Distinct Biophysical Mechanisms of Focal Adhesion Kinase Mechanoactivation by Different Extracellular Matrix Proteins The authors showed by using a genetically encoded focal adhesion kinase (FAK) biosensor based on fluorescence resonance energy transfer, that fibronectin (FN)-mediated FAK activation is dependent on the mechanical tension, which may expose its otherwise hidden FN synergy site to integrin α5. [Proc Natl Acad Sci USA] Abstract Lateral Boundary Mechanosensing by Adherent Cells in a Collagen Gel System Scientists conclude that the presence of physical boundaries interrupts the process of cell-mediated collagen compaction and fiber alignment in the collagen matrix and enhances the formation of cell extensions. This new cell culture platform provides a geometry that more closely approximates the native basement membrane and will help to elucidate the roles of cell extensions and lateral mechanosensing on extracellular matrix remodeling by invasion and degradation. [Biomaterials] Abstract Electrospun Gelatin Scaffolds Incorporating Rat Decellularized Brain Extracellular Matrix for Neural Tissue Engineering Scientists first demonstrated the effectiveness of the proposed decellularization protocol and the cytocompatibility of the resulting brain matrix. Then, the in vitro biological assays of the conditioned electrospun scaffolds, using rat allogeneic mesenchymal stromal cells, confirmed their biocompatibility and showed a differentiative potential in presence of just 1% w/w decellularized rat brain extracellular matrix. [Biomaterials] Abstract Hyaluronic Acid-Based Hydrogels Containing Covalently Integrated Drug Depots: Implication for Controlling Inflammation in Mechanically Stressed Tissues Radically cross-linkable block copolymer micelles assembled from an amphiphilic block copolymer consisting of hydrophilic poly(acrylic acid) partially modified with 2-hydroxyethyl acrylate, and hydrophobic poly(n-butyl acryclate) were employed as the drug depots and the microscopic cross-linkers for the preparation of hyaluronic acid-based, hydrogels. [Biomacromolecules] Abstract | Press Release The Effects of Age and the Expression of SPARC on Extracellular Matrix Production by Cardiac Fibroblasts in 3-D Cultures To determine whether fibroblast production, processing, and deposition of collagen differed with age, primary cardiac fibroblasts from neonate, adult, and old mice were isolated and cultured in 3-dimensional fibrin gels. [PLoS One] Full Article Anti-Connective Tissue Growth Factor Antibody Treatment Reduces Extracellular Matrix Production in Trabecular Meshwork and Lamina Cribrosa Cells Researchers found that FG-3019 is effective in blocking extracellular matrix production in trabecular meshwork and lamina cribrosa cells thus supporting a role for the use of anti-connective tissue growth factor immunotherapy in the treatment of glaucoma. [Invest Ophthalmol Vis Sci] Abstract Return of Motor Function after Repair of a 3-cm Gap in a Rabbit Peroneal Nerve: A Comparison of Autograft, Collagen Conduit, and Conduit Filled with Collagen-GAG Matrix Scientists evaluated the motor nerve recovery in a rabbit model after repair of a 3-cm gap in the peroneal nerve with a conduit filled with a collagen-glycosaminoglycan (GAG) matrix and compared the results with those after reconstruction with an autograft or an empty collagen conduit. [J Bone Joint Surg Am] Abstract Millimeter Wave Promotes the Synthesis of Extracellular Matrix and the Proliferation of Chondrocyte by Regulating the Voltage-Gated K+ Channel While it is unclear if millimeter wave enhances extracellular matrix (ECM) synthesis and proliferation of chondrocytes by regulating K+ channel activity, the authors investigated the effects of millimeter waves on ECM synthesis, chondrocyte proliferation and ion channels in the primary chondrocyte culture. [J Bone Miner Metab] Abstract |