M2-Like Macrophages Are Responsible for Collagen Degradation through a Mannose Receptor-Mediated Pathway Tissue remodeling processes critically depend on the timely removal and remodeling of preexisting collagen scaffolds. Nevertheless, many aspects related to the turnover of this abundant extracellular matrix component in vivo are still incompletely understood. Researchers took advantage of recent advances in optical imaging to develop an assay to visualize collagen turnover in situ and identify cell types and molecules involved in this process. [J Cell Biol] Abstract Capillary Morphogenesis in PEG-Collagen Hydrogels Scientists developed a biosynthetic hydrogel consisting of polyethylene glycol diacrylamide conjugated to macromolecular type-I collagen. Through their acrylamide-based crosslinks, these materials allow for independent control of physical properties and bulk ligand concentration. [Biomaterials] Abstract Functional Ultrasound Imaging for Assessment of Extracellular Matrix Scaffolds Used for Liver Organoid Formation A method of 3D functional ultrasound imaging has been developed to enable non-destructive assessment of extracellular matrix scaffolds that have been prepared by decellularization protocols and are intended for recellularization to create organoids. [Biomaterials] Abstract Interdigital Cell Death in the Embryonic Limb Is Associated with Depletion of Reelin in the Extracellular Matrix Scientists showed that Reelin is present in the extracellular matrix of the interdigital mesoderm of chick and mouse embryos during the developmental stages of digit formation. They showed that reelin expression becomes intensely downregulated in both the chick and mouse interdigits preceding the establishment of the areas of interdigital cell death. [Cell Death Dis] Abstract Escherichia coli Biofilms Have an Organized and Complex Extracellular Matrix Structure Investigators showed that uropathogenic Escherichia coli (UPEC) strains produce a biofilm with a highly ordered and complex extracellular matrix. They used electron microscopy techniques to image floating biofilms formed by UPEC. [MBio] Abstract Specific Extracellular Matrix Remodeling Signature of Colon Hepatic Metastases To identify genes implicated in metastatic colonization of the liver in colorectal cancer, the authors collected pairs of primary tumors and hepatic metastases before chemotherapy in 13 patients. Gene Ontology term analysis showed that 50% of the genes are implicated in extracellular matrix remodeling, and more precisely in cell adhesion, extracellular matrix organization and angiogenesis. [PLoS One] Full Article Pivotal Role of Matrix Metalloproteinase 13 in Extracellular Matrix Turnover in Idiopathic Pulmonary Fibrosis Researchers investigated the regulation of matrix metalloproteinases (MMPs) and their inhibitors (TIMPs) in lung fibrosis. MMP and TIMP expression, collagenolytic activity and collagen content was assessed in idiopathic pulmonary fibrosis versus donor lung homogenates and accomplished by in-situ-zymography for gelatinolytic and collagenolytic activities, combined with MMP antigen detection. [PLoS One] Full Article Extracellular Matrix-Mediated Differentiation of Human Embryonic Stem Cells: Differentiation to Insulin-Secreting Beta Cells The authors investigated an alternative strategy for directed differentiation of human embryonic stem cells – extracellular-matrix (ECM) mediated differentiation. Decellularized ECM and conditioned media from the appropriate committed cell lines were used to differentiate stem cells to the required phenotype. [Tissue Eng Part A] Abstract |