenesis from murine induced pluripotent stem cells (miPSCs) remains unclear. Here, collagen type I and beta1 integrin were expressed and upregulated synergistically during the formation of miPSC-derived EBs, with a peak expression at day three of differentiation. [BMC Cell Biol] Abstract | Full Article In Vitro Evaluation of the Interactions between Human Corneal Endothelial Cells and Extracellular Matrix Proteins To evaluate the interaction of human corneal endothelial cells (CECs) with extracellular matrix (ECM) proteins, cells were seeded on ECM-coated tissue culture dishes, including collagen type I, collagen type IV, fibronectin (FN), FN coating mix and laminin. Cell adhesion and proliferation of human CECs on each substratum and expression of CEC markers were studied. [Biomed Mater] Full Article |