p70 S6-Kinase Mediates the Cooperation between Akt1 and Mek1 Pathways in Fibroblast-Mediated Extracellular Matrix Remodeling Scientists investigated the specific role of Akt1 and Mek1 in extracellular matrix (ECM) synthesis and assembly by NIH 3T3 fibroblasts and how these effects were reconciled to mediate overall ECM remodeling. They identified that cooperation between Akt1 and Mek1 is necessary to mediate ECM synthesis. [Biochim Biophys Acta] Abstract Cardiomyoblast (H9c2) Differentiation on Tunable Extracellular Matrix (ECM) Microenvironment The authors investigated cardiomyogenic potential of fibroblast-derived matrix as a novel ECM platform over gelatin or fibronectin, in generating cardiac cell lineages derived from H9c2 cardiomyoblasts. [Tissue Eng Part A] Abstract Retention of the Stemness of Mouse Adipose Derived Stem Cells by Their Expansion on Human Bone Marrow Stromal Cell Derived Extracellular Matrix Previously, coating with bone marrow mesenchymal stem cells (MSCs) derived extracellular matrix (ECM) was found able to maintain the differentiation potential of in vitro cultured MSCs. Researchers determined if this ECM coating could also maintain the stemness of cultured murine adipose derived stem cells using regular culture flask as a control. [Tissue Eng Part A] Abstract The Effect of Substrate Stiffness, Thickness, and Cross-Linking Density on Osteogenic Cell Behavior Scientists characterized the local stiffness at the cellular level for MC3T3-E1 cells plated on biomaterial substrates of varying modulus, thickness, and cross-linking concentration. MC3T3 cells cultured on a soft fibrous substrate attained the same spread cell area as those cultured on a much higher modulus, but nonfibrous substrate. [Biophys J] Abstract Matrix Metalloproteinase-9 Expression Is Enhanced in Renal Parietal Epithelial Cells of Zucker Diabetic Fatty Rats and Is Induced by Albumin in In Vitro Primary Parietal Cell Culture Researchers investigated whether and how matrix metalloproteinase-9 expression and activity were changed in glomerular epithelial cells upon albumin overload. [PLoS One] Full Article Interaction between Fibronectin and β1 Integrin Is Essential for Tooth Development To determine the role of fibronectin in differentiation of the dental epithelium and tooth formation, scientists analyzed its expression in developing incisors. [PLoS One] Full Article Anisotropic Silk Biomaterials Containing Cardiac Extracellular Matrix for Cardiac Tissue Engineering Investigators developed a silk-based scaffold platform containing cardiac tissue-derived extracellular matrix (cECM). These silk-cECM composite scaffolds had tunable architectures, degradation rates, and mechanical properties. [Biomed Mater] Abstract Self-Synthesized Extracellular Matrix Contributes to Mature Adipose Tissue Regeneration in a Tissue Engineering Chamber The authors describe a chamber-based system for improving the yield of mature adipose tissue and discuss the potential mechanism of adipogenesis in tissue-chamber models. [Wound Repair Regen] Abstract Design of an Osteoinductive Extracellular Fibronectin Matrix Protein for Bone Tissue Engineering Investigators constructed a novel osteoinductive fibronectin matrix protein for bone tissue engineering, designed to combine the integrin-binding modules from fibronectin and a strong osteoinductive growth factor, bone morphogenetic protein-2. [Int J Mol Sci] Abstract | Download Full Article |