Enhanced Ex Vivo Expansion of Adult Mesenchymal Stem Cells by Fetal Mesenchymal Stem Cell ECM Since previous studies have demonstrated superior proliferation and osteogenic potential of human fetal mesenchymal stem cells (fMSCs), researchers hypothesize that their extracellular matrix (ECM) may promote expansion of clinically relevant aMSCs. They showed that adult human MSCs (aMSCs) were more proliferative on fMSC-derived ECM than aMSC-derived ECMs and traditional tissue culture wares. [Biomaterials] Full Article Interaction of the Vinculin Proline-Rich Linker Region with Vinexin α in Sensing Extracellular Matrix Stiffness Scientists showed that the proline-rich linker (PRL) region of vinculin and the PRL region-binding protein vinexin are involved in sensing stiffness of extracellular matrix (ECM) substrates. A rigid substrate increases cytoskeleton-associated vinculin, and the fraction of vinculin stably localizing at focal adhesions is larger on rigid ECM than on soft ECM. [J Cell Sci] Abstract A Functional Polyester Carrying Free Hydroxyl Groups Promotes the Mineralization of Osteoblast and Human Mesenchymal Stem Cell Extracellular Matrix Investigators examined the use of poly(sebacoyl diglyceride) (PSeD) to promote biomineralization for application in bone tissue engineering. PSeD promoted more mineralization of extracellular matrix secreted by human mesenchymal stem cells and rat osteoblasts than poly(lactic-co-glycolic acid), which is currently widely used in bone tissue engineering. [Acta Biomater] Abstract Collagen-GAG Scaffold Biophysical Properties Bias MSC Lineage Choice in the Presence of Mixed Soluble Signals Scientists examined the role of biomaterial physical properties (relative density, mineral content) on human mesenchymal stem cell (MSC) phenotype in the presence of mixed soluble signals to drive osteogenesis or chondrogenesis. They tested a series of 3D collagen-glycosaminoglycan scaffolds with properties inspired by extracellular matrix characteristics across the osteotendinous interface (tendon, cartilage, and bone). [Tissue Eng Part A] Abstract Microporous Dermal-Like Electrospun Scaffolds Promote Accelerated Skin Regeneration The authors aimed to synthesize skin substitutes that blend native extracellular matrix molecules (ECM) with synthetic polymers that have favorable mechanical properties. To this end, scaffolds were electrospun from collagen I and polycaprolactone, and then pores were introduced mechanically to promote fibroblast infiltration, and subsequent filling of the pores with ECM. [Tissue Eng Part A] Abstract Dendritic Cells: In Vitro Culture in Two- and Three-Dimensional Collagen Systems and Expression of Collagen Receptors in Tumors and Atherosclerotic Microenvironments The authors have previously shown that the interaction of dendritic cells (DCs) with various extracellular matrix components modifies the immune properties and angiogenic potential of these cells. Building on those studies, they analyzed the angiogenic profile of murine myeloid DCs upon interaction with 2D and 3D type-I collagen environments. [Exp Cell Res] Abstract The Predicted Secretomes of Monosiga brevicollis and Capsaspora owczarzaki, Close Unicellular Relatives of Metazoans, Reveal New Insights into the Evolution of the Metazoan Extracellular Matrix Researchers analyzed all predicted secretory signal-peptide-containing proteins of M. brevicollis for extracellular matrix (ECM) domains. Nine domains that are widespread in metazoan ECM proteins are represented, with EGF, fibronectin III, laminin G, and von Willebrand Factor_A domains being the most numerous. [Matrix Biol] Abstract Redirection of Neurite Outgrowth by Coupling Chondroitin Sulfate Proteoglycans to Polymer Membranes Researchers examined the potential of chondroitin sulfate proteoglycans (CSPGs) to guide dorsal root ganglia (DRG) neurite outgrowth when freely available in the media or presented from a polymeric membrane. Soluble CSPGs added to the media of DRG explant cultures inhibited neurite outgrowth without spatial bias, caused retraction of axons, and decreased neurite extension in a dose-dependent manner. [Ann Biomed Eng] Abstract Extracellular Matrix Components Mark the Territories of Circumventricular Organs Scientists investigate tenascin-R and the lecticans: aggrecan, brevican, neurocan, and versican in the median eminence, the area postrema, the vascular organ of the lamina terminalis, the subfornical organ, the pineal body and the subcommissural organ of the rat applying immunohistochemical methods, and lectin histochemistry, using Wisteria floribunda agglutinin. [Neurosci Lett] Abstract |