DNA-Based Digital Tension Probes Reveal Integrin Forces during Early Cell Adhesion Researchers developed a new class of molecular tension probes that function as a switch to generate a 20- to 30-fold increase in fluorescence upon experiencing a threshold piconewton force. Quantitative imaging revealed that integrin tension is highly dynamic and increases with an increasing integrin density during adhesion formation. [Nat Commun] Abstract | Press Release B-LINK: A Hemicentin, Plakin, and Integrin-Dependent Adhesion System that Links Tissues by Connecting Adjacent Basement Membranes Using live-cell imaging and electron microscopy, researchers identified an adhesion system that connects the uterine and gonadal tissues through their juxtaposed basement membranes at the site of anchor cell invasion in Caenorhabditis elegans. [Dev Cell] Abstract | Graphical Abstract Developing Functional Musculoskeletal Tissues through Hypoxia and Lysyl Oxidase-Induced Collagen Cross-Linking Direct administration of exogenous lysyl oxidase (LOX) was applied in native cartilage and neocartilage generated using a scaffold-free, self-assembling process of primary chondrocytes. Exogenous LOX was found to enhance native tissue tensile properties 1.9-fold. [Proc Natl Acad Sci USA] Abstract LKB1 Loss in Melanoma Disrupts Directional Migration toward Extracellular Matrix Cues Using a microfluidic gradient chamber system and time-lapse microscopy scientists uncover a new function for LKB1 as a directional migration sensor of gradients of extracellular matrix but not soluble growth factor cues. [J Cell Biol] Abstract Tenascin-C Mimetic Peptide Nanofibers Direct Stem Cell Differentiation to Osteogenic Lineage Scientists achieved control of stem cell fate towards osteogenic commitment by using a single extracellular matrix element despite the contradictory effect of mechanical stiffness. For this purpose, they mimicked bone extracellular matrix by incorporating functional sequence of fibronectin type III domain from native tenascin-C on self-assembled peptide nanofibers. [Biomacromolecules] Abstract Influence of the Mechanical Environment on the Engineering of Mineralized Tissues Using Human Dental Pulp Stem Cells and Silk Fibroin Scaffolds Researchers evaluated the mineralization potential of human dental pulp stem cells seeded on porous silk fibroin scaffolds in a mechanically dynamic environment provided by spinner flask bioreactors. [PLoS One] Full Article Multiple Roles of Extracellular Fibroblast Growth Factors in Lung Cancer Cells Scientists aimed to determine whether extracellular fibroblast growth factors (FGFs) can affect the biology of lung cancer cells and to understand how extracellular FGFs affect the biology of lung cancer cells, including non-small cell lung cancer and small cell lung cancer cells. [Int J Oncol] Abstract Smad2 Protects against TGF-β1/Smad3-Mediated Collagen Synthesis in Human Hepatic Stellate Cells during Hepatic Fibrosis Researchers demonstrate that knockdown of Smad2 in human hepatic stellate cells increased expression of collagen I, tissue inhibitor of metalloproteinase-1 whereas decreasing expression of the matrix metalloproteinases-2 in presence of transforming growth factor-β1 (TGF-β1) compared with control group. [Mol Cell Biochem] Abstract Three-Dimensional Constructs Using Hyaluronan Cell Carrier as a Tool for the Study of Cancer Stem Cells U87 astrocytoma cells were grown on polycaprolactone (PCL)-2 dimensional flat substrates and PCL-3 dimensional scaffolds (3D) eventually containing hyaluronic acid (HA). Cell viability, growth patterns, morphology, and cell surface marker expression were studied to assess the effect of 3D culture and presence of HA. [J Biomed Mater Res B Appl Biomater] Abstract Extracellular Matrix-Immobilized Nanotopographical Substrates for Enhanced Myogenic Differentiation Researchers demonstrate extracellular matrix immobilization on polyurethane acrylate substrate fabricated in 24 well-plate platforms to effectively differentiate C2C12. They demonstrate that co-immobilization of fibronectin (FN) and laminin (LMN) was found to enhance myogenic differentiation of C2C12 cells compared to single immobilization of either FN or LMN alone. [J Biomed Mater Res B Appl Biomater] Abstract |