Efficiencies of Fragmentation of Glycosaminoglycan Chloramides of the Extracellular Matrix by Oxidizing and Reducing Radicals: Potential Site-Specific Targets in Inflammation? The N-Cl group in the chloramides is a potential selective target for both reducing and oxidizing radicals, leading possibly to more efficient and damaging fragmentation of these biopolymers relative to the parent glycosaminoglycans. To investigate the effect of the N-Cl group, ionizing radiation has been used to produce quantifiable concentrations of the reducing radicals, the hydrated electron and the superoxide radical and also of the oxidizing radicals, hydroxyl, carbonate and nitrogen dioxide, all of which have been reacted with hyaluronan and heparin and their chloramides in the current study. [Free Radic Biol Med] Abstract Breast Cancer Normalization Induced by Embryonic Mesenchyme Is Mediated by Extracellular Matrix Biglycan Using a synthetic reconstitution system, scientists showed that co-culture of breast cancer cells with embryonic mesenchyme from early stage mammary glands decreases tumor cell proliferation while stimulating acinus differentiation, whereas cancer-associated fibroblasts fail to produce these normalizing effects. When insoluble extracellular matrices were isolated from cultured early stage embryonic mammary mesenchyme cells or E10 tooth mesenchyme and recombined with mammary tumor cells, they were found to be sufficient to induce breast cancer normalization including enhanced expression of estrogen receptor-α. [Integr Biol] Abstract Podoplanin-Mediated Cell Adhesion through Extracellular Matrix in Oral Squamous Cell Carcinoma Researchers hypothesized that podoplanin (PDPN) functions in association with the extracellular matrix from the cell surface side. The authors aimed to elucidate the molecular role of PDPN in terms of cell adhesion, proliferation, and migration in oral squamous cell carcinoma cells. [Lab Invest] Abstract Molecular Characterization of the Multiple Interactions of SpsD, a Surface Protein from Staphylococcus pseudintermedius, with Host Extracellular Matrix Proteins The authors describe the cloning and characterization of SpsD, a surface protein of S. pseudintermedius reported as interacting with extracellular matrix proteins and corneocytes. A ligand screen and Western immunoblotting revealed that the N-terminal A domain of SpsD bound fibrinogen, fibronectin, elastin and cytokeratin 10. [PLoS One] Full Article The Inhibitory Effect of Proanthocyanidin on Soluble and Collagen-Bound Proteases Scientists evaluated the inhibitory effect of proanthocyanidin, a natural collagen cross-linker, on soluble and matrix-bound proteases, which are responsible for progressive degradation of exposed collagen fibrils within the hybrid layer and resin-dentine bond failure over time. [J Dent] Abstract Carbodiimide Crosslinked Collagen from Porcine Dermal Matrix for High-Strength Tissue Engineering Scaffold Researchers used carbodiimide to chemically modify collagen derived from porcine acellular dermal matrix (PADM). The results show that the strength and resistance of PADM to enzymatic digestion can be adjusted by the reconnection of free amino and carboxyl groups of the collagen fibers. [Int J Biol Macromol] Abstract Involvement of SDF-1 and Monocyte Chemoattractant Protein-1 in Hydrogen Peroxide-Induced Extracellular Matrix Degradation in Human Dental Pulp Cells Researchers determined whether chemokines such as SDF-1 and monocyte chemoattractant protein-1 are responsible for hydrogen peroxide-induced extracellular matrix degradation and aimed to identify the underlying mechanism in human dental pulp cells. [Int Endod J] Abstract |