Adaptive Rheology and Ordering of Cell Cytoskeleton Govern Matrix Rigidity Sensing Researchers showed that rigidity sensing and adaptation emerge naturally from actin cytoskeleton remodeling. Their in vitro experiments and theoretical modelling demonstrated a biphasic rheology of the actin cytoskeleton, which transitions from fluid on soft substrates to solid on stiffer ones. [Nat Commun] Abstract Filamin A Mediates Wound Closure by Promoting Elastic Deformation and Maintenance of Tension in the Collagen Matrix Investigators examined the role of filamin A (FLNa), an actin filament cross-linking protein, in wound contraction and maintenance of matrix tension. Conditional deletion of FLNa in fibroblasts in mice was associated with approximately four days delay of full-thickness skin wound contraction compared with wild-type mice. [J Invest Dermatol] Abstract Elevated Expression of Periostin in Human Osteoarthritic Cartilage and Its Potential Role in Matrix Degradation via Matrix Metalloproteinase-13 Scientists investigated the role of periostin, an extracellular matrix protein, in the pathophysiology of osteoarthritis (OA). They examined periostin expression by immunohistochemical analysis of lesional and nonlesional cartilage from human and rodent OA knee cartilage. [FASEB J] Abstract Pathophysiological Role of MicroRNA-29 in Pancreatic Cancer Stroma In an effort to understand the molecular mechanisms associated with tumor-stromal interactions, using in vitro and in vivo models and pancreatic ductal adenocarcinoma patient biopsies, scientists showed that the loss of miR-29 is a common phenomenon of activated pancreatic stellate cells/fibroblasts, the major stromal cells responsible for fibrotic stromal reaction. [Sci Rep] Full Article | Press Release Cantharidin Represses Invasion of Pancreatic Cancer Cells through Accelerated Degradation of MMP2 mRNA Investigators found that cantharidin repressed the invasive ability of pancreatic cancer cells and downregulated matrix metalloproteinase 2 (MMP2) expression through multiple pathways, including ERK, JNK, PKC, NF-κB, and β-catenin. [Sci Rep] Full Article Controlled Growth and the Maintenance of Human Pluripotent Stem Cells by Cultivation with Defined Medium on Extracellular Matrix-Coated Micropatterned Dishes Researchers introduced a micropatterned dish that was coated with extracellular matrix by photolithographic technology. This procedure allowed the cultivation of human pluripotent stem cells on 199 individual recombinant human vitronectin-N-coated small round spots on each 35-mm polystyrene dish, permitting the simultaneous formation of 199 uniform high-density small-sized colonies. [PLoS One] Full Article Alcohol Differentially Alters Extracellular Matrix and Adhesion Molecule Expression in Skeletal Muscle and Heart Scientists used an unbiased discovery-based approach to determine the effect of chronic alcohol consumption on the expression profile of genes important for cell–cell and cell–extracellular matrix interactions in both skeletal and cardiac muscle. [Alcohol Clin Exp Res] Abstract Extracellular Matrix-Based Intracortical Microelectrodes: Toward a Microfabricated Neural Interface Based on Natural Materials Extracellular matrix (ECM)-based implantable neural electrodes were achieved using a microfabrication strategy on natural-substrate-based organic materials. The ECM-based design minimized the introduction of non-natural products into the brain. [Microsyst Nanoeng] Full Article |