Biodegradable Interstitial Release Polymer Loading a Novel Small Molecule Targeting Axl Receptor Tyrosine Kinase and Reducing Brain Tumor Migration and Invasion Scientists developed a controlled-release polyanhydride polymer loading a novel small molecule, n‑butylidenephthalide (BP), which is not only increasing local drug concentration and extending its diffusion distance but also reducing tumor invasion, mediated by reducing Axl expression. BP downregulated matrix metalloproteinase activity, which is involved in cancer cell invasion. [Oncogene] Full Article E-Cadherin-Defective Gastric Cancer Cells Depend on Laminin to Survive and Invade Investigators hypothesized that deregulation of cell-matrix interactions could play an important role during the invasive process associated to epithelial-cadherin (Ecad) dysfunction. Thus, they focused on LM-332, which is a major matrix component, and on Ecad/LM-332 crosstalk in the process of Ecad-dependent invasion. [Hum Mol Genet] Abstract Semaphorin 3D Autocrine Signaling Mediates the Metastatic Role of Annexin A2 in Pancreatic Cancer Scientists found that annexin A2 increased the release of semaphorin 3D from pancreatic ductal adenocarcinoma (PDA) cells and promoted migratory behavior in cultured PDA cells. [Sci Signal] Abstract | Press Release Decellularized Human Liver as a Natural 3D-Scaffold for Liver Bioengineering and Transplantation Investigators demonstrated complete decellularization of whole human liver and lobes to form an extracellular matrix scaffold with a preserved architecture. Decellularized human liver cubic scaffolds were repopulated for up to 21 days using human cell lines hepatic stellate cells, hepatocellular carcinoma and hepatoblastoma, with excellent viability, motility and proliferation and remodeling of the extracellular matrix. [Sci Rep] Full Article Validated Prediction of Pro-Invasive Growth Factors Using a Transcriptome-Wide Invasion Signature Derived from a Complex 3D Invasion Assay Invading fibroblasts contribute to fibrotic extracellular matrix formation and the initiation, progression, or resistance of cancer. To construct transcriptome-wide signatures of fibroblast invasion, researchers used a multiplex phenotypic 3D invasion assay using lung fibroblasts. [Sci Rep] Full Article CD44 Influences Fibroblast Behaviors via Modulation of Cell-Cell and Cell-Matrix Interactions, Affecting Survivin and Hippo Pathways The authors report findings on the roles of CD44 in modulating proliferation, apoptosis, migration and invasion of murine wild-type and CD44 knockout dermal fibroblasts (CD44KO-FB). As documented in microvascular endothelial cells lacking CD44, they found persistent increased proliferation, reduced activation of cleaved caspase 3, increased initial attachment, but decreased strength of cell attachment in high cell density, post confluent CD44KO-FB cultures. [J Cell Physiol] Abstract Combination of Agrin and Laminin Increase Acetylcholine Receptor Clustering and Enhance Functional Neuromuscular Junction Formation In Vitro Researchers investigated the effect of agrin and laminin-1, agents with known acetylcholine receptors clustering activity, on neuromuscular junctions formation and muscle maturation. Primary myoblasts were differentiated in vitro on collagen, laminin or collagen and laminin-coated surfaces in the presence or absence of agrin and laminin. [Dev Neurobiol] Abstract Optimizing Contraction and Alignment of Cellular Collagen Hydrogels to Achieve Reliable and Consistent Engineered Anisotropic Tissue Scientists developed an assay system based on contraction of free-floating cellular gels in 96-well plates that could be used to investigate cell–matrix interactions and established optimal parameters for subsequent self-alignment of cells in tethered gels. [J Biomater Appl] Abstract Activin-A Signaling Promotes Epithelial–Mesenchymal Transition, Invasion, and Metastatic Growth of Breast Cancer Scientists report that activin-A signaling is hyperactivated in breast cancers as indicated by higher activin-A, phosphoSMAD2, and phosphoSMAD3 levels in advanced breast cancers. Activin-A promotes anchorage-independent growth, epithelial–mesenchymal transition, invasion, angiogenesis, and stemness of breast cancer cells. [npj Breast Cancer] Full Article |