Micropattern Width Dependent Sarcomere Development in Human Embryonic Stem Cell-Derived Cardiomyocytes High-resolution photolithography techniques and microcontact printing were utilized to produce features of various rectangular geometries, with areas ranging from 2500 μm2 to 160,000 μm2. The microcontact printing method was used to pattern non-adherent poly(ethylene glycol) regions on gold coated glass slides. Matrigel and fibronectin extracellular matrix proteins were layered onto the gold-coated glass slides, providing a controlled geometry for cell adhesion. [Biomaterials] Abstract Topologically Defined Composites of Collagen Type I and V as In Vitro Cell Culture Scaffolds Scientists aimed to provide an easy-accessible biomimetic in vitro platform of topologically defined collagen I matrices to dissect cell behavior under various conditions in vitro. The authors reconstituted covalently bound layers of three-dimensional networks of collagen type I and collagen type V with a defined network topology. [Acta Biomater] Abstract Sulfatide-Mediated Control of Extracellular Matrix-Dependent Oligodendrocyte Maturation Scientists investigated whether sulfatide interferes with extracellular matrix-integrin interactions and, in this manner, modulates oligodendrocyte (OLG) maturation. Their data reveal that disruption of laminin-2-sulfatide interactions impeded OLG differentiation and myelin-like membrane formation. [Glia] Abstract Staurosporine and Extracellular Matrix Proteins Mediate the Conversion of Small Cell Lung Carcinoma Cells into a Neuron-Like Phenotype Researchers showed that four out of five small cell lung carcinoma cell lines reversibly develop a neuron-like phenotype on extracellular matrix constituents such as fibronectin, laminin or thrombospondin upon staurosporine treatment in an RGD/integrin-mediated manner. [PLoS One] Full Article Monitoring Fibrous Scaffold Guidance of Three-Dimensional Collagen Organization Using Minimally-Invasive Second Harmonic Generation Electrospun fibers with a random or high degree of orientation, mimicking two types of tissue architecture found in the body, were used to culture human fibroblasts for controlling cell alignment. The minimally-invasive technique of second harmonic generation was used with the aim of monitoring and profiling the deposition and organization of collagen at different construct depths over time while construct mechanical properties were also determined over the culture period. [PLoS One] Full Article Dentin Matrix Proteins (DMPs) Enhance Differentiation of BMMSCs via ERK and P38 MAPK Pathways Non-collagenous DMPs were isolated from healthy human teeth and their effects on bone marrow stromal stem cells (BMMSCs) behavior were examined during in vitro culture. [Cell Tissue Res] Abstract Repair of an Unusual Aortic Coarctation Using an Extracellular Matrix Patch Investigators describe an aortic coarctation with an uncommon anatomic aspect treated with a CorMatrix (CorMatrix, Alpharetta, GA) extracellular matrix patch. [Ann Thorac Surg] Abstract Galectin-1-Mediated Cell Adhesion, Invasion and Cell Death in Human Anaplastic Large Cell Lymphoma: Regulatory Roles of Cell Surface Glycans To elucidate the biological roles of galectin-1 in cell adhesion and invasion of human anaplastic large cell lymphoma, the authors performed cell adhesion and invasion assays using the anaplastic large cell lymphoma cell line H-ALCL, which was previously established in their laboratory. [Int J Oncol] Abstract | Download Full Article |