Nonautonomous Contact Guidance Signaling during Collective Cell Migration Although the contact guidance response of individual cells to topographic cues has been extensively characterized, little is known about the response of groups of cells to topographic cues, the impact of such cues on cell-cell coordination within groups, and the transmission of nonautonomous contact guidance information between neighboring cells. Researchers explored these phenomena by quantifying the migratory response of confluent monolayers of epithelial and fibroblast cells to contact guidance cues provided by grooved topography. [Proc Natl Acad Sci USA] Abstract Global Analysis Reveals the Complexity of the Human Glomerular Extracellular Matrix Investigators used mass spectrometry-based proteomics of enriched extracellular matrix (ECM) extracts for a global analysis of human glomerular ECM in vivo and identified a tissue-specific proteome of 144 structural and regulatory ECM proteins. [J Am Soc Nephrol] Full Article Glomerular Cell Cross-Talk Influences Composition and Assembly of Extracellular Matrix To characterize specific cellular contributions to the glomerular basement membrane, scientists used mass spectrometry-based proteomics to analyze extracellular matrix isolated from podocytes and glomerular endothelial cells in vitro. [J Am Soc Nephrol] Full Article The Role of SDF-1α-ECM Crosstalk in Determining Neural Stem Cell Fate The authors investigated the effect of stromal cell-derived factor 1α (SDF-1α) and extracellular matrix (ECM) substrates (Matrigel, laminin, and vitronectin) on the migration, differentiation, and proliferation of neural progenitor/stem cells in vitro using standard assays. [Biomaterials] Abstract Extracellular Matrix Density Regulates the Rate of Neovessel Growth and Branching in Sprouting Angiogenesis Scientists characterized the relationship between matrix density and microvascular topology in an in vitro 3D organ culture model of sprouting angiogenesis. They used these results to design and calibrate a computational growth model to demonstrate how changes in individual neovessel behavior produce the changes in vascular topology that were observed experimentally. [PLoS One] Full Article | Press Release Selective Laser Sintered Poly-ε-Caprolactone Scaffold Hybridized with Collagen Hydrogel for Cartilage Tissue Engineering An in-house selective laser sintering machine was built and 3D poly-ε-caprolactone scaffolds were manufactured using a layer-by-layer design of scaffold struts with varying orientations, producing scaffolds with pores of different shapes and distribution. To better enhance the scaffold properties, chondrocytes were seeded in collagen gel and loaded in scaffolds for cartilage tissue engineering. [Biofabrication] Abstract Extracellular Matrix Density Promotes Epithelial to Mesenchymal Transition by Weakening Cell-Cell Adhesions Researchers developed a computational framework for studying modulation of cell-cell adhesion by extracellular matrix (ECM) density, integrating findings from multiple studies that connect ECM-mediated adhesion signaling and growth factor signaling with cell-cell adhesion. [Mol Biosyst] Abstract A Decorin-Deficient Matrix Affects Skin Chondroitin/Dermatan Sulfate Levels and Keratinocyte Function To determine the impact of the loss of dermatan sulfate-decorin in the dermis, the authors analyzed the glycosaminoglycan content of the decorin gene (Dcn−/−) and wild-type mouse skin. The total amount of chondroitin/dermatan sulfate was increased in the Dcn−/− skin, but was overall less sulfated with a significant reduction in bisulfated ΔDiS2,X (X = 4 or 6) disaccharide units, due to the reduced expression of uronyl 2-O sulfotransferase. [Matrix Biol] Full Article |