Epidermal Cells Help Coordinate Leukocyte Migration during Inflammation through Fatty Acid-Fuelled Matrix Metalloproteinase Production By live imaging metabolic and immunological processes within intact zebrafish embryos during cutaneous inflammation, investigators uncovered a mechanism where elevated β-oxidation-fuelled mitochondria-derived reactive oxygen species within epidermal cells helps guide matrix metalloproteinase-driven leukocyte recruitment. [Nat Commun] Abstract Capillary-Wall Collagen as a Biophysical Marker of Nanotherapeutic Permeability into the Tumor Microenvironment Using a computational model along with in vivo systems, researchers compared how collagen structure affects the diffusion flux of a 1 nm chemotherapeutic molecule and an 80 nm chemotherapy-loaded pegylated liposome in tumor vasculature. [Cancer Res] Abstract Macrophage Polarization in Response to ECM Coated Polypropylene Mesh Spatiotemporal analysis of macrophage polarization was conducted in response to uncoated polypropylene mesh and mesh coated with hydrated and dry forms of extracellular matrix (ECM) hydrogels derived from either dermis or urinary bladder. [Biomaterials] Abstract Snail1 Induced in Breast Cancer Cells in 3D Collagen I Gel Environment Suppresses Cortactin and Impairs Effective Invadopodia Formation Investigators explored the behaviors of MDA-MB-231 breast cancer cells embedded in 3D collagen I. Diverse tumor environmental conditions (including cell density, extracellular acidity, or hypoxia as mimics for a continuous tumor growth) reduced JNKs, enhanced TGFβ1/Smad signaling activity, induced Snail1, and reduced cortactin expression. [Biochim Biophys Acta] Abstract Banded Structures in Collagen Vitrified Gels for Corneal Injury Repair There is a growing interest in using collagen vitrified gels for corneal injury repair. The authors recently reported the synthesis and thermal denaturation behavior of these collagen vitrified gels. In this paper, the banded structure in these vitrified gels was studied by small-angle x-ray scattering one dimensional correlation function analysis and transmission electron microscopy. [Acta Biomater] Abstract Proteomics of Fuchs’ Endothelial Corneal Dystrophy Support that the Extracellular Matrix of Descemet’s Membrane Is Disordered As the morphological changes in Fuchs’ endothelial corneal dystrophy are mainly observed in the extracellular matrix of the Descemet’s membrane/endothelial layer, scientists determined the protein profiles of diseased and control tissues using two relative quantitation MS methods. [J Proteome Res] Abstract The Metastatic Potential of Triple-Negative Breast Cancer Is Decreased via Caloric Restriction-Mediated Reduction of the miR-17~92 Cluster Scientists sought to determine if the molecular interaction between caloric restriction (CR) and ionizing radiation (IR) could be mediated by microRNA (miR). miR arrays revealed three miRs in the miR-17~92 cluster as most significantly down regulated when CR is combined with IR. To elucidate the mechanism by which this cluster regulates the response to CR, cDNA arrays were performed and the top five statistically significant gene ontology terms with high fold changes were all associated with extracellular matrix and metastases. [Breast Cancer Res Treat] Abstract | Press Release 3D Extracellular Matrix Scaffolds by Microfluidic Fabrication for Long-Term Spontaneously Contracted Cardiomyocytes Culture Primary neonatal mice cardiomyocytes were successfully harvested and cultured in 3D scaffolds made of gelatin and collagen. Gelatin and gelatin-collagen scaffold were produced by the formation of microbubbles through a microfluidic device and the mechanical properties of gelatin scaffold and gelatin-collagen scaffold were measured. [Tissue Eng Part A] Abstract Excreted/Secreted Schistosoma mansoni Venom Allergen-Like 9 (SmVAL9) Modulates Host Extracellular Matrix Remodeling Gene Expression Scientists demonstrate that recombinant SmVAL9 affects the expression of extracellular matrix, remodeling matrix metalloproteinase and tissue inhibitors of metalloproteinase gene products in both Biomphalaria glabrata embryonic cell and Mus musculus bone marrow-derived macrophage in vitro cultures. [Int J Parasitol] Full Article Structural Characterization and Interaction of Periostin and Bone Morphogenetic Protein for Regulation of Collagen Cross-Linking Periostin has the ability to associate with other critical extracellular matrix regulators such as transforming growth factor-β, tenascin, and fibronectin, and is a critical regulator of fibrosis that functions by altering the deposition and attachment of collagen. To investigate the molecular interaction of periostin and bone morphogenetic protein, researchers modeled these three-dimensional structures and their binding sites. [Biochem Biophys Res Commun] Abstract |